PMID- 30691219
OWN - NLM
STAT- MEDLINE
DCOM- 20190404
LR  - 20211204
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 11
IP  - 2
DP  - 2019 Jan 27
TI  - Anti-Inflammatory Effect of Chloroform Fraction of Pyrus Ussuriensis Maxim. Leaf 
      Extract on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis in nc/nga Mice.
LID - 10.3390/nu11020276 [doi]
LID - 276
AB  - Pyrus ussuriensis Maxim, a pear commonly known as "Sandolbae" in Korea, is used 
      as a traditional herbal medicine for asthma, cough, and fever in Korea, China, 
      and Japan. P. ussuriensis Maxim leaves (PUL) have therapeutic effects on atopic 
      dermatitis (AD). However, there are no reports on the efficacy of specific 
      components of PUL. In the present study, activity-guided isolation of PUL was 
      used to determine the compounds with potent activity. Astragalin was identified 
      as the major component of the chloroform-soluble fraction of PUL (PULC) using 
      High-performance liquid chromatography (HPLC) analysis. Astragalin and PULC were 
      tested in vitro and in vivo for their effects against AD. PULC and astragalin 
      dose-dependently inhibited the production of nitric oxide (NO) in mouse 
      macrophage (RAW 264.7) cells, and interleukin (IL)-6 and IL-1beta in tumor necrosis 
      factor (TNF-alpha)/interferon gamma (IFNgamma) induced HaCaT cells. In the AD mice model, 
      PULC and astragalin application significantly reduced dermatitis severity, 
      scratching behavior, and trans-epidermal water loss (TEWL) when compared to that 
      of 2, 4-dinitrochlorobenzene-treated NC/Nga mice. Additionally, they normalized 
      skin barrier function by decreasing immunoglobulin E (IgE) levels in the serum. 
      Filaggrin and involucrin protein levels were normalized by PULC treatment in 
      HaCaT cells and skin lesions. These results indicate that PULC and astragalin 
      ameliorate AD-like symptoms by alleviating both pro-inflammatory cytokines and 
      immune stimuli in vitro and in vivo in animal models. Therefore, PULC and 
      astragalin might be effective therapeutic agents for the treatment of AD.
FAU - Cho, KyoHee
AU  - Cho K
AD  - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 
      21936, Korea. kcho3138@gmail.com.
AD  - Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, 
      Yeonsu-gu, Incheon 21936, Korea. kcho3138@gmail.com.
FAU - Kang, Min Cheol
AU  - Kang MC
AD  - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 
      21936, Korea. mincjf07@gmail.com.
AD  - Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, 
      Yeonsu-gu, Incheon 21936, Korea. mincjf07@gmail.com.
FAU - Parveen, Amna
AU  - Parveen A
AD  - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 
      21936, Korea. amnaparvin@gmail.com.
AD  - Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, 
      Yeonsu-gu, Incheon 21936, Korea. amnaparvin@gmail.com.
AD  - Department of Pharmacognosy, Faculty of Pharmaceutical Science, Government 
      College University, Faisalabad 38000, Pakistan. amnaparvin@gmail.com.
FAU - Yumnam, Silvia
AU  - Yumnam S
AD  - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 
      21936, Korea. silviayumnam@gmail.com.
AD  - Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, 
      Yeonsu-gu, Incheon 21936, Korea. silviayumnam@gmail.com.
FAU - Kim, Sun Yeou
AU  - Kim SY
AUID- ORCID: 0000-0001-8044-5613
AD  - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 
      21936, Korea. sunnykim@gachon.ac.kr.
AD  - Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, 
      Yeonsu-gu, Incheon 21936, Korea. sunnykim@gachon.ac.kr.
AD  - Gachon Medical Research Institute, Gil Medical Center, Incheon 21565, Korea. 
      sunnykim@gachon.ac.kr.
LA  - eng
GR  - NIBR201828201/National Institute of Biological Resources/
PT  - Journal Article
DEP - 20190127
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Kaempferols)
RN  - 0 (Plant Extracts)
RN  - 7V31YC746X (Chloroform)
RN  - 82115-62-6 (Interferon-gamma)
RN  - APM8UQ3Z9O (astragalin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Behavior, Animal/drug effects
MH  - Cell Line
MH  - Chloroform/chemistry
MH  - Dermatitis, Atopic/chemically induced/*metabolism/pathology
MH  - Dinitrochlorobenzene/adverse effects
MH  - Filaggrin Proteins
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-6/metabolism
MH  - Kaempferols/analysis
MH  - Mice
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Pyrus/*chemistry
MH  - RAW 264.7 Cells
MH  - Skin/drug effects/metabolism/pathology
PMC - PMC6412787
OTO - NOTNLM
OT  - DNCB-induced-NC/Nga mice
OT  - IL-6
OT  - PULC
OT  - TNF-alpha/IFNgamma
OT  - astragalin
OT  - atopic dermatitis
COIS- The authors declare no conflict of interest.
EDAT- 2019/01/30 06:00
MHDA- 2019/04/05 06:00
PMCR- 2019/02/01
CRDT- 2019/01/30 06:00
PHST- 2019/01/02 00:00 [received]
PHST- 2019/01/15 00:00 [revised]
PHST- 2019/01/23 00:00 [accepted]
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/04/05 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - nu11020276 [pii]
AID - nutrients-11-00276 [pii]
AID - 10.3390/nu11020276 [doi]
PST - epublish
SO  - Nutrients. 2019 Jan 27;11(2):276. doi: 10.3390/nu11020276.