PMID- 30703225
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20190826
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 36
IP  - 2
DP  - 2019 Feb 10
TI  - [Molecular cytogenetic characterization of five patients with myeloid leukemia 
      and t(12;22)(p13;q12)].
PG  - 112-115
LID - 10.3760/cma.j.issn.1003-9406.2019.02.004 [doi]
AB  - OBJECTIVE: To explore the clinical and laboratory characteristics of 5 patients 
      with myeloid leukemia and t(12;22)(p13;q12). METHODS: Bone marrow cells were 
      cultured for 24 h and analyzed by standard R-banding. Rearrangement of the MN1 
      gene was detected by fluorescence in situ hybridization (FISH) using dual color 
      break-apart MN1 probes. MN1-ETV6 and ETV6-MN1 fusion genes were detected by 
      reverse transcription polymerase chain reaction (RT-PCR). And the products were 
      subjected to direct sequencing. RESULTS: Among the 5 patients, 2 had AML-M0, 2 
      had AML-M4, and 1 had CMM(0)L at the initial diagnosis. t(12;22)(p13;q12) was the 
      primary abnormality among all patients. Rearrangements of MN1 gene were detected 
      by FISH in all patients. MN1-ETV6 and ETV6-MN1 fusion genes were detected 
      respectively in 4 and 3 patients. CONCLUSION: t(12;22)(p13;q12) is a rare but 
      recurrent chromosomal abnormality in myeloid leukemia, and is related to poor 
      prognosis. allo-SCT is valuable for patients with t(12;22)(p13;q12).
FAU - Shao, Haigang
AU  - Shao H
AD  - The First Affiliated Hospital of Soochow University, Jiangsu Institute of 
      Hematology, Key Laboratory of Thrombosis and Hemostasis of the Ministry of 
      Health, Suzhou, Jiangsu 215006, China. jinlanpan@126.com.
FAU - Yang, Qian
AU  - Yang Q
FAU - Gong, Yanlei
AU  - Gong Y
FAU - Bai, Shuxiao
AU  - Bai S
FAU - Zhang, Jun
AU  - Zhang J
FAU - Wang, Yong
AU  - Wang Y
FAU - Shen, Juan
AU  - Shen J
FAU - Wu, Chunxiao
AU  - Wu C
FAU - Qiu, Huiying
AU  - Qiu H
FAU - Chen, Suning
AU  - Chen S
FAU - Pan, Jinlan
AU  - Pan J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Oncogene Proteins, Fusion)
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 22
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Myeloid/genetics
MH  - Oncogene Proteins, Fusion
MH  - *Translocation, Genetic
EDAT- 2019/02/01 06:00
MHDA- 2019/08/27 06:00
CRDT- 2019/02/01 06:00
PHST- 2019/02/01 06:00 [entrez]
PHST- 2019/02/01 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
AID - 940636003 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2019.02.004 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2019 Feb 10;36(2):112-115. doi: 
      10.3760/cma.j.issn.1003-9406.2019.02.004.