PMID- 30706572
OWN - NLM
STAT- MEDLINE
DCOM- 20201117
LR  - 20201117
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 41
IP  - 7
DP  - 2019 Jul
TI  - Aurora kinase A induces chemotherapy resistance through revival of dormant cells 
      in laryngeal squamous cell carcinoma.
PG  - 2239-2248
LID - 10.1002/hed.25689 [doi]
AB  - BACKGROUND: Chemotherapy resistance was an important tumor metastasis mechanism. 
      METHODS: Cell Counting Kit-8 assay and plate colony formation assay were applied 
      to examine the proliferation of laryngeal squamous cell carcinoma (LSCC). 
      Immunofluorescent staining and Western blotting were carried out to show the 
      expression of related proteins. Wound healing, migration, and invasion assays 
      were used to examine the mobility, migration, and invasion of LSCC. RESULTS: 
      Downregulated Aurora kinase A (AURKA) increased chemotherapy sensitivity and 
      reduced the ability of mobility, migration, and invasion of Hep2 cells, while 
      upregulated AURKA possessed opposite results. Hep2/5-Fu cells possessed 
      dormancy-like properties and upregulated AURKA in Hep2/5-Fu cells 
      (Hep2/5-Fu/AURKA cells) revived dormant state. Furthermore, Erk1/2 was restrained 
      in Hep2/5-Fu cells and activated in Hep2/5-Fu/AURKA cells. Moreover, Erk1/2 
      accelerated the ability of mobility, migration, and invasion in Hep2/5-Fu/AURKA 
      cells. CONCLUSION: AURKA activated dormant state to induce chemotherapy 
      resistance and promoted metastasis of LSCC through Erk1/2 pathway.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Yang, Li-Yun
AU  - Yang LY
AD  - Department of Otolaryngology, Gongli Hospital, The Second Military Medical 
      University, Shanghai, China.
FAU - Shan, Ya-Min
AU  - Shan YM
AD  - Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Otolaryngology, Gongli Hospital, The Second Military Medical 
      University, Shanghai, China.
FAU - Zhou, En-Hui
AU  - Zhou EH
AD  - Department of Otolaryngology, Gongli Hospital, The Second Military Medical 
      University, Shanghai, China.
FAU - Chen, Xiao-Ping
AU  - Chen XP
AD  - Department of Otolaryngology, Gongli Hospital, The Second Military Medical 
      University, Shanghai, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai 
      Jiaotong University, Shanghai, China.
LA  - eng
GR  - SHDC12015144/Clinical Science and Technology Innovation Project of Shengkang 
      Hospital Development Center/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190201
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - EC 2.7.11.1 (Aurora Kinase A)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
EIN - Head Neck. 2019 Aug;41(8):2839. PMID: 31294903
MH  - Antimetabolites, Antineoplastic/pharmacology
MH  - Aurora Kinase A/*metabolism
MH  - Carcinoma, Squamous Cell/drug therapy/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Down-Regulation
MH  - *Drug Resistance, Neoplasm
MH  - Fluorouracil/pharmacology
MH  - Humans
MH  - Laryngeal Neoplasms/drug therapy/*pathology
MH  - MAP Kinase Signaling System/physiology
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Phosphorylation
MH  - Up-Regulation
OTO - NOTNLM
OT  - 5-Fu resistance
OT  - Erk1/2
OT  - LSCC
OT  - aurora kinase A
OT  - dormancy
EDAT- 2019/02/02 06:00
MHDA- 2020/11/18 06:00
CRDT- 2019/02/02 06:00
PHST- 2018/03/20 00:00 [received]
PHST- 2018/12/12 00:00 [revised]
PHST- 2019/01/15 00:00 [accepted]
PHST- 2019/02/02 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2019/02/02 06:00 [entrez]
AID - 10.1002/hed.25689 [doi]
PST - ppublish
SO  - Head Neck. 2019 Jul;41(7):2239-2248. doi: 10.1002/hed.25689. Epub 2019 Feb 1.