PMID- 30691253
OWN - NLM
STAT- MEDLINE
DCOM- 20190426
LR  - 20190426
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 29
IP  - 3
DP  - 2019 Mar 28
TI  - Oxidized Carbon Nanosphere-Based Subunit Vaccine Delivery System Elicited Robust 
      Th1 and Cytotoxic T Cell Responses.
PG  - 489-499
LID - 10.4014/jmb.1809.09049 [doi]
AB  - Subunit vaccines are safer and more stable than live vaccines although they have 
      the disadvantage of eliciting poor immune response. To develop a subunit vaccine, 
      an effective delivery system targeting the key elements of the protective immune 
      response is a prerequisite. In this study, oxidized carbon nanospheres (OCNs) 
      were used as a subunit vaccine delivery system and tuberculosis (TB) was chosen 
      as a model disease. TB is among the deadliest infectious diseases worldwide and 
      an effective vaccine is urgently needed. The ability of OCNs to deliver 
      recombinant Mycobacterium tuberculosis (Mtb) proteins, Ag85B and HspX, into bone 
      marrow derived macrophages (BMDMs) and dendritic cells (BMDCs) was investigated. 
      For immunization, OCNs were mixed with the two TB antigens as well as the 
      adjuvant monophosphoryl lipid A (MPL). The protective efficacy was analyzed in 
      vaccinated mice by aerosol Mtb challenge with a virulent strain of Mtb and the 
      bacterial burdens were measured. The results showed that OCNs are highly 
      effective in delivering Mtb proteins into the cytosol of BMDMs and BMDCs. Upon 
      immunization, this vaccine formula induced robust Th1 immune response 
      characterized by cytokine profiles from restimulated splenocytes and specific 
      antibody titer. More importantly, enhanced cytotoxic CD8(+) T cell activation was 
      observed. However, it did not reduce the bacteria burden in the lung and spleen 
      from the aerosol Mtb challenge. Taken together, OCNs are highly effective in 
      delivering subunit protein vaccine and induce robust Th1 and CD8(+) T cell 
      response. This vaccine delivery system is suitable for application in settings 
      where cell-mediated immune response is needed.
FAU - Sawutdeechaikul, Pritsana
AU  - Sawutdeechaikul P
AD  - Graduate Program in Microbiology and Microbial Technology, Department of 
      Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, 
      Thailand.
AD  - Center of Excellence in Immune-mediated Diseases, Chulalongkorn University, 
      Bangkok 10330, Thailand.
FAU - Cia, Felipe
AU  - Cia F
AD  - Department of Immunology and Infection, London School of Hygiene and Tropical 
      Medicine, Keppel Street, London, WC1E 7HT, UK.
FAU - Bancroft, Gregory
AU  - Bancroft G
AD  - Department of Immunology and Infection, London School of Hygiene and Tropical 
      Medicine, Keppel Street, London, WC1E 7HT, UK.
FAU - Wanichwecharungruang, Supason
AU  - Wanichwecharungruang S
AD  - Center of Excellence in Materials and Bio-Interfaces, Chulalongkorn University 
      and Department of Chemistry, Faculty of Science, Chulalongkorn University, 
      Bangkok 10330, Thailand.
FAU - Sittplangkoon, Chutamath
AU  - Sittplangkoon C
AD  - Center of Excellence in Immune-mediated Diseases, Chulalongkorn University, 
      Bangkok 10330, Thailand.
AD  - Graduate Program in Biotechnology, Faculty of Science, Chulalongkorn University, 
      Bangkok, Thailand 10330.
FAU - Palaga, Tanapat
AU  - Palaga T
AD  - Graduate Program in Microbiology and Microbial Technology, Department of 
      Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, 
      Thailand.
AD  - Center of Excellence in Immune-mediated Diseases, Chulalongkorn University, 
      Bangkok 10330, Thailand.
AD  - Department of Immunology and Infection, London School of Hygiene and Tropical 
      Medicine, Keppel Street, London, WC1E 7HT, UK.
AD  - Center of Excellence in Materials and Bio-Interfaces, Chulalongkorn University 
      and Department of Chemistry, Faculty of Science, Chulalongkorn University, 
      Bangkok 10330, Thailand.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 0 (HspX protein, Mycobacterium tuberculosis)
RN  - 0 (Tuberculosis Vaccines)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Vaccines, Synthetic)
RN  - 7440-44-0 (Carbon)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (antigen 85B, Mycobacterium tuberculosis)
SB  - IM
MH  - Acyltransferases/genetics
MH  - Adjuvants, Immunologic
MH  - Administration, Mucosal
MH  - Animals
MH  - Antigens, Bacterial/genetics/immunology
MH  - Bacterial Proteins/genetics/immunology
MH  - Bone Marrow
MH  - Carbon/*chemistry
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects
MH  - Disease Models, Animal
MH  - Drug Delivery Systems/*methods
MH  - Female
MH  - Immunity, Cellular
MH  - Immunization
MH  - Lung/microbiology
MH  - Macrophages/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mycobacterium tuberculosis/pathogenicity
MH  - Nanospheres/*chemistry
MH  - Spleen/microbiology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Th1 Cells/drug effects
MH  - Tuberculosis/*prevention & control
MH  - *Tuberculosis Vaccines
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Vaccines, Subunit/administration & dosage/*chemistry/*immunology
MH  - Vaccines, Synthetic
OTO - NOTNLM
OT  - Carbon nanosphere
OT  - cytotoxic T lymphocyte
OT  - subunit vaccine
OT  - tuberculosis
OT  - vaccine delivery
EDAT- 2019/01/30 06:00
MHDA- 2019/04/27 06:00
CRDT- 2019/01/30 06:00
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/04/27 06:00 [medline]
PHST- 2019/01/30 06:00 [entrez]
AID - 10.4014/jmb.1809.09049 [pii]
AID - 10.4014/jmb.1809.09049 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2019 Mar 28;29(3):489-499. doi: 10.4014/jmb.1809.09049.