PMID- 30712664
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 75
DP  - 2019 Jan
TI  - Analysis of the effects of polyunsaturated fatty acids on transporter expressions 
      using a PCR array: Induction of xCT/SLC7A11 in human placental BeWo cells.
PG  - 34-41
LID - S0143-4004(18)30665-9 [pii]
LID - 10.1016/j.placenta.2018.11.010 [doi]
AB  - OBJECTIVE: Polyunsaturated fatty acids (PUFAs), including arachidonic acid (AA), 
      eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are essential for 
      adequate fetal growth. The aim of the present study was to elucidate the effects 
      of PUFAs on the expression and function of placental transporters, which play 
      important roles in placental functions including the supply of nutrients to the 
      fetus, excretion of metabolites, and protection of the fetus from xenobiotics. 
      METHODS: Human placental choriocarcinoma BeWo cells were used as a trophoblast 
      model. PUFA-induced alteration in the gene expression of 84 transporters was 
      investigated by a commercially available PCR array. Protein levels and the 
      activity of transporters were assessed by western blotting and uptake 
      experiments, respectively. The placental expression of the transporters was 
      analyzed using pregnant Wistar rats. RESULTS: PUFAs (AA, EPA, and DHA) increased 
      cystine/glutamate transporter xCT/SLC7A11, which mediates the cellular uptake of 
      cystine coupled with the efflux of glutamate in human placental choriocarcinoma 
      BeWo cells. These PUFAs also increased [(14)C]-cystine uptake in BeWo cells. 
      PUFA-induced xCT/SLC7A11 mRNA expression was not blocked by nuclear 
      factor-erythroid 2-related factor-2 (NRF2) knockdown. Reverse transcription 
      (RT)-PCR analysis indicated that xCT/Slc7a11 mRNA was detected in rat placenta 
      and the expression level at gestational day (GD) 12 was higher than that at GD 
      20. CONCLUSION: These results indicate that PUFAs promoted cystine uptake in 
      placental cells by inducing xCT/SLC7A11 expression and NRF2 did not contribute to 
      upregulation of xCT/SLC7A11 by PUFAs. Furthermore, xCT expression in rat placenta 
      may change during pregnancy.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Ono, Kanako
AU  - Ono K
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan.
FAU - Furugen, Ayako
AU  - Furugen A
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan.
FAU - Kurosawa, Yuko
AU  - Kurosawa Y
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan.
FAU - Jinno, Naoko
AU  - Jinno N
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan.
FAU - Narumi, Katsuya
AU  - Narumi K
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan.
FAU - Kobayashi, Masaki
AU  - Kobayashi M
AD  - Department of Pharmacy, Hokkaido University Hospital, Kita-14-jo, Nishi-5-chome, 
      Kita-ku, Sapporo, 060-8648, Japan.
FAU - Iseki, Ken
AU  - Iseki K
AD  - Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, 
      Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, 
      Nishi-6-chome, Kita-ku, Sapporo, 060-0812, Japan; Department of Pharmacy, 
      Hokkaido University Hospital, Kita-14-jo, Nishi-5-chome, Kita-ku, Sapporo, 
      060-8648, Japan. Electronic address: ken-i@pharm.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181127
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Amino Acid Transport System y+)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (SLC7A11 protein, human)
SB  - IM
MH  - Amino Acid Transport System y+/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Fatty Acids, Unsaturated/*physiology
MH  - Female
MH  - Humans
MH  - NF-E2-Related Factor 2/metabolism
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Arachidonic acid (AA)
OT  - Docosahexaenoic acid (DHA)
OT  - Eicosapentaenoic acid (EPA)
OT  - Placenta
OT  - Transporter
OT  - xCT/SLC7A11
EDAT- 2019/02/05 06:00
MHDA- 2020/03/17 06:00
CRDT- 2019/02/05 06:00
PHST- 2018/07/18 00:00 [received]
PHST- 2018/11/04 00:00 [revised]
PHST- 2018/11/26 00:00 [accepted]
PHST- 2019/02/05 06:00 [entrez]
PHST- 2019/02/05 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
AID - S0143-4004(18)30665-9 [pii]
AID - 10.1016/j.placenta.2018.11.010 [doi]
PST - ppublish
SO  - Placenta. 2019 Jan;75:34-41. doi: 10.1016/j.placenta.2018.11.010. Epub 2018 Nov 
      27.