PMID- 30702035
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20191210
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 138
IP  - 12
DP  - 2018 Dec
TI  - Evaluation of microRNA expression profiling in highly metastatic laryngocarcinoma 
      cells.
PG  - 1105-1111
LID - 10.1080/00016489.2018.1508887 [doi]
AB  - BACKGROUND: Until now, little is known about the role of miRNAs in the invasion 
      and metastasis of Laryngeal squamous cell carcinoma (LSCC). OBJECTIVES: This 
      study aimed to explore the relationship between microRNA and the invasion and 
      metastasis of LSCC. MATERIAL AND METHODS: The highly metastatic laryngocarcinoma 
      cells were obtained from the established animal model with spontaneous lymph node 
      metastasis of LSCC in our previous study. MicroRNA expression profiling and 
      bioinformatic analysis were performed to analyze the microRNA expression changes 
      in the highly metastatic laryngocarcinoma cells and the parental tumor cells 
      (HEP-2). RT-PCR was performed for further validation of the result of microarray. 
      RESULTS: A total of 40 microRNAs were found to be significantly altered in the 
      highly metastatic laryngocarcinoma cells compared to controls. Bioinformatic 
      analysis identified that 19 key microRNAs might involve in LSCC development. 
      Moreover, RT-PCR confirmed that miR-25, miR-100, miR-125b-5p and let-7g were 
      differentially expressed in different laryngocarcinoma cells and human tumor 
      specimens. CONCLUSIONS AND SIGNIFICANCE: Our findings suggest that microRNA play 
      an important role in the invasion and metastasis of LSCC, and provide the clues 
      for studying the function of microRNA as well as opportunities to analyze the 
      complex molecular abnormalities driving LSCC progression.
FAU - Chen, Liwei
AU  - Chen L
AD  - a Otolaryngology Head and Neck Surgery , Hainan Branch of Chinese PLA General 
      Hospital , Sanya , China.
AD  - b Otolaryngology Head and Neck Surgery , General Hospital of People's Liberation 
      Army , Beijing , China.
FAU - Liu, Shaoyan
AU  - Liu S
AD  - c Peking Union Medical College, Department of Head and Neck Surgery , Cancer 
      Hospital, Chinese Academy of Medical Sciences , Beijing , China.
FAU - Li, Kun
AU  - Li K
AD  - d Otolaryngology , Capital Medical University Affiliated Anzhen Hospital , 
      Beijing , China.
FAU - Qi, Jixia
AU  - Qi J
AD  - e Otolaryngology Head and Neck Surgery , Weifang Medical University , Weifang , 
      China.
FAU - Liu, Chenqing
AU  - Liu C
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
FAU - Zong, Liang
AU  - Zong L
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
FAU - Zhang, Yongxia
AU  - Zhang Y
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
FAU - Zhao, Jiandong
AU  - Zhao J
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
FAU - Zhai, Xingyou
AU  - Zhai X
AD  - g Otolaryngology Head and Neck Surgery , Hainan Branch of Chinese PLA General 
      Hospital , Sanya , China.
FAU - Li, Jianhui
AU  - Li J
AD  - g Otolaryngology Head and Neck Surgery , Hainan Branch of Chinese PLA General 
      Hospital , Sanya , China.
FAU - Kong, Xinru
AU  - Kong X
AD  - e Otolaryngology Head and Neck Surgery , Weifang Medical University , Weifang , 
      China.
FAU - Yang, Shiming
AU  - Yang S
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
FAU - Liu, Mingbo
AU  - Liu M
AD  - f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , 
      China.
AD  - g Otolaryngology Head and Neck Surgery , Hainan Branch of Chinese PLA General 
      Hospital , Sanya , China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20190131
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/mortality/*pathology
MH  - China
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/mortality/*pathology
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness/pathology
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Sampling Studies
MH  - Survival Analysis
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - MicroRNA
OT  - bioinformatic analysis
OT  - invasion and metastasis
OT  - laryngeal squamous cell carcinoma
EDAT- 2019/02/01 06:00
MHDA- 2019/06/14 06:00
CRDT- 2019/02/01 06:00
PHST- 2019/02/01 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2019/02/01 06:00 [entrez]
AID - 10.1080/00016489.2018.1508887 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2018 Dec;138(12):1105-1111. doi: 10.1080/00016489.2018.1508887. 
      Epub 2019 Jan 31.