PMID- 30700035
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20211204
IS  - 2073-4425 (Print)
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 10
IP  - 2
DP  - 2019 Jan 29
TI  - Translation of Human beta-Actin mRNA is Regulated by mTOR Pathway.
LID - 10.3390/genes10020096 [doi]
LID - 96
AB  - The mammalian target of rapamycin (mTOR) kinase is a well-known master regulator 
      of growth-dependent gene expression in higher eukaryotes. Translation regulation 
      is an important function of the mTORC1 pathway that controls the synthesis of 
      many ribosomal proteins and translation factors. Housekeeping genes such as 
      beta-actin (ACTB) are widely used as negative control genes in studies of 
      growth-dependent translation. Here we demonstrate that translation of both 
      endogenous and reporter ACTB mRNA is inhibited in the presence of mTOR kinase 
      inhibitor (Torin1) and under amino acid starvation. Notably, 5'UTR and promoter 
      of ACTB are sufficient for the mTOR-dependent translational response, and the 
      degree of mTOR-sensitivity of ACTB mRNA translation is cell type-dependent.
FAU - Eliseeva, Irina
AU  - Eliseeva I
AD  - Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290 
      Moscow, Russia. yeliseeva@vega.protres.ru.
FAU - Vasilieva, Maria
AU  - Vasilieva M
AD  - Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290 
      Moscow, Russia. mv.bioengineer@gmail.com.
FAU - Ovchinnikov, Lev P
AU  - Ovchinnikov LP
AD  - Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290 
      Moscow, Russia. ovchinn@vega.protres.ru.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190129
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 
      (1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo(h)(1,6)naphthyridin-2(1H)-one)
RN  - 0 (Actins)
RN  - 0 (Naphthyridines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Actins/*genetics/metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Naphthyridines/pharmacology
MH  - PC-3 Cells
MH  - Protein Biosynthesis/drug effects
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/*genetics/metabolism
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
PMC - PMC6410274
OTO - NOTNLM
OT  - 5'UTR
OT  - ACTB
OT  - amino acid starvation
OT  - mRNA
OT  - mTOR
OT  - translation regulation
OT  - beta-actin
COIS- The authors declare no conflicts of interest.
EDAT- 2019/02/01 06:00
MHDA- 2019/02/01 06:01
PMCR- 2019/02/01
CRDT- 2019/02/01 06:00
PHST- 2018/12/09 00:00 [received]
PHST- 2019/01/22 00:00 [revised]
PHST- 2019/01/24 00:00 [accepted]
PHST- 2019/02/01 06:00 [entrez]
PHST- 2019/02/01 06:00 [pubmed]
PHST- 2019/02/01 06:01 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - genes10020096 [pii]
AID - genes-10-00096 [pii]
AID - 10.3390/genes10020096 [doi]
PST - epublish
SO  - Genes (Basel). 2019 Jan 29;10(2):96. doi: 10.3390/genes10020096.