PMID- 30695863
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20190401
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 48
IP  - 2
DP  - 2019 Feb 8
TI  - [Combination of environmental friendly reagent and ultrasonic assisted rapid 
      processing for protein and molecular detection in tumor biopsy specimens].
PG  - 116-119
LID - 10.3760/cma.j.issn.0529-5807.2019.02.008 [doi]
AB  - Objective: To investigate the impact of ultrasonic assisted rapid processing 
      technique combined with the environment friendly reagent (which can be utilized 
      in fixing,dehydrating and clearing) on processing tumor biopsy specimens and the 
      subsequent target detection. Methods: Postoperative tissue samples of 56 cases of 
      breast cancer, colorectal cancer, lung cancer, stomach cancer, liver mass, 
      bladder mass, uterus mass were obtained at the National Cancer Center, Cancer 
      Hospital, Chinese Academy of Medical Sciences from February to April, 2017. Three 
      specimens ranging in size from 1 to 3 mm were collected from each sample, and 
      were separated into control group (traditional tissue-processing method); 
      experiment group 1 (3.7% neutral buffered formaldehyde fixation, composite 
      environment friendly reagent and ultrasonic assisted rapid processing) and 
      experimental group 2 (composite environment friendly reagent direct fixation, 
      higher temperature and longer time for tissue processing). Two pathologists 
      blinded to the experimental groups scored totally the nuclear, cytoplasmic, and 
      membrane staining of 43 cases of immunohistochemistry (IHC), four HER2 
      fluorescence in situ hybridization (FISH), 20 extracted DNA quality and four EGFR 
      gene mutation detection in lung adenocarcinoma; the results were compared with 
      the control group. Results: There was no difference in the IHC staining, HER2 
      FISH, the DNA quality, and EGFR genetic results between experimental group 1 and 
      control group. For experiment group 2, comparing results of IHC staining, HER2 
      FISH and the quality of DNA, there was no obvious difference from control group 
      and experiment group 1, but might show an increase in the background of IHC 
      staining. The difference between the treatment temperature and time in the 
      experimental group 2 did not affect the results of the gene mutation detection. 
      Conclusions: Environment freindly reagent and ultrasonic assisted rapid 
      processing equipment could be used for rapid processing and diagnosis for tumor 
      biopsies. Using complex environment-friendly reagents supplement fixation, higher 
      treatment temperature and longer treatment time do not significantly affect the 
      IHC, FISH and molecular detection accuracy.
FAU - Zheng, B
AU  - Zheng B
AD  - Department of Pathology, National Cancer Center, Cancer Hospital, Chinese Academy 
      of Medical Sciences, Beijing 100021, China.
FAU - Wang, P J
AU  - Wang PJ
FAU - Xue, L Y
AU  - Xue LY
FAU - Liu, X Y
AU  - Liu XY
FAU - Guo, L
AU  - Guo L
FAU - Ying, J M
AU  - Ying JM
LA  - chi
GR  - LC2016B04/China Cancer Foundation "Beijing Hope Marathon" Special Fund Youth 
      Project/
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Fixatives)
RN  - 0 (Indicators and Reagents)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biopsy/*methods
MH  - DNA Mutational Analysis/methods
MH  - DNA, Neoplasm/analysis
MH  - Desiccation/*methods
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - *Fixatives
MH  - Formaldehyde
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - *Indicators and Reagents
MH  - Male
MH  - Mutation
MH  - Neoplasms/*genetics/*pathology
MH  - Receptor, ErbB-2/analysis
MH  - Temperature
MH  - Time Factors
MH  - Ultrasonics
OTO - NOTNLM
OT  - Biopsy, needle
OT  - DNA mutational analysis
OT  - Immunohistochemistry
OT  - In situ hybridization, fluorescence
OT  - Tissue fixation
EDAT- 2019/01/31 06:00
MHDA- 2019/04/02 06:00
CRDT- 2019/01/30 06:00
PHST- 2019/01/30 06:00 [entrez]
PHST- 2019/01/31 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2019.02.008 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2019 Feb 8;48(2):116-119. doi: 
      10.3760/cma.j.issn.0529-5807.2019.02.008.