PMID- 30699535
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 99
IP  - 6
DP  - 2015 Jun
TI  - Investigation of the Colletotrichum gloeosporioides Species Complex Causing Peach 
      Anthracnose in South Carolina.
PG  - 797-805
LID - 10.1094/PDIS-10-14-1076-RE [doi]
AB  - Colletotrichum gloeosporioides, C. acutatum, and C. truncatum are causal agents 
      of anthracnose disease of peach in South Carolina, but more recent investigations 
      show that C. gloeosporioides is a species complex that has not yet been 
      investigated among peach isolates. A total of 28 Colletotrichum spp. isolates 
      associated with peach fruit anthracnose were collected in 2012 from Chesnee (10 
      isolates), McBee (10 isolates), Monetta (2 isolates), and Ridge Spring (6 
      isolates), South Carolina. Morphological characteristics indicated that all 28 
      isolates belonged to the C. gloeosporioides species complex. Phylogenetic 
      analysis of the combined calmodulin (CAL), glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH), and beta-tubulin (TUB2) gene sequences identified two 
      species, C. siamense and C. fructicola. Cultural characteristics such as colony 
      growth rate, shape and size of conidia, and appressoria from representative 
      isolates of the two species largely matched previous descriptions for C. siamense 
      and C. fructicola. Koch's postulates for C. siamense and C. fructicola were 
      fulfilled, confirming pathogenicity of the two species on peach. A new, two-step 
      multiplex PCR assay was developed to facilitate differentiation of the four known 
      Colletotrichum spp. causing anthracnose of peach in South Carolina, including C. 
      acutatum, C. truncatum, C. siamense, and C. fructicola. The first step 
      distinguished C. acutatum from C. truncatum and the two members of the C. 
      gloeosporioides species complex. The second assay distinguished C. siamense from 
      C. fructicola isolates.
FAU - Hu, Meng-Jun
AU  - Hu MJ
AD  - School of Agricultural, Forest and Environmental Sciences, Clemson University, 
      Clemson, SC 29634.
FAU - Grabke, Anja
AU  - Grabke A
AD  - School of Agricultural, Forest and Environmental Sciences, Clemson University, 
      Clemson, SC 29634.
FAU - Schnabel, Guido
AU  - Schnabel G
AD  - School of Agricultural, Forest and Environmental Sciences, Clemson University, 
      Clemson, SC 29634.
LA  - eng
PT  - Journal Article
DEP - 20150515
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
EDAT- 2015/06/01 00:00
MHDA- 2015/06/01 00:01
CRDT- 2019/02/01 06:00
PHST- 2019/02/01 06:00 [entrez]
PHST- 2015/06/01 00:00 [pubmed]
PHST- 2015/06/01 00:01 [medline]
AID - 10.1094/PDIS-10-14-1076-RE [doi]
PST - ppublish
SO  - Plant Dis. 2015 Jun;99(6):797-805. doi: 10.1094/PDIS-10-14-1076-RE. Epub 2015 May 
      15.