PMID- 30701727
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20210915
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 32
IP  - 9
DP  - 2018 Sep 15
TI  - [Preparation and osteogenic properties of poly ( L-lactic acid)/lecithin porous 
      scaffolds with open pore structure].
PG  - 1123-1130
LID - 10.7507/1002-1892.201804127 [doi]
AB  - OBJECTIVE: To investigate the preparation and osteogenic properties of poly ( 
      L-lactic acid)(PLLA)/lecithin porous scaffolds with open pore structure. METHODS: 
      PLLA/lecithin porous scaffolds with different lecithin contents (0, 5%, 10%, 20%, 
      30%, 40%, 50%) were prepared by thermally induced phase separation (groups A, B, 
      C, D, E, F, and G, respectively). Scanning electron microscopy (SEM) was used to 
      observe the surface morphology of the scaffolds. Wide-angle X-ray diffraction 
      (XRD) and differential scanning calorimetry (DSC) were used to detect the 
      crystallinity of the scaffolds. The water uptake ability of the scaffolds was 
      measured. The cell growth and viability of bone marrow mesenchymal stem cells 
      (BMSCs) of mouse on each scaffold was assessed by cell counting kit 8 (CCK-8) 
      method. The osteogenic differentiation ability of BMSCs on each scaffold was 
      evaluated by alkaline phosphatase (ALP) activity. Finally, a critical-size rat 
      calvarial bone defect model was used to evaluate the osteogenesis of the 
      scaffolds in vivo. Micro-CT was used to reconstruct the three-dimensional model 
      of the defect area, and the bone volume and bone mineral density were 
      quantitatively analyzed. RESULTS: SEM results showed that the lecithin could 
      slightly reduce the pore size; when lecithin content was 50%, platelet-like 
      structure could be observed on the scaffolds. Wide angle XRD and DSC showed that 
      the crystallinity of scaffolds gradually decreased with the increase of lecithin 
      content. The water uptake ability test showed that the hydrophilicity of 
      scaffolds increased with the increase of lecithin content. CCK-8 assay showed 
      that cell activity gradually increased with the increase of culture time. After 7 
      days of culture, the absorbance ( A) value of groups C, D, E, and F were 
      significantly higher than that of groups A, B, and G ( P<0.05), but no 
      significant difference was found among groups C, D, E, and F ( P>0.05). After 14 
      days of osteogenic induction, with the increase of lecithin content, there was a 
      significant difference in ALP activity of each group. The ALP activity in groups 
      D, E, F, and G were significantly higher than that in groups A, B, and C ( 
      P<0.05). In vivo, the results of Micro-CT examination and bone volume and bone 
      mineral density showed that the scaffolds with 30% lecithin had the best 
      repairing effect. CONCLUSION: Prepared by thermally induced phase separation, the 
      cytocompatibility, osteogenic differentiation, and bone repair ability of the 
      PLLA/lecithin porous scaffold is obviously better than that of pure PLLA 
      scaffold. PLLA/lecithin porous scaffold with suitable lecithin content is a 
      promising scaffold material for bone tissue engineering.
FAU - Chen, Si
AU  - Chen S
AD  - Department of Biomedical Engineering, School of Materials Science and 
      Engineering, South China University of Technology, Guangzhou Guangdong, 510641, 
      P.R.China;National Engineering Research Center for Tissue Restoration and 
      Reconstruction, South China University of Technology, Guangzhou Guangdong, 
      510006, P.R.China;Key Laboratory of Biomedical Materials and Engineering of the 
      Ministry of Education, South China University of Technology, Guangzhou Guangdong, 
      510006, P.R.China.
FAU - Du, Chang
AU  - Du C
AD  - Department of Biomedical Engineering, School of Materials Science and 
      Engineering, South China University of Technology, Guangzhou Guangdong, 510641, 
      P.R.China;National Engineering Research Center for Tissue Restoration and 
      Reconstruction, South China University of Technology, Guangzhou Guangdong, 
      510006, P.R.China;Key Laboratory of Biomedical Materials and Engineering of the 
      Ministry of Education, South China University of Technology, Guangzhou Guangdong, 
      510006, P.R.China.duchang@scut.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Lecithins)
RN  - 0 (Polyesters)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - *Lactic Acid/pharmacology
MH  - *Lecithins/pharmacology
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - *Osteogenesis/drug effects
MH  - Polyesters
MH  - Rats
MH  - Tissue Engineering
MH  - Tissue Scaffolds
PMC - PMC8413987
OTO - NOTNLM
OT  - Thermally induced phase separation
OT  - bone tissue engineering
OT  - lecithin
OT  - poly (L-lactic acid)
OT  - porous scaffold
EDAT- 2019/02/01 06:00
MHDA- 2019/04/06 06:00
PMCR- 2018/09/01
CRDT- 2019/02/01 06:00
PHST- 2019/02/01 06:00 [entrez]
PHST- 2019/02/01 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2018/09/01 00:00 [pmc-release]
AID - zgxfcjwkzz-32-9-1123 [pii]
AID - 10.7507/1002-1892.201804127 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Sep 15;32(9):1123-1130. doi: 
      10.7507/1002-1892.201804127.