PMID- 30694456
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20200225
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 2
DP  - 2019 Apr
TI  - Episomal minicircles persist in periods of transcriptional inactivity and can be 
      transmitted through somatic cell nuclear transfer into bovine embryos.
PG  - 1737-1746
LID - 10.1007/s11033-019-04624-x [doi]
AB  - Episomal plasmids based on a scaffold/matrix attachment region (S/MAR) are 
      extrachromosomal DNA entities that replicate once per cell cycle and are stably 
      maintained in cells or tissue. We generated minicircles, episomal plasmids devoid 
      of bacterial sequences, and show that they are stably transmitted in clonal 
      primary bovine fibroblasts without selection pressure over more than two months. 
      Total DNA, plasmid extraction and fluorescence in situ hybridization (FISH) 
      analyses suggest that the minicircles remained episomal and were not integrated 
      into the genome. Minicircles survived extended periods in serum-starved cells, 
      which indicates that ongoing transcription in non-proliferating cells is not 
      necessary for the maintenance of S/MAR-episomes. To test whether minicircles 
      endure the process of somatic cell nuclear transfer (SCNT), we used cell-cycle 
      synchronized, serum-starved, minicircle-containing cells. Analysis of cells 
      outgrown from SCNT-derived blastocysts shows that the minicircles are maintained 
      through SCNT and early embryonic development, which raises the prospect of using 
      cell lines with episomal minicircles for the generation of transgenic animals.
FAU - Wagner, Stefan
AU  - Wagner S
AUID- ORCID: 0000-0003-4874-9304
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand. 
      stefan.wagner@abdn.ac.uk.
AD  - Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, UK. 
      stefan.wagner@abdn.ac.uk.
FAU - McCracken, Judi
AU  - McCracken J
AUID- ORCID: 0000-0003-4709-6479
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
FAU - Bruszies, Sabine
AU  - Bruszies S
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
FAU - Broadhurst, Ric
AU  - Broadhurst R
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
FAU - Wells, David N
AU  - Wells DN
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
FAU - Oback, Bjorn
AU  - Oback B
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
FAU - Bode, Jurgen
AU  - Bode J
AD  - Hannover Medical School (MHH), Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.
FAU - Laible, Gotz
AU  - Laible G
AD  - AgResearch Limited, Ruakura Research Centre, Hamilton, 3240, New Zealand.
LA  - eng
GR  - 123/Ministry of Business, Innovation and Employment/
PT  - Journal Article
DEP - 20190129
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA, Circular)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/genetics
MH  - Blastocyst
MH  - Cattle
MH  - DNA, Circular/genetics/*physiology
MH  - Genetic Vectors/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Nuclear Transfer Techniques/veterinary
MH  - Plasmids/*genetics/*physiology
OTO - NOTNLM
OT  - Episome
OT  - Minicircle
OT  - Plasmid
OT  - Scaffold/matrix attachment region
OT  - Transgenic animals
EDAT- 2019/01/30 06:00
MHDA- 2019/09/10 06:00
CRDT- 2019/01/30 06:00
PHST- 2018/09/26 00:00 [received]
PHST- 2019/01/18 00:00 [accepted]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2019/01/30 06:00 [entrez]
AID - 10.1007/s11033-019-04624-x [pii]
AID - 10.1007/s11033-019-04624-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Apr;46(2):1737-1746. doi: 10.1007/s11033-019-04624-x. Epub 
      2019 Jan 29.