PMID- 30689826
OWN - NLM
STAT- MEDLINE
DCOM- 20191022
LR  - 20191022
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 366
IP  - 4
DP  - 2019 Feb 1
TI  - High frequency of coagulase-positive staphylococci carriage in healthy wild boar 
      with detection of MRSA of lineage ST398-t011.
LID - fny292 [pii]
LID - 10.1093/femsle/fny292 [doi]
AB  - The objective of this study was to determine the frequency and diversity of 
      coagulase-positive staphylococci (CoPS) in nasal samples of healthy wild boar, to 
      study their resistance phenotypes/genotypes and to check the occurrence of the 
      MRSA-ST398. Nasal samples of 371 wild boars were collected in Spain for 
      staphylococci and MRSA recovery. Staphylococci identification was performed by 
      matrix-assisted laser desorption/ionization time of flight mass spectrometry 
      (MALDI-TOF). The susceptibility to 11 antimicrobials was tested by disc-diffusion 
      and the presence of resistance genes by PCR. Molecular typing and virulence 
      factors determination were carried out by PCR and sequencing. The rate of CoPS 
      carriage (Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus 
      pseudintermedius) in wild boar was of 17.8% (13.7%, 2.7% and 1.6%, respectively). 
      Susceptibility to all tested antimicrobials was shown in 74.5% of S. aureus and 
      one strain was MRSA [lineage ST398-t011-agrI, carrying blaZ, mecA, tet(M) and 
      tet(K) genes]. A total of 22 spa-types and 17 STs were detected among S. aureus, 
      including: ST398/CC398 (n = 1), ST2328-ST133/CC133 (n = 20), ST425/CC425 (n = 7), 
      ST5/CC5 (n = 5), ST1/CC1 (n = 3), ST130/CC130 (n = 2) and ST88/CC88 (n = 1). Two 
      spa-types (t02, t15) and four STs (ST455, ST796, ST797, ST798) were detected 
      among the six S. pseudintermedius isolates recovered, and all of them carried the 
      lukF/S-I and siet virulence genes. All S. hyicus isolates were susceptible to 
      antimicrobials tested.
CI  - (c) FEMS 2019.
FAU - Mama, Olouwafemi Mistourath
AU  - Mama OM
AD  - Area de Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, Madre 
      de Dios 51, 26006 Logrono, Spain.
FAU - Ruiz-Ripa, Laura
AU  - Ruiz-Ripa L
AD  - Area de Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, Madre 
      de Dios 51, 26006 Logrono, Spain.
FAU - Fernandez-Fernandez, Rosa
AU  - Fernandez-Fernandez R
AD  - Area de Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, Madre 
      de Dios 51, 26006 Logrono, Spain.
FAU - Gonzalez-Barrio, David
AU  - Gonzalez-Barrio D
AD  - Grupo SaBio, Instituto de Investigacion en Recursos Cinegeticos IREC 
      (CSIC-UCLM-JCCM), Ronda de Toledo, 12 13071 Ciudad Real Espana, Spain.
FAU - Ruiz-Fons, Jose Francisco
AU  - Ruiz-Fons JF
AD  - Grupo SaBio, Instituto de Investigacion en Recursos Cinegeticos IREC 
      (CSIC-UCLM-JCCM), Ronda de Toledo, 12 13071 Ciudad Real Espana, Spain.
FAU - Torres, Carmen
AU  - Torres C
AD  - Area de Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, Madre 
      de Dios 51, 26006 Logrono, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coagulase)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Typing Techniques
MH  - Coagulase/metabolism
MH  - Drug Resistance, Multiple, Bacterial/genetics
MH  - Methicillin-Resistant Staphylococcus aureus/classification/*physiology
MH  - Prevalence
MH  - Spain/epidemiology
MH  - Staphylococcal Infections/*epidemiology/*microbiology
MH  - Staphylococcus/drug effects/enzymology/*physiology
MH  - Sus scrofa/*microbiology
MH  - Swine
MH  - Swine Diseases/*epidemiology/*microbiology
MH  - Virulence Factors/genetics
OTO - NOTNLM
OT  - S. aureus
OT  - S. hyicus
OT  - S. pseudintermedius
OT  - CC398
OT  - MRSA
OT  - wild boar
EDAT- 2019/01/29 06:00
MHDA- 2019/10/23 06:00
CRDT- 2019/01/29 06:00
PHST- 2018/09/12 00:00 [received]
PHST- 2018/12/20 00:00 [accepted]
PHST- 2019/01/29 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2019/01/29 06:00 [entrez]
AID - 5301339 [pii]
AID - 10.1093/femsle/fny292 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2019 Feb 1;366(4):fny292. doi: 10.1093/femsle/fny292.