PMID- 30716676
OWN - NLM
STAT- MEDLINE
DCOM- 20190328
LR  - 20190328
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 67
DP  - 2019 Apr
TI  - Effects of perinatal di (2-ethylhexyl) phthalate exposure on thyroid function in 
      rat offspring.
PG  - 53-60
LID - S1382-6689(18)30433-2 [pii]
LID - 10.1016/j.etap.2019.01.012 [doi]
AB  - Di (2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer in industry and 
      displays the characteristics of an endocrine disruptor. Disorders of the maternal 
      thyroid hormone (TH) during pregnancy can cause adverse effects on the fetus. We 
      investigated the effects and possible mechanism of perinatal DEHP exposure on the 
      thyroid function of pups. Pregnant female Wistar rats were randomly divided into 
      four groups and received doses of DEHP of 0, 30, 300, 750 mg/kg/day by gavage at 
      from gestational day (GD) 0 to postnatal day (PN) 21. The concentration of serum 
      THs and the ultrastructure of thyroid follicular cells in the offspring were 
      examined. Related protein level and gene expression of thyroid proteins in pups 
      were analyzed by western blotting and real-time PCR. We found that DEHP 
      significantly reduced total thyroxine (TT(4)) and increased thyroid stimulating 
      hormone (TSH) in pups, while total triiodothyronine (TT(3)) showed no change. 
      Thyroid follicular cells ultrastructure was damaged in DEHP exposed pups as 
      viewed by electron microscopy. Furthermore, exposure to DEHP significantly 
      increased protein and mRNA levels of thyroid transcription factor 1 (TTF-1), 
      paired box 8 (PAX8), sodium iodide symporter (NIS) and thyroid peroxidase (TPO) 
      in pups. In addition, levels of deiodinases of pups were also affected. These 
      findings indicated that DEHP can disrupt thyroid function by damaging thyroid 
      follicles and affecting TTF-1, PAX8, NIS, TPO and the deiodinase protein family.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Dong, Jing
AU  - Dong J
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Cong, Zhangzhao
AU  - Cong Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - You, Mingdan
AU  - You M
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Fu, Yuanyuan
AU  - Fu Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Wang, Yuan
AU  - Wang Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Fu, Hui
AU  - Fu H
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Wei, Lingling
AU  - Wei L
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China.
FAU - Chen, Jie
AU  - Chen J
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, No. 77 Puhe Road, Shenyang 110122, People's Republic of 
      China. Electronic address: jchen@cmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190125
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (PAX8 Transcription Factor)
RN  - 0 (Pax8 protein, rat)
RN  - 0 (Plasticizers)
RN  - 0 (Symporters)
RN  - 0 (TTF1 protein, rat)
RN  - 0 (Transcription Factors)
RN  - 4XE5NDT4K1 (sodium-iodide symporter)
RN  - 9002-71-5 (Thyrotropin)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - EC 1.11.1.8 (Iodide Peroxidase)
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Diethylhexyl Phthalate/*toxicity
MH  - Endocrine Disruptors/*toxicity
MH  - Female
MH  - Iodide Peroxidase/genetics/*metabolism
MH  - Lactation
MH  - Maternal-Fetal Exchange
MH  - PAX8 Transcription Factor/genetics/metabolism
MH  - Plasticizers/*toxicity
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats, Wistar
MH  - Symporters/genetics/metabolism
MH  - Thyroid Gland/*drug effects/ultrastructure
MH  - Thyrotropin/blood
MH  - Thyroxine/blood
MH  - Transcription Factors/genetics/metabolism
OTO - NOTNLM
OT  - DEHP
OT  - Endocrine disruptors
OT  - Offspring
OT  - Perinatal period
OT  - Thyroid function
EDAT- 2019/02/05 06:00
MHDA- 2019/03/29 06:00
CRDT- 2019/02/05 06:00
PHST- 2018/09/26 00:00 [received]
PHST- 2018/12/14 00:00 [revised]
PHST- 2019/01/23 00:00 [accepted]
PHST- 2019/02/05 06:00 [pubmed]
PHST- 2019/03/29 06:00 [medline]
PHST- 2019/02/05 06:00 [entrez]
AID - S1382-6689(18)30433-2 [pii]
AID - 10.1016/j.etap.2019.01.012 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2019 Apr;67:53-60. doi: 10.1016/j.etap.2019.01.012. 
      Epub 2019 Jan 25.