PMID- 30694616 OWN - NLM STAT- MEDLINE DCOM- 20200304 LR - 20231006 IS - 2192-2659 (Electronic) IS - 2192-2640 (Print) IS - 2192-2640 (Linking) VI - 8 IP - 4 DP - 2019 Feb TI - A Foreign Body Response-on-a-Chip Platform. PG - e1801425 LID - 10.1002/adhm.201801425 [doi] AB - Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR-on-a-chip (FBROC) for modeling the cascade of events during immune cell response to implants. The platform models the native implant microenvironment where the implants are interfaced directly with surrounding tissues, as well as vasculature with circulating immune cells. The study demonstrates that the release of cytokines such as monocyte chemoattractant protein 1 (MCP-1) from the extracellular matrix (ECM)-like hydrogels in the bottom tissue chamber induces trans-endothelial migration of circulating monocytes in the vascular channel toward the hydrogels, thus mimicking implant-induced inflammation. Data using patient-derived peripheral blood mononuclear cells further reveal inter-patient differences in FBR, highlighting the potential of this platform for monitoring FBR in a personalized manner. The prototype FBROC platform provides an enabling strategy to interrogate FBR on various implants, including biomaterials and engineered tissue constructs, in a physiologically relevant and individual-specific manner. CI - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. FAU - Sharifi, Fatemeh AU - Sharifi F AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. AD - School of Mechanical Engineering, Sharif University of Technology, Tehran, 11155-8639, Iran. FAU - Htwe, Su Su AU - Htwe SS AD - Immunology and Immuno-bioengineering Group, School of Life Science, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, NG7 2RD, UK. FAU - Righi, Martina AU - Righi M AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Liu, Hua AU - Liu H AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Pietralunga, Anna AU - Pietralunga A AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Yesil-Celiktas, Ozlem AU - Yesil-Celiktas O AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. AD - Department of Bioengineering, Faculty of Engineering Ege University, Bornova, 35100, Izmir, Turkey. FAU - Maharjan, Sushila AU - Maharjan S AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Cha, Byung-Hyun AU - Cha BH AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Shin, Su Ryon AU - Shin SR AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. FAU - Dokmeci, Mehmet Remzi AU - Dokmeci MR AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. AD - The BioRobotics Institute, Sant'Anna School of Advanced Studies, Piaggio, 56025, Italy. AD - Department of Radiology, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA. AD - Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA, 90095, USA. AD - California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, 90095, USA. AD - Department of Bioindustrial Technologies, Konkuk University, Seoul, 05029, Republic of Korea. FAU - Vrana, Nihal Engin AU - Vrana NE AD - Biomateriaux et Bioingenierie, Institut National de la Sante et de la Recherche Medicale (INSERM), 67085, Strasbourg, France. AD - Protip Medical, 67000, Strasbourg, France. AD - Federation de Medecine Translationnelle de Strasbourg, Federation des Materiaux et Nanoscience d'Alsace (FMNA), Faculte de Chirurgie Dentaire, Universite de Strasbourg, 67000, Strasbourg, France. FAU - Ghaemmaghami, Amir M AU - Ghaemmaghami AM AD - Immunology and Immuno-bioengineering Group, School of Life Science, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, NG7 2RD, UK. FAU - Khademhosseini, Ali AU - Khademhosseini A AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. AD - The BioRobotics Institute, Sant'Anna School of Advanced Studies, Piaggio, 56025, Italy. AD - Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, 310012, P. R. China. AD - Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, 310012, P. R. China. AD - Research Institute for Bioscience and Biotechnology, Lalitpur, 44600, Nepal. AD - Center for Minimally Invasive Therapeutics (C-MIT), University of California-Los Angeles, Los Angeles, CA, 90095, USA. AD - Department of Radiology, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA. AD - Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA, 90095, USA. AD - California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, 90095, USA. FAU - Zhang, Yu Shrike AU - Zhang YS AUID- ORCID: 0000-0002-0045-0808 AD - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. LA - eng GR - New England Anti-Vivisection Society/International GR - R01 AR066193/AR/NIAMS NIH HHS/United States GR - R21 EB026175/EB/NIBIB NIH HHS/United States GR - R01 DE021468/DE/NIDCR NIH HHS/United States GR - R01 AR057837/AR/NIAMS NIH HHS/United States GR - R01 AR074234/AR/NIAMS NIH HHS/United States GR - R21 EB026824/EB/NIBIB NIH HHS/United States GR - K99 CA201603/CA/NCI NIH HHS/United States GR - R21 EB025270/EB/NIBIB NIH HHS/United States GR - 760921/European Union's FP7 and Horizon 2020 research and innovation programme/International GR - R21 AR068258/AR/NIAMS NIH HHS/United States GR - R21 EB022403/EB/NIBIB NIH HHS/United States GR - 602694/European Union's FP7 and Horizon 2020 research and innovation programme/International GR - R00 CA201603/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20190129 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 RN - 0 (Hydrogels) SB - IM MH - *Foreign-Body Reaction/immunology/pathology MH - *Human Umbilical Vein Endothelial Cells/immunology/pathology MH - Humans MH - Hydrogels/chemistry MH - *Lab-On-A-Chip Devices MH - *Microfluidic Analytical Techniques MH - *Monocytes/immunology/pathology MH - THP-1 Cells MH - Transendothelial and Transepithelial Migration/*immunology PMC - PMC6398437 MID - NIHMS1014437 OTO - NOTNLM OT - biomaterials OT - foreign body responses OT - immune responses OT - implants OT - organs-on-a-chip COIS- Conflict of Interest The authors declare no conflict of interest. EDAT- 2019/01/30 06:00 MHDA- 2020/03/05 06:00 PMCR- 2020/02/01 CRDT- 2019/01/30 06:00 PHST- 2018/11/06 00:00 [received] PHST- 2018/12/17 00:00 [revised] PHST- 2019/01/30 06:00 [pubmed] PHST- 2020/03/05 06:00 [medline] PHST- 2019/01/30 06:00 [entrez] PHST- 2020/02/01 00:00 [pmc-release] AID - 10.1002/adhm.201801425 [doi] PST - ppublish SO - Adv Healthc Mater. 2019 Feb;8(4):e1801425. doi: 10.1002/adhm.201801425. Epub 2019 Jan 29.