PMID- 30714134
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 9
DP  - 2019 Sep
TI  - MMP2/3 promote the growth and migration of laryngeal squamous cell carcinoma via 
      PI3K/Akt-NF-kappaB-mediated epithelial-mesenchymal transformation.
PG  - 15847-15855
LID - 10.1002/jcp.28242 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is an aggressive malignancy with 
      metastatic potential and high mortality worldwide. Matrix metalloproteinases 
      (MMPs) are a group of extracellular proteolytic enzymes. Although MMPs have been 
      proved to be essential in the process of tumor migration and metastasis in most 
      types of carcinomas, the expression and function of MMP2/3 in LSCC remain 
      unknown. Here, we investigated the roles of MMP2/3 in LSCC and elucidated the 
      underlying mechanism. We found that levels of MMP2/3 were significantly higher in 
      clinical LSCC samples than in paired normal sample examined by real-time 
      polymerase chain reaction and western blot assays. Moreover, overexpression of 
      MMP2/3 promoted the proliferation and migration of LSCC cells by Cell Counting 
      Kit-8, transwell, and scratch wound-healing assays in vitro. Furthermore, levels 
      of epithelial cell markers (E-cadherin and occludin) were decreased following 
      MMP2/3 overexpression, with increased levels of mesenchymal cell markers 
      (N-cadherin and vimentin), suggesting the involvement of epithelial-mesenchymal 
      transformation (EMT) process in MMP2/3-mediated LSCC cell migration. By using 
      short hairpin RNA, knockdown of MMP2/3 expression inhibited the proliferation, 
      migration, and EMT process in LSCC cells in vitro. More importantly, we confirmed 
      that MMP2/3 promoted LSCC in the PI3K/Akt-NF-kappaB-dependent manner. This study 
      provides insight into MMP2/3-mediated LSCC development and lays a foundation for 
      potential pharmacological targets to LSCC.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Zhu, Yi
AU  - Zhu Y
AUID- ORCID: 0000-0002-2222-0106
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
FAU - Yan, Li
AU  - Yan L
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
FAU - Zhu, Wenjia
AU  - Zhu W
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
FAU - Song, Xinmao
AU  - Song X
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
FAU - Yang, Gang
AU  - Yang G
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
FAU - Wang, Shengzi
AU  - Wang S
AD  - Department of Radiation Oncology, Eye and ENT Hospital, Fudan University, 
      Shanghai, China.
LA  - eng
GR  - SHDC12012317/Laryngeal Cancer Prevention and Control Project of Shanghai 
      Shenkang/
PT  - Journal Article
DEP - 20190204
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
SB  - IM
OTO - NOTNLM
OT  - PI3K/Akt-NF-kappaB pathway
OT  - epithelial-mesenchymal transformation
OT  - laryngeal squamous cell carcinoma
OT  - matrix metalloproteinases
OT  - migration
EDAT- 2019/02/05 06:00
MHDA- 2019/02/05 06:01
CRDT- 2019/02/05 06:00
PHST- 2019/01/09 00:00 [revised]
PHST- 2018/11/04 00:00 [received]
PHST- 2019/01/18 00:00 [accepted]
PHST- 2019/02/05 06:00 [pubmed]
PHST- 2019/02/05 06:01 [medline]
PHST- 2019/02/05 06:00 [entrez]
AID - 10.1002/jcp.28242 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Sep;234(9):15847-15855. doi: 10.1002/jcp.28242. Epub 2019 
      Feb 4.