PMID- 30712927
OWN - NLM
STAT- MEDLINE
DCOM- 20190617
LR  - 20211204
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 102
IP  - 4
DP  - 2019 Apr
TI  - Parenteral administration of l-arginine to twin-bearing Romney ewes during late 
      pregnancy is associated with reduced milk somatic cell count during early 
      lactation.
PG  - 3071-3081
LID - S0022-0302(19)30096-7 [pii]
LID - 10.3168/jds.2018-15433 [doi]
AB  - Maternal milk is the primary source of nutrition for suckling mammals, and its 
      yield and composition are important determinants of survival during the early 
      neonatal period. The objective of this study was to examine whether parenteral 
      administration of l-Arg to twin-bearing ewes, during mid to late pregnancy, 
      influenced prepartum maternal mammary gland development and subsequent lactation 
      performance in the early postpartum period (14 d). At 80 d of pregnancy, 
      multiparous Romney ewes were housed indoors in group pens, split into 2 cohorts, 
      and fed a lucerne-based pellet diet, formulated to meet 100% of National Research 
      Council-recommended requirements for twin-bearing pregnant ewes, once a day. 
      Cohort 1 was administered l-Arg (72.7 mg/kg of live weight via i.v, 3 times a 
      day) from d 100 of pregnancy until d 140. At d 140, ewes were euthanized and 
      maternal mammary tissues were collected for analysis of the biochemical indices 
      total DNA, RNA, protein, protein synthetic efficiency (protein:RNA), cell size 
      (protein:DNA), transcriptional efficiency (RNA:DNA), and the abundance of 
      mammalian target of rapamycin (mTOR) and mTOR(Ser2448) protein. Cohort 2 was 
      administered an identical l-Arg regimen as cohort 1, but from d 100 until 
      parturition. Milk was collected over a 14-d period (d 1, 4, 7, 10, and 14) to 
      assess milk yield and composition. In cohort 1, total mammary DNA (cell number) 
      tended to be higher in l-Arg ewes, with no change in total mammary RNA or protein 
      content, biochemical indices of protein synthetic efficiency, cell size or 
      transcriptional efficiency, or mTOR protein abundance or phosphorylation. In 
      cohort 2, milk composition analysis from l-Arg ewes showed lower (d 7-14) milk 
      somatic cell counts, greater crude protein percentage from d 7 to 10 but lower at 
      d 14, and altered absolute concentrations of some free AA (d 7 and 14) compared 
      with controls. We propose that parenteral administration of l-Arg during late 
      pregnancy is associated with increased mammary gland cellular content and 
      decreased somatic cell counts during early lactation.
CI  - Copyright (c) 2019 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Sciascia, Quentin L
AU  - Sciascia QL
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand; Gravida, National 
      Centre for Growth and Development, University of Auckland, Auckland 1023, New 
      Zealand.
FAU - van der Linden, Danitsja S
AU  - van der Linden DS
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand; Gravida, National 
      Centre for Growth and Development, University of Auckland, Auckland 1023, New 
      Zealand.
FAU - Sales, Francisco A
AU  - Sales FA
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand; Gravida, National 
      Centre for Growth and Development, University of Auckland, Auckland 1023, New 
      Zealand.
FAU - Wards, Nina J
AU  - Wards NJ
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand.
FAU - Blair, Hugh T
AU  - Blair HT
AD  - Gravida, National Centre for Growth and Development, University of Auckland, 
      Auckland 1023, New Zealand; International Sheep Research Centre, Massey 
      University, Palmerston North 4442, New Zealand.
FAU - Pacheco, David
AU  - Pacheco D
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand.
FAU - Oliver, Mark H
AU  - Oliver MH
AD  - Gravida, National Centre for Growth and Development, University of Auckland, 
      Auckland 1023, New Zealand; Ngapouri Research Farm, Liggins Institute, University 
      of Auckland, Auckland 3083, New Zealand.
FAU - McCoard, Susan A
AU  - McCoard SA
AD  - AgResearch Grasslands, Palmerston North 4442, New Zealand; Gravida, National 
      Centre for Growth and Development, University of Auckland, Auckland 1023, New 
      Zealand. Electronic address: sue.mccoard@agresearch.co.nz.
LA  - eng
PT  - Journal Article
DEP - 20190201
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 94ZLA3W45F (Arginine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Arginine/*metabolism
MH  - Cell Count
MH  - Cohort Studies
MH  - Dietary Supplements/analysis
MH  - Female
MH  - Humans
MH  - Lactation
MH  - Mammary Glands, Human/metabolism
MH  - Milk/chemistry/*metabolism
MH  - Pregnancy
MH  - Prenatal Nutritional Physiological Phenomena
MH  - Sheep/genetics/*metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Twins
OTO - NOTNLM
OT  - dairy
OT  - l-arginine
OT  - milk composition
OT  - sheep
OT  - somatic cell
EDAT- 2019/02/05 06:00
MHDA- 2019/06/18 06:00
CRDT- 2019/02/05 06:00
PHST- 2018/07/24 00:00 [received]
PHST- 2018/11/30 00:00 [accepted]
PHST- 2019/02/05 06:00 [pubmed]
PHST- 2019/06/18 06:00 [medline]
PHST- 2019/02/05 06:00 [entrez]
AID - S0022-0302(19)30096-7 [pii]
AID - 10.3168/jds.2018-15433 [doi]
PST - ppublish
SO  - J Dairy Sci. 2019 Apr;102(4):3071-3081. doi: 10.3168/jds.2018-15433. Epub 2019 
      Feb 1.