PMID- 30692826
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220401
IS  - 1016-3190 (Print)
IS  - 2223-8956 (Electronic)
VI  - 31
IP  - 1
DP  - 2019 Jan-Mar
TI  - Transplanting human umbilical cord mesenchymal stem cells and hyaluronate 
      hydrogel repairs cartilage of osteoarthritis in the minipig model.
PG  - 11-19
LID - 10.4103/tcmj.tcmj_87_18 [doi]
AB  - OBJECTIVES: Osteoarthritis (OA) is a chronic disease of degenerative joints. 
      Mesenchymal stem cells (MSCs) have been used for cartilage regeneration in OA. We 
      investigated the therapeutic potential of human umbilical cord-derived MSCs 
      (HUCMSCs) with hyaluronic acid (HA) hydrogel transplanted into a porcine OA 
      preclinical model. MATERIALS AND METHODS: The HUCMSCs were characterized with 
      respect to morphology, surface markers, and differentiation capabilities. 
      Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used 
      to examine gene expressions in a HUCMSC-HA coculture. Two healthy female minipigs 
      weighing 30-40 kg and aged approximately 4 months were used in this large animal 
      study. A full-thickness chondral injury was created in the trochlear groove of 
      each of the pig's rear knees. After 3 weeks, a second osteochondral defect was 
      created. Then, 1.5 mL of a HUCMSC (5 x 10(6) cells) and HA composite (4%) was 
      transplanted into the chondral-injured area in the right knee of each pig. Using 
      the same surgical process, an osteochondral defect (untreated) was created in the 
      left knee as a control. The pigs were sacrificed 12 weeks after transplantation. 
      Macroscopic and microscopic histologies, qRT-PCR, and immunostaining evaluated 
      the degree of chondral degradation. RESULTS: The HUCMSCs exhibited typical MSC 
      characteristics, including spindle morphology, expression of surface markers 
      (positive for CD29, CD4, CD73, CD90, and human leukocyte antigen [HLA]-ABC; 
      negative for CD34, CD45, and HLA-DR), and multipotent differentiation 
      (adipogenesis, osteogenesis, and chondrogenesis). More extensive proliferation of 
      HUCMSCs was noted with 4% and 25% of HA than without HA. Expression of COL2A1 and 
      aggrecan in the HUCMSC-derived chondrocytes was increased when HA was included. 
      The treated knees showed significant gross and histological improvements in 
      hyaline cartilage regeneration when compared to the control knees. The 
      International Cartilage Repair Society histological score was higher for the 
      treated knees than the control knees. CONCLUSION: Our findings suggest that 
      cartilage regeneration using a mixture of HUCMSCs and HA in a large animal model 
      may be an effective treatment for OA, and this study is a stepping stone toward 
      the future clinical trials.
FAU - Wu, Kun-Chi
AU  - Wu KC
AD  - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation and Tzu Chi University, Hualien, Taiwan.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AD  - Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation and Tzu Chi University, Hualien, Taiwan.
FAU - Liu, Hwan-Wun
AU  - Liu HW
AD  - Department of Occupational Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation and Tzu Chi University, Hualien, Taiwan.
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
AD  - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu 
      Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan.
AD  - Department of Research, Stem Cell Laboratory, Hualien Tzu Chi Hospital, Buddhist 
      Tzu Chi Medical Foundation, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Ci Ji Yi Xue Za Zhi
JT  - Ci ji yi xue za zhi = Tzu-chi medical journal
JID - 9514171
PMC - PMC6334562
OTO - NOTNLM
OT  - Cartilage
OT  - Human umbilical cord
OT  - Hyaluronate
OT  - Mesenchymal stem cells
OT  - Regeneration
COIS- There are no conflicts of interest.
EDAT- 2019/01/30 06:00
MHDA- 2019/01/30 06:01
PMCR- 2019/01/01
CRDT- 2019/01/30 06:00
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/01/30 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - TCMJ-31-11 [pii]
AID - 10.4103/tcmj.tcmj_87_18 [doi]
PST - ppublish
SO  - Ci Ji Yi Xue Za Zhi. 2019 Jan-Mar;31(1):11-19. doi: 10.4103/tcmj.tcmj_87_18.