PMID- 30692975
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Comparative Evaluation of Microbiota Engraftment Following Fecal Microbiota 
      Transfer in Mice Models: Age, Kinetic and Microbial Status Matter.
PG  - 3289
LID - 10.3389/fmicb.2018.03289 [doi]
LID - 3289
AB  - The intestinal microbiota and its functions are intricately interwoven with host 
      physiology. Colonizing rodents with donor microbiota provides insights into 
      host-microbiota interactions characterization and the understanding of disease 
      physiopathology. However, a better assessment of inoculation methods and 
      recipient mouse models is needed. Here, we compare the engraftment at short and 
      long term of genetically obese mice microbiota in germ-free (GF) mice and 
      juvenile and adult specific pathogen free (SPF) mice. We also tested the effects 
      of initial microbiota depletion before microbiota transfer. In the present work, 
      donor microbiota engraftment was better in juvenile SPF mice than in adult SPF 
      mice. In juvenile mice, initial microbiota depletion using laxatives or 
      antibiotics improved donor microbiota engraftment 9 weeks but not 3 weeks after 
      microbiota transfer. Microbiota-depleted juvenile mice performed better than GF 
      mice 3 weeks after the microbiota transfer. However, 9 weeks after transfer, 
      colonized GF mice microbiota had the lowest Unifrac distance to the donor 
      microbiota. Colonized GF mice were also characterized by a chronic alteration in 
      intestinal absorptive function. With these collective results, we show that the 
      use of juvenile mice subjected to initial microbiota depletion constitutes a 
      valid alternative to GF mice in microbiota transfer studies.
FAU - Le Roy, Tiphaine
AU  - Le Roy T
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
FAU - Debedat, Jean
AU  - Debedat J
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
FAU - Marquet, Florian
AU  - Marquet F
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
FAU - Da-Cunha, Carla
AU  - Da-Cunha C
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
FAU - Ichou, Farid
AU  - Ichou F
AD  - ICANalytics Facility Core, Institut de Cardiometabolisme et Nutrition (ICAN), 
      Paris, France.
FAU - Guerre-Millo, Michele
AU  - Guerre-Millo M
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
FAU - Kapel, Nathalie
AU  - Kapel N
AD  - Department of Functional Coprology, Pitie-Salpetriere Hospital, Assistance 
      Publique-Hopitaux de Paris, Paris, France.
FAU - Aron-Wisnewsky, Judith
AU  - Aron-Wisnewsky J
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
AD  - Department of Nutrition, CRNH Ile de France, Pitie-Salpetriere Hospital, 
      Assistance Publique Hopitaux de Paris, Paris, France.
FAU - Clement, Karine
AU  - Clement K
AD  - NutriOmics Team, INSERM, ICAN, Sorbonne Universite, Paris, France.
AD  - Department of Nutrition, CRNH Ile de France, Pitie-Salpetriere Hospital, 
      Assistance Publique Hopitaux de Paris, Paris, France.
LA  - eng
PT  - Journal Article
DEP - 20190114
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6339881
OTO - NOTNLM
OT  - FMT fecal microbiota transplant
OT  - SPF
OT  - adult
OT  - antibiotics
OT  - germ-free animals
OT  - microbiota
OT  - polyethylene glycol
OT  - weaning (severage)
EDAT- 2019/01/30 06:00
MHDA- 2019/01/30 06:01
PMCR- 2019/01/14
CRDT- 2019/01/30 06:00
PHST- 2018/08/06 00:00 [received]
PHST- 2018/12/18 00:00 [accepted]
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/01/30 06:01 [medline]
PHST- 2019/01/14 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.03289 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Jan 14;9:3289. doi: 10.3389/fmicb.2018.03289. eCollection 
      2018.