PMID- 30708975
OWN - NLM
STAT- MEDLINE
DCOM- 20190618
LR  - 20220410
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 3
DP  - 2019 Jan 31
TI  - Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal 
      Stem Cells to Treat Osseous Defects.
LID - 10.3390/ijms20030612 [doi]
LID - 612
AB  - Lack of standardization of clinically compliant culture protocols of mesenchymal 
      stem cells for re-implantation in humans have hindered clinical progress in the 
      field of tissue regeneration to repair maxillofacial and orthopedic defects. The 
      goal of this study was to establish a clinically relevant osteogenic protocol for 
      collection and expansion of autologous stem cells to be used at Marshall 
      University for re-implantation and repair of maxillofacial and orthopedic 
      conditions. Human bone marrow (hBM) samples were collected from patients 
      undergoing intramedullary nail fixation for closed femoral fractures. hBM 
      mesenchymal cells were expanded by growing them first in Petri dishes for two 
      weeks, followed by a week of culture using Perfecta 3D Hanging Drop Plates((R)). 
      Various scaffold materials were tested and analyzed for cellular integration, 
      vitality, and differentiation capacity of harvested hBM-MSCs including: 60/40 
      blend of hydroxyapatite biomatrix; Acellular bone composite discs; Allowash((R)), 
      cancellous bone cubes; PLGA (poly lactic-co-glycolic acid); and Woven chitin 
      derived fiber. We found that the 3D spheroid culture allowed production of hBM 
      mesenchymal cells that retained osteoblast differentiation capacity over a 
      monolayer culture of hBM-MSCs without the need to use chemical or hormonal 
      modulation. We also observed that hydroxyapatite and Allowash cancellous bone 
      scaffolds allowed better cell integration and viability properties as compared to 
      other materials tested in this study. In conclusion, the multimodal culture 
      methodology we developed creates actively differentiating stem-cell spheroids 
      that can then be readily utilized in clinical practices to improve the 
      regeneration of tissues of the head and the body.
FAU - Lawrence, Logan M
AU  - Lawrence LM
AD  - Cabell Huntington Hospital Laboratory, Department of Pathology, Marshall 
      University Joan C. Edwards School of Medicine, Huntington, WV 25705, USA. 
      loganlawrence38@gmail.com.
FAU - Cottrill, Andrew
AU  - Cottrill A
AD  - Department of Biological Sciences, Marshall University, Huntington, WV 25705, 
      USA. cottrill40@live.marshall.edu.
FAU - Valluri, Amrita
AU  - Valluri A
AD  - Department of Biological Sciences, Marshall University, Huntington, WV 25705, 
      USA. valluri3@live.marshall.edu.
FAU - Marenzi, Gaetano
AU  - Marenzi G
AD  - Department of Neuroscience, Reproductive, and Odontostomatologic Sciences, 
      University of Naples "Federico II" 80131, Italy. gaetano.marenzi@gmail.com.
FAU - Denning, Krista L
AU  - Denning KL
AD  - Cabell Huntington Hospital Laboratory, Department of Pathology, Marshall 
      University Joan C. Edwards School of Medicine, Huntington, WV 25705, USA. 
      haught5@marshall.edu.
FAU - Valluri, Jagan
AU  - Valluri J
AD  - Department of Biological Sciences, Marshall University, Huntington, WV 25705, 
      USA. valluri@marshall.edu.
FAU - Claudio, Pier Paolo
AU  - Claudio PP
AUID- ORCID: 0000-0001-7790-1622
AD  - Department of BioMolecular Sciences, National Center for Natural Product 
      Research, University of Mississippi, University, MS 39216, USA. 
      pclaudio@olemiss.edu.
FAU - Day, James B
AU  - Day JB
AD  - Department of Orthopaedic Surgery, Marshall University Joan C. Edwards School of 
      Medicine, Huntington, WV 25705, USA. day62@marshall.edu.
LA  - eng
PT  - Journal Article
DEP - 20190131
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Adult
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Child
MH  - Durapatite/chemistry
MH  - Female
MH  - Fractures, Bone/*therapy
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Osteogenesis
MH  - Regenerative Medicine
MH  - Spheroids, Cellular/*cytology
MH  - Tissue Scaffolds/*chemistry
MH  - Transplantation, Autologous
PMC - PMC6387189
OTO - NOTNLM
OT  - autologous transplant
OT  - bone defects
OT  - bone marrow mesenchymal stem cells
OT  - fracture healing
OT  - regenerative medicine
OT  - traumatic injuries
COIS- All authors have read the journal's policy on disclosure of potential conflict of 
      interest and all the authors declare that they have no any financial or personal 
      relationship with organizations that could potentially be perceived as 
      influencing the described research.
EDAT- 2019/02/03 06:00
MHDA- 2019/06/19 06:00
PMCR- 2019/02/01
CRDT- 2019/02/03 06:00
PHST- 2019/01/08 00:00 [received]
PHST- 2019/01/26 00:00 [revised]
PHST- 2019/01/27 00:00 [accepted]
PHST- 2019/02/03 06:00 [entrez]
PHST- 2019/02/03 06:00 [pubmed]
PHST- 2019/06/19 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - ijms20030612 [pii]
AID - ijms-20-00612 [pii]
AID - 10.3390/ijms20030612 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Jan 31;20(3):612. doi: 10.3390/ijms20030612.