PMID- 30708098
OWN - NLM
STAT- MEDLINE
DCOM- 20190215
LR  - 20190215
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 220
DP  - 2019 Mar 1
TI  - Binge ethanol and MDMA combination exacerbates HSP27 and Trx-1 (biomarkers of 
      toxic cardiac effects) expression in right ventricle.
PG  - 50-57
LID - S0024-3205(19)30063-3 [pii]
LID - 10.1016/j.lfs.2019.01.050 [doi]
AB  - AIMS: Oxidative stress caused by exposure to drugs of abuse such as ethanol or 3, 
      4 methylenedioxymethamphetamine (MDMA) may derive from direct or indirect effects 
      in many organs including the heart. The aim of the present work was to evaluate 
      cardiac sympathetic activity and the expression and activation of two antioxidant 
      proteins: heat shock protein27 (HSP27) and thioredoxin-1 (Trx-1) after voluntary 
      binge ethanol consumption, alone and in combination with MDMA. MATERIAL AND 
      METHODS: Adolescent mice received MDMA, ethanol or both. Drinking in the dark 
      (DID) procedure was used as a model of binge. HSP27 expression and 
      phosphorylation at serine 82 (pHSP27), Trx-1 expression, tyrosine hydroxylase 
      (TH) and TH phosphorylated at serine 31 (pTH) were evaluated in adolescent mice 
      48 h and 7 days after treatments in the right ventricle. TH, HSP27 expression and 
      phosphorylation and Trx-1 expression were measured by quantitative blot 
      immunolabeling using specific antibodies. KEY FINDINGS: The expression of HSP27, 
      pHSP27, Trx-1, total TH and pTH in the right ventricle was increased after binge 
      ethanol or MDMA alone. In addition, the combination of binge ethanol + MDMA 
      enhanced TH expression and phosphorylation versus their individual 
      administration. SIGNIFICANCE: These results indicate that this combination could 
      produce higher activation of sympathetic pathways, which could trigger an 
      increased cell stress. On the other hand, increased HSP27, pHSP27 and Trx-1 
      expression in the right ventricle by ethanol + MDMA could be a protective 
      mechanism to reduce the adverse effects of oxidative stress caused by both drugs 
      of abuse.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Navarro-Zaragoza, Javier
AU  - Navarro-Zaragoza J
AD  - Department of Pharmacology, Faculty of Medicine, University of Murcia, Spain; 
      Instituto Murciano de Investigacion Biosanitaria, Murcia, Spain. Electronic 
      address: jnavarrozaragoza@um.es.
FAU - Ros-Simo, Clara
AU  - Ros-Simo C
AD  - Grup de Recerca en Neurobiologia del Comportament (GRNC), Universitat Pompeu 
      Fabra, Barcelona, Spain.
FAU - Milanes, Maria-Victoria
AU  - Milanes MV
AD  - Department of Pharmacology, Faculty of Medicine, University of Murcia, Spain; 
      Instituto Murciano de Investigacion Biosanitaria, Murcia, Spain.
FAU - Valverde, Olga
AU  - Valverde O
AD  - Grup de Recerca en Neurobiologia del Comportament (GRNC), Universitat Pompeu 
      Fabra, Barcelona, Spain.
FAU - Laorden, Maria-Luisa
AU  - Laorden ML
AD  - Department of Pharmacology, Faculty of Medicine, University of Murcia, Spain; 
      Instituto Murciano de Investigacion Biosanitaria, Murcia, Spain.
LA  - eng
PT  - Journal Article
DEP - 20190129
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Biomarkers)
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (Hspb2 protein, mouse)
RN  - 0 (Txn1 protein, mouse)
RN  - 3K9958V90M (Ethanol)
RN  - 52500-60-4 (Thioredoxins)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Binge Drinking/metabolism
MH  - Biomarkers/metabolism
MH  - Body Temperature/drug effects
MH  - Cardiotoxicity/metabolism
MH  - Ethanol/adverse effects/metabolism
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - HSP27 Heat-Shock Proteins/*drug effects/metabolism
MH  - Heart/drug effects
MH  - Heart Ventricles/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - N-Methyl-3,4-methylenedioxyamphetamine/adverse effects/metabolism
MH  - Oxidative Stress
MH  - Phosphorylation
MH  - Thioredoxins/*drug effects/metabolism
OTO - NOTNLM
OT  - Binge ethanol
OT  - Heat shock protein 27
OT  - MDMA
OT  - Right ventricle
OT  - Thioredoxin-1
EDAT- 2019/02/02 06:00
MHDA- 2019/02/16 06:00
CRDT- 2019/02/02 06:00
PHST- 2018/10/17 00:00 [received]
PHST- 2019/01/10 00:00 [revised]
PHST- 2019/01/28 00:00 [accepted]
PHST- 2019/02/02 06:00 [pubmed]
PHST- 2019/02/16 06:00 [medline]
PHST- 2019/02/02 06:00 [entrez]
AID - S0024-3205(19)30063-3 [pii]
AID - 10.1016/j.lfs.2019.01.050 [doi]
PST - ppublish
SO  - Life Sci. 2019 Mar 1;220:50-57. doi: 10.1016/j.lfs.2019.01.050. Epub 2019 Jan 29.