PMID- 30703600
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20191217
IS  - 1878-8769 (Electronic)
IS  - 1878-8750 (Linking)
VI  - 125
DP  - 2019 May
TI  - Relationship Between Apoptosis of Endplate Microvasculature and Degeneration of 
      the Intervertebral Disk.
PG  - e392-e397
LID - S1878-8750(19)30183-4 [pii]
LID - 10.1016/j.wneu.2019.01.085 [doi]
AB  - OBJECTIVE: To explore the relationship between intervertebral disk degeneration 
      and endplate microvasculature, and to determine the role of apoptosis in the 
      pathophysiology underlying end plate microvasculature. METHODS: Twelve 
      6-month-old rabbits were randomly divided into group A (control group where 
      animals underwent a sham operation, in which the loading device was implanted but 
      without loading) and group B (degeneration group, where a calibrated spring 
      within the loading device would immediately create static shear force of 50 N to 
      the disk of L4-5). Paraffin-embedded midsagittal sections of the L4-5 disk were 
      obtained 4 weeks after surgery in the both groups. Sections were stained with 
      cluster of differentiation (CD) 31 immunohistochemistry to measure the blood 
      vessel density in the endplate, with CD31 immunofluorescence and terminal dUTP 
      nick-end labeling (TUNEL) to detect the apoptosis of vascular endothelial cells 
      in the endplate. RESULTS: After 4 weeks, the microvasculature density was 91 +/- 8 
      vessels/mm(2) in group A and 47 +/- 2 vessels/mm(2) (P < 0.001) in group B, 
      demonstrating that vessels were reduced in the endplate of intervertebral disk 
      degeneration. CD31 immunofluorescence and TUNEL showed that apoptosis of vascular 
      endothelial cells exists in the endplate of intervertebral disk degeneration. 
      CONCLUSIONS: The results of this study suggest that apoptosis of vascular 
      endothelial cells results in a decrease in endplate microvasculature density, 
      further affecting the pathologic process of intervertebral disk degeneration.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Xu, Hong-Ming
AU  - Xu HM
AD  - Department of Orthopaedic Surgery, Affiliated Cixi Hospital of Wenzhou Medical 
      University, Cixi, Ningbo, People's Republic of China.
FAU - Hu, Fei
AU  - Hu F
AD  - Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, People's 
      Republic of China.
FAU - Wang, Xiang-Yang
AU  - Wang XY
AD  - Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, People's Republic of China. Electronic address: 
      xiangyangwang@126.com.
FAU - Tong, Song-Lin
AU  - Tong SL
AD  - Department of Orthopaedic Surgery, Affiliated Cixi Hospital of Wenzhou Medical 
      University, Cixi, Ningbo, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190128
PL  - United States
TA  - World Neurosurg
JT  - World neurosurgery
JID - 101528275
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - Intervertebral Disc/blood supply/*pathology
MH  - Intervertebral Disc Degeneration/*pathology
MH  - Lumbar Vertebrae/blood supply/*surgery
MH  - Magnetic Resonance Imaging
MH  - Microvessels/pathology
MH  - Rabbits
MH  - Random Allocation
MH  - Specimen Handling
OTO - NOTNLM
OT  - Apoptosis
OT  - Blood vessel
OT  - Endplate
OT  - Intervertebral disk degeneration
OT  - TUNEL
EDAT- 2019/02/01 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/02/01 06:00
PHST- 2018/09/18 00:00 [received]
PHST- 2019/01/15 00:00 [revised]
PHST- 2019/01/17 00:00 [accepted]
PHST- 2019/02/01 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/02/01 06:00 [entrez]
AID - S1878-8750(19)30183-4 [pii]
AID - 10.1016/j.wneu.2019.01.085 [doi]
PST - ppublish
SO  - World Neurosurg. 2019 May;125:e392-e397. doi: 10.1016/j.wneu.2019.01.085. Epub 
      2019 Jan 28.