PMID- 30694457
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20200225
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 2
DP  - 2019 Apr
TI  - Selection of suitable reference genes for quantitative real-time PCR gene 
      expression analysis in Mulberry (Morus alba L.) under different abiotic stresses.
PG  - 1809-1817
LID - 10.1007/s11033-019-04631-y [doi]
AB  - Mulberry (Morus alba L.) is the sole food source for the mulberry silkworm, 
      Bombyx mori and therefore important for sericulture industry. Different abiotic 
      stress conditions like drought, salt, heat and cold stress adversely affect the 
      productivity and quality of mulberry leaves. Quantitative real time PCR (qPCR) is 
      a reliable and widely used method to identify abiotic stress responsive genes and 
      molecular mechanism in different plant species. Selection of suitable reference 
      genes is important requirement for normalizing the expression of genes through 
      qRT-PCR study. In the present study, we have selected eight candidate reference 
      genes in mulberry for analyzing their expression stability in different abiotic 
      stress treatments including drought, salt, heat and cold stresses. The expression 
      stability of these reference genes was determined using geNorm, NormFinder and 
      RefFinder statistical algorithms. The results showed that Ubiquitin and protein 
      phosphatase 2A regulatory subunit A (PP2A) were the most stable genes across all 
      the treatment samples. However, analysis of individual stresses revealed 
      different expression profiles and stability of reference genes. Actin3 and PP2A 
      were most stable in drought and salt conditions respectively. RPL3 most preferred 
      in heat stress and Ubiquitin was most stable in cold stress. We propose the 
      ubiquitin and PP2A are the preferred reference genes for normalization of gene 
      expression data from abiotic stresses. In addition, Actin3 are preferred for 
      drought stress, PP2A for salt stress, RPL3 for heat stress and Ubiquitin for cold 
      stress studies.
FAU - Shukla, Pawan
AU  - Shukla P
AUID- ORCID: 0000-0002-5401-7188
AD  - Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, 
      Gallandar, Pampore -192 121, Jammu and Kashmir, Srinagar, India. 
      shklpwn@gmail.com.
FAU - Reddy, Ramesha A
AU  - Reddy RA
AD  - Seri-biotech Research Laboratory (SBRL), Carmelram Post, Kodathi, Bangalore, 
      560035, India.
FAU - Ponnuvel, Kangayam M
AU  - Ponnuvel KM
AD  - Seri-biotech Research Laboratory (SBRL), Carmelram Post, Kodathi, Bangalore, 
      560035, India.
FAU - Rohela, Gulab Khan
AU  - Rohela GK
AD  - Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, 
      Gallandar, Pampore -192 121, Jammu and Kashmir, Srinagar, India.
FAU - Shabnam, Aftab A
AU  - Shabnam AA
AD  - Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, 
      Gallandar, Pampore -192 121, Jammu and Kashmir, Srinagar, India.
FAU - Ghosh, M K
AU  - Ghosh MK
AD  - Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, 
      Gallandar, Pampore -192 121, Jammu and Kashmir, Srinagar, India.
FAU - Mishra, Rakesh Kumar
AU  - Mishra RK
AD  - Seri-biotech Research Laboratory (SBRL), Carmelram Post, Kodathi, Bangalore, 
      560035, India.
LA  - eng
GR  - PIB-3579/Central Silk Board/
PT  - Journal Article
DEP - 20190129
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Droughts
MH  - Gene Expression/*genetics
MH  - Gene Expression Profiling/methods/standards
MH  - Gene Expression Regulation, Plant/genetics
MH  - Genes, Plant/genetics
MH  - Heat-Shock Response
MH  - Morus/*genetics
MH  - Plant Leaves/metabolism
MH  - Plant Proteins/genetics
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Reference Standards
MH  - Stress, Physiological/*genetics
OTO - NOTNLM
OT  - Abiotic stress
OT  - Cold stress
OT  - Drought stress
OT  - Mulberry
OT  - Reference gene
OT  - Salt stress
EDAT- 2019/01/30 06:00
MHDA- 2019/09/10 06:00
CRDT- 2019/01/30 06:00
PHST- 2018/12/04 00:00 [received]
PHST- 2019/01/19 00:00 [accepted]
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2019/01/30 06:00 [entrez]
AID - 10.1007/s11033-019-04631-y [pii]
AID - 10.1007/s11033-019-04631-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Apr;46(2):1809-1817. doi: 10.1007/s11033-019-04631-y. Epub 
      2019 Jan 29.