PMID- 30702178 OWN - NLM STAT- MEDLINE DCOM- 20190506 LR - 20190506 IS - 1099-0801 (Electronic) IS - 0269-3879 (Linking) VI - 33 IP - 5 DP - 2019 May TI - Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat. PG - e4501 LID - 10.1002/bmc.4501 [doi] AB - The present study was carried out to determine 16 antibiotics belonging to seven different groups (tetracyclines, sulfonamides, penicillins, fluoroquinolones, macrolides, lincosamides and trimethoprims) in duck meat. A solid-phase extraction method based on Oasis HLB cartridges coupled with liquid chromatography-electrospray ionization tandem mass spectrometry was developed. Solutions of 0.1 m ethylenediaminetetraacetic acid disodium salt and 2% trifluoroacetic acid were used for the preliminary extraction of the target antibiotics from duck meat and n-hexane was used for purification prior to solid-phase extraction. Mobile phases composed of 0.1% trifluoroacetic acid in distilled water (solvent A) and 0.1% trifluoroacetic acid in methanol (solvent B), combined with a reversed-phase C(18) analytical column, provided the optimal separation and signal intensity. The linearity of the method was assessed using six concentrations (5, 10, 20, 30, 40, and 50 mug/kg), and the recoveries, which were calculated at three spiking concentrations (5, 10 and 20 mug/kg), were in the range 69.8-103.3% with relative standard deviations (RSDs)