PMID- 30697843
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20191217
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 45
IP  - 3
DP  - 2019 May
TI  - Attenuated levels of ghrelin in synovial fluid is related to the disease severity 
      of ankle post-traumatic osteoarthritis.
PG  - 463-470
LID - 10.1002/biof.1490 [doi]
AB  - Post-traumatic osteoarthritis (PTOA) of ankle joints results in pain and reduced 
      joint function. Ghrelin, a 28-amino-acid polypeptide, has been previously 
      identified as the first cognate natural ligand that binds to the growth hormone 
      secretagogue receptor. In the present study, ghrelin has been validated to exert 
      cartilage-protective and anti-inflammatory effects. The current study was aimed 
      at investigating the potential role of the levels of serum and synovial fluid 
      (SF) ghrelin on the severity of disease in patients suffering from ankle PTOA. 
      Ninety-seven patients with ankle osteoarthritis who received an arthroscopical 
      examination and debridement or replacement of the ankle joint were included in 
      the study cohort. Meanwhile, 95 healthy individuals (whose age and sex were 
      matched) who received periodic body checkups were enrolled as healthy controls. 
      Enzyme-linked immunosorbent assay (ELISA) was used to analyze the ghrelin levels 
      in serum and SF. SF was also probed for cartilage degradation enzyme matrix 
      metalloproteinases-3 (MMP-3) and tumor necrosis factor alpha (TNF-alpha), which is a 
      known pro-inflammatory cytokine. The clinical evaluation was carried out using 
      the American Orthopedic Foot and Ankle Society (AOFAS) ankle-hindfoot rating 
      scale and visual analogue scale (VAS). The radiographic severity was evaluated 
      using the modified Kellgren-Lawrence (K-L) grading system. We scored for the 
      modified Mankin's score to depict histopathological changes due to cartilage 
      lesions. The diagnostic relevance of the ghrelin concentrations in the prediction 
      of the radiographic grading (in comparison with MMP-3 and TNF-alpha) was evaluated by 
      calculating the area under the curve of the receiver operating characteristic 
      (ROC) curve. The serum abundance of ghrelin was not significantly altered between 
      ankle PTOA patients and healthy controls. SF ghrelin was negatively correlated 
      with radiographic progression determined by modified ankle K-L grades. In 
      addition SF ghrelin concentrations were negatively related to VAS scores, and 
      positively associated with AOFAS ankle-hindfoot rating. Moreover, SF ghrelin was 
      inversely proportional to the expressions of MMP-3 and TNF-alpha. ROC analysis curve 
      demonstrated that ghrelin serves as a favorable marker for the diagnosis of 
      radiographic severity by modified ankle K-L grade. The ghrelin concentration in 
      SF is negatively proportional to disease progression in patients suffering from 
      ankle PTOA. Local administration of ghrelin may function as a decent adjuvant 
      therapy to delay the progress of ankle PTOA. (c) 2019 BioFactors, 45(3):463-470, 
      2019.
CI  - (c) 2019 International Union of Biochemistry and Molecular Biology.
FAU - Zou, Yu-Cong
AU  - Zou YC
AD  - Department of Rehabilitation Medicine, The Third Affiliated Hospital, Southern 
      Medical University, Guangzhou, Guangdong Province, China.
FAU - Li, Hai-Hong
AU  - Li HH
AD  - Department of Rehabilitation Medicine, The Third Affiliated Hospital, Southern 
      Medical University, Guangzhou, Guangdong Province, China.
FAU - Yang, Guang-Gang
AU  - Yang GG
AD  - Department of Orthopedics, Guangzhou Orthopedics Hospital, Guangzhou, Guangdong 
      Province, China.
FAU - Yin, Hai-Dong
AU  - Yin HD
AD  - Shunde Hospital of Southern Medical University, Foshan, China.
FAU - Cai, Dao-Zhang
AU  - Cai DZ
AD  - Department of Joint Surgery, The Third Affiliated Hospital, Southern Medical 
      University, Guangzhou, Guangdong Province, China.
FAU - Liu, Gang
AU  - Liu G
AUID- ORCID: 0000-0002-6426-3155
AD  - Department of Rehabilitation Medicine, The Third Affiliated Hospital, Southern 
      Medical University, Guangzhou, Guangdong Province, China.
LA  - eng
GR  - 81774382/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190130
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Ghrelin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Ankle Joint/metabolism
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Ghrelin/*blood/*metabolism
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/blood/metabolism
MH  - Middle Aged
MH  - Osteoarthritis/*blood/*metabolism
MH  - Synovial Fluid/*metabolism
MH  - Tumor Necrosis Factor-alpha/blood/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - ankle
OT  - disease severity
OT  - ghrelin
OT  - post-traumatic osteoarthritis
EDAT- 2019/01/31 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/01/31 06:00
PHST- 2018/11/16 00:00 [received]
PHST- 2018/12/18 00:00 [revised]
PHST- 2018/12/19 00:00 [accepted]
PHST- 2019/01/31 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/01/31 06:00 [entrez]
AID - 10.1002/biof.1490 [doi]
PST - ppublish
SO  - Biofactors. 2019 May;45(3):463-470. doi: 10.1002/biof.1490. Epub 2019 Jan 30.