PMID- 30704398
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20200225
IS  - 1471-2229 (Electronic)
IS  - 1471-2229 (Linking)
VI  - 19
IP  - 1
DP  - 2019 Jan 31
TI  - Transcriptomic analysis of Citrus clementina mandarin fruits maturation reveals a 
      MADS-box transcription factor that might be involved in the regulation of 
      earliness.
PG  - 47
LID - 10.1186/s12870-019-1651-z [doi]
LID - 47
AB  - BACKGROUND: Harvest time is a relevant economic trait in citrus, and selection of 
      cultivars with different fruit maturity periods has a remarkable impact in the 
      market share. Generation of early- and late-maturing cultivars is an important 
      target for citrus breeders, therefore, generation of knowledge regarding the 
      genetic mechanisms controlling the ripening process and causing the early and 
      late phenotypes is crucial. In this work we analyze the evolution of the 
      transcriptome during fruit ripening in 3 sport mutations derived from the Fina 
      clementine (Citrus clementina) mandarin: Clemenules (CLE), Arrufatina (ARR) and 
      Hernandina (HER) that differ in their harvesting periods. CLE is considered a 
      mid-season cultivar while ARR and HER are early- and late-ripening mutants, 
      respectively. RESULTS: We used RNA-Seq technology to carry out a time course 
      analysis of the transcriptome of the 3 mutations along the ripening period. The 
      results indicated that in these mutants, earliness and lateness during fruit 
      ripening correlated with the advancement or delay in the expression of a set of 
      genes that may be implicated in the maturation process. A detailed analysis of 
      the transcription factors known to be involved in the regulation of fruit 
      ripening identified a member of the MADS box family whose expression was lower in 
      ARR, the early-ripening mutant, and higher in HER, the late-ripening mutant. The 
      pattern of expression of this gene during the maturation period was basically 
      contrary to those of the ethylene biosynthetic genes, SAM and ACC synthases and 
      ACC oxidase. The gene was present in hemizygous dose in the early-ripening 
      mutant. CONCLUSIONS: Our analysis provides new clues about the genetic control of 
      fruit ripening in citrus and allowed the identification of a transcription factor 
      that could be involved in the early phenotype.
FAU - Terol, Javier
AU  - Terol J
AUID- ORCID: 0000-0003-3345-0078
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      46113, Moncada, Valencia, Spain. terol_javalc@gva.es.
FAU - Nueda, M Jose
AU  - Nueda MJ
AD  - Facultad de Ciencias, Universidad de Alicante, Alicante, Spain.
FAU - Ventimilla, Daniel
AU  - Ventimilla D
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      46113, Moncada, Valencia, Spain.
FAU - Tadeo, Francisco
AU  - Tadeo F
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      46113, Moncada, Valencia, Spain.
FAU - Talon, Manuel
AU  - Talon M
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      46113, Moncada, Valencia, Spain.
LA  - eng
GR  - RTA-00071-C06-01/Ministerio de Economia, Industria y Competitividad, Gobierno de 
      Espana/
PT  - Journal Article
DEP - 20190131
PL  - England
TA  - BMC Plant Biol
JT  - BMC plant biology
JID - 100967807
RN  - 0 (MADS Domain Proteins)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Citrus/genetics/*growth & development/metabolism
MH  - Fruit/*growth & development/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Plant
MH  - Genes, Plant/genetics/physiology
MH  - MADS Domain Proteins/genetics/*physiology
MH  - Plant Proteins/genetics/*physiology
MH  - Quantitative Trait, Heritable
PMC - PMC6357379
OTO - NOTNLM
OT  - Citrus
OT  - Earliness
OT  - Fruit
OT  - Lateness
OT  - MADS box
OT  - RNA-Seq
OT  - Ripening
OT  - Transcription factor
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable CONSENT FOR 
      PUBLICATION: Not applicable COMPETING INTERESTS: The authors declare that they 
      have no competing interests PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2019/02/02 06:00
MHDA- 2019/03/08 06:00
PMCR- 2019/01/31
CRDT- 2019/02/02 06:00
PHST- 2018/09/12 00:00 [received]
PHST- 2019/01/14 00:00 [accepted]
PHST- 2019/02/02 06:00 [entrez]
PHST- 2019/02/02 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2019/01/31 00:00 [pmc-release]
AID - 10.1186/s12870-019-1651-z [pii]
AID - 1651 [pii]
AID - 10.1186/s12870-019-1651-z [doi]
PST - epublish
SO  - BMC Plant Biol. 2019 Jan 31;19(1):47. doi: 10.1186/s12870-019-1651-z.