PMID- 30710349
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 9
DP  - 2019 Sep
TI  - NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary 
      epithelial cells via the mTOR signaling pathway.
PG  - 15825-15835
LID - 10.1002/jcp.28240 [doi]
AB  - Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a 
      highly phosphorylated nuclear protein ubiquitously expressed in vertebrates. 
      NUCKS1 has been reported to be a key chromatin modifier and transcriptional 
      regulator of a number of signaling pathways, but the physiological role and 
      detailed mechanism are still limited. In this study, we assessed the role of 
      NUCKS1 on milk synthesis in and proliferation of mammary epithelial cells from a 
      dairy cow. NUCKS1 was located in the nucleus of mammary epithelial cells, and the 
      expression of NUCKS1 was stimulated by amino acids (Met and Leu) and hormones 
      (estrogen and prolactin). Gene function study approaches detected that NUCKS1 
      positively regulated milk protein, milk fat, and lactose synthesis, and also 
      increased the cell number, cell viability, and cell cycle progression. NUCKS1 
      mediated the stimulation of amino acids and hormones on the messenger RNA 
      expression of the mechanistic target of rapamycin (mTOR), SREBP-1c, and Cyclin 
      D1. The expression of NUCKS1 is dramatically higher in mouse mammary tissue of 
      lactating period, compared with that in puberty and dry period. Taken together, 
      these results reveal that NUCKS1 is a new mediator of milk synthesis in and 
      proliferation of mammary epithelial cells via regulating the mTOR signaling 
      pathway.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Yuan, Xiaohan
AU  - Yuan X
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Zhang, Minghui
AU  - Zhang M
AUID- ORCID: 0000-0002-8253-1379
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Ao, Jinxia
AU  - Ao J
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Zhen, Zhen
AU  - Zhen Z
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Gao, Xuejun
AU  - Gao X
AUID- ORCID: 0000-0002-8241-6346
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Li, Meng
AU  - Li M
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
LA  - eng
GR  - 31472162/National Natural Science Foundation of China/
GR  - 31671473/National Natural Science Foundation of China/
GR  - C2017021/Natural Science Foundation of Heilongjiang Province of China/
PT  - Journal Article
DEP - 20190202
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
SB  - IM
OTO - NOTNLM
OT  - Cyclin D1
OT  - SREBP-1c
OT  - mammary epithelial cells
OT  - mechanistic target of rapamycin (mTOR)
OT  - nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1)
EDAT- 2019/02/03 06:00
MHDA- 2019/02/03 06:01
CRDT- 2019/02/03 06:00
PHST- 2019/01/06 00:00 [revised]
PHST- 2018/11/23 00:00 [received]
PHST- 2019/01/10 00:00 [accepted]
PHST- 2019/02/03 06:00 [pubmed]
PHST- 2019/02/03 06:01 [medline]
PHST- 2019/02/03 06:00 [entrez]
AID - 10.1002/jcp.28240 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Sep;234(9):15825-15835. doi: 10.1002/jcp.28240. Epub 2019 
      Feb 2.