PMID- 30691473
OWN - NLM
STAT- MEDLINE
DCOM- 20190429
LR  - 20201209
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 16
IP  - 1
DP  - 2019 Jan 28
TI  - Involvement of PRRSV NSP3 and NSP5 in the autophagy process.
PG  - 13
LID - 10.1186/s12985-019-1116-x [doi]
LID - 13
AB  - BACKGROUND: Autophagy is an essential process in eukaryotic cells in which 
      autophagosomes form to deliver cellular organelles and long-lived proteins to 
      lysosomes for degradation. Many studies have recently identified the regulatory 
      mechanisms involved in the interaction between viral infection and autophagy. 
      METHODS: LC3 turnover and the proteins in the endoplasmic reticulum (ER) stress 
      pathway were investigated using western blot analysis. The formation and 
      degradation of autophagosomes were detected using immunofluorescence staining. 
      RESULTS: Autophagy was activated by porcine reproductive and respiratory syndrome 
      virus (PRRSV) NSP3, NSP5 and NSP9, which are two transmembrane proteins and an 
      RNA-dependent RNA polymerase, respectively. The formation of autophagosomes was 
      induced by NSP3 and NSP5 and developed from the ER; the fusion of these 
      autophagosomes with lysosomes was limited. Although NSP3 and NSP5 are ER 
      transmembrane proteins, these proteins did not activate the ER stress signaling 
      pathways. In addition, the cytoplasmic domain of NSP3 plays a pivotal role in 
      activating autophagy. CONCLUSIONS: The data presented in this study reveal an 
      important relationship between PRRSV NSPs and autophagy and provide new insights 
      that improve our understanding of the involvement of PRRSV NSPs in the autophagy 
      process.
FAU - Zhang, Wei
AU  - Zhang W
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China.
FAU - Chen, Keren
AU  - Chen K
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China.
FAU - Guo, Yang
AU  - Guo Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China.
FAU - Chen, Yaosheng
AU  - Chen Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China.
FAU - Liu, Xiaohong
AU  - Liu X
AUID- ORCID: 0000-0003-3733-4605
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China. xhliu@163.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190128
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (nsp3 protein, alphavirus)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Animals
MH  - Autophagosomes/*virology
MH  - *Autophagy
MH  - Cell Line
MH  - Endoplasmic Reticulum Stress
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - RNA, Viral/genetics
MH  - RNA-Dependent RNA Polymerase/genetics/metabolism
MH  - Signal Transduction
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - Virus Replication
PMC - PMC6350329
OTO - NOTNLM
OT  - Autophagosomes
OT  - Cytoplasmic domain
OT  - Endoplasmic reticulum
OT  - NSP3 and NSP5
OT  - Porcine reproductive and respiratory syndrome virus
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2019/01/30 06:00
MHDA- 2019/04/30 06:00
PMCR- 2019/01/28
CRDT- 2019/01/30 06:00
PHST- 2018/07/25 00:00 [received]
PHST- 2019/01/01 00:00 [accepted]
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/04/30 06:00 [medline]
PHST- 2019/01/28 00:00 [pmc-release]
AID - 10.1186/s12985-019-1116-x [pii]
AID - 1116 [pii]
AID - 10.1186/s12985-019-1116-x [doi]
PST - epublish
SO  - Virol J. 2019 Jan 28;16(1):13. doi: 10.1186/s12985-019-1116-x.