PMID- 30694908
OWN - NLM
STAT- MEDLINE
DCOM- 20191129
LR  - 20211204
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 30
IP  - 4
DP  - 2019 Mar 6
TI  - Leonurine promotes neurite outgrowth and neurotrophic activity by modulating the 
      GR/SGK1 signaling pathway in cultured PC12 cells.
PG  - 247-254
LID - 10.1097/WNR.0000000000001180 [doi]
AB  - Depression is a common psychiatric disorder that affects almost 10% of children 
      and adolescents worldwide. Numerous synthetic chemical antidepressants used to 
      treat depression have adverse side effects. Therefore, new therapeutic approaches 
      for depression treatment are urgently needed. Leonurus cardiaca has recently been 
      shown to be effective for the treatment of nervous system diseases such as 
      depression, but its mechanism is not clear. In this study, we aimed to reveal the 
      mechanism underlying leonurine's antidepressant activity. Leonurine was used to 
      treat corticosterone-induced PC12 cells to examine its effect on neurite 
      outgrowth and neurotrophic factors after treatment with the inhibitor of 
      glucocorticoid receptor (GR) and serum-inducible and glucocorticoid-inducible 
      kinase 1 (SGK1). Methyl thiazolyl tetrazolium assays were used to evaluate the 
      viability of cells. High content analysis was used to detect cell area, total 
      neurite length, maximum neurite length, and expression of GR, SGK1, brain-derived 
      neurotrophic factor (BDNF), neurotrophic factor-3 (NT-3), and B-cell lymphoma-2 
      (BCL-2). The results showed that leonurine increased cell viability in a 
      concentration-dependent manner, with the maximal prosurvival effect at 60 muM. 
      Leonurine increased cell area, total neurite length, and maximum neurite length 
      of corticosterone-induced PC12 cells, increased the expression of GR, BDNF, NT-3, 
      and BCL-2, and decreased the expression of SGK1. After treatment with GR 
      inhibitor RU486, the expressions of GR, BDNF, NT-3, and BCL-2 were significantly 
      decreased and SGK1 was increased. In contrast, treatment with GSK650394 had the 
      opposite effect of RU486. Our data indicate that leonurine promotes neurite 
      outgrowth and neurotrophic activity in cultured PC12 cells, and its potential 
      mechanism may involve the GR/SGK1 signaling pathway.
FAU - Meng, Pan
AU  - Meng P
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
FAU - Zhu, Qing
AU  - Zhu Q
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
AD  - The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Yang, Hui
AU  - Yang H
AD  - The First Affiliated Hospital, Hunan University of Chinese Medicine.
FAU - Liu, Dan
AU  - Liu D
AD  - The First Affiliated Hospital, Hunan University of Chinese Medicine.
FAU - Lin, Xiaoyuan
AU  - Lin X
AD  - The First Affiliated Hospital, Hunan University of Chinese Medicine.
FAU - Liu, Jian
AU  - Liu J
AD  - The First Affiliated Hospital, Hunan University of Chinese Medicine.
FAU - Fan, Jingying
AU  - Fan J
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
FAU - Liu, Xiaodan
AU  - Liu X
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
FAU - Su, Wei
AU  - Su W
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
FAU - Liu, Lin
AU  - Liu L
AD  - The First Affiliated Hospital, Hunan University of Chinese Medicine.
FAU - Wang, Yuhong
AU  - Wang Y
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
FAU - Cai, Xiong
AU  - Cai X
AD  - Institute of Innovation and Applied Research in Chinese Medicine.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Antidepressive Agents)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 09Q5W34QDA (leonurine)
RN  - 632XD903SP (Gallic Acid)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (serum-glucocorticoid regulated kinase)
SB  - IM
MH  - Animals
MH  - Antidepressive Agents/*pharmacology
MH  - Cell Survival/drug effects
MH  - Gallic Acid/*analogs & derivatives/pharmacology
MH  - Immediate-Early Proteins/drug effects/metabolism
MH  - Neuronal Outgrowth/*drug effects
MH  - PC12 Cells
MH  - Protein Serine-Threonine Kinases/drug effects/metabolism
MH  - Rats
MH  - Receptors, Glucocorticoid/drug effects/metabolism
MH  - Signal Transduction/*drug effects
PMC - PMC6392205
EDAT- 2019/01/30 06:00
MHDA- 2019/11/30 06:00
PMCR- 2019/02/27
CRDT- 2019/01/30 06:00
PHST- 2019/01/30 06:00 [pubmed]
PHST- 2019/11/30 06:00 [medline]
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/02/27 00:00 [pmc-release]
AID - 10.1097/WNR.0000000000001180 [doi]
PST - ppublish
SO  - Neuroreport. 2019 Mar 6;30(4):247-254. doi: 10.1097/WNR.0000000000001180.