PMID- 30718529
OWN - NLM
STAT- MEDLINE
DCOM- 20200820
LR  - 20231213
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Feb 4
TI  - Effects of a hypomagnetic field on DNA methylation during the differentiation of 
      embryonic stem cells.
PG  - 1333
LID - 10.1038/s41598-018-37372-2 [doi]
LID - 1333
AB  - It has been reported that hypomagnetic fields (HMFs) have a negative influence on 
      mammalian physiological functions. We previously reported that HMFs were 
      detrimental to cell fate changes during reprogramming into pluripotency. These 
      studies led us to investigate whether HMFs affect cell fate determination during 
      direct differentiation. Here, we found that an HMF environment attenuates 
      differentiation capacity and is detrimental to cell fate changes during the in 
      vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions 
      cause abnormal DNA methylation through the dysregulation of DNA 
      methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA 
      methylation during differentiation. Taken together, these results suggest that an 
      appropriate electromagnetic field (EMF) environment may be essential for 
      favorable epigenetic remodeling during cell fate determination via 
      differentiation.
FAU - Baek, Soonbong
AU  - Baek S
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea.
FAU - Choi, Hwan
AU  - Choi H
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea.
FAU - Park, Hanseul
AU  - Park H
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea.
FAU - Cho, Byunguk
AU  - Cho B
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea.
FAU - Kim, Siyoung
AU  - Kim S
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea.
FAU - Kim, Jongpil
AU  - Kim J
AD  - Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South 
      Korea. jk2316@gmail.com.
AD  - Department of Chemistry, Dongguk University, Seoul, 100-715, South Korea. 
      jk2316@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190204
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*genetics
MH  - DNA (Cytosine-5-)-Methyltransferases/*genetics
MH  - DNA Methylation/*genetics
MH  - Epigenesis, Genetic
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Humans
MH  - Mice
MH  - Mouse Embryonic Stem Cells/*cytology/metabolism
MH  - Pluripotent Stem Cells/cytology/metabolism
MH  - DNA Methyltransferase 3B
PMC - PMC6361932
COIS- The authors declare no competing interests.
EDAT- 2019/02/06 06:00
MHDA- 2020/08/21 06:00
PMCR- 2019/02/04
CRDT- 2019/02/06 06:00
PHST- 2018/05/22 00:00 [received]
PHST- 2018/12/04 00:00 [accepted]
PHST- 2019/02/06 06:00 [entrez]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2020/08/21 06:00 [medline]
PHST- 2019/02/04 00:00 [pmc-release]
AID - 10.1038/s41598-018-37372-2 [pii]
AID - 37372 [pii]
AID - 10.1038/s41598-018-37372-2 [doi]
PST - epublish
SO  - Sci Rep. 2019 Feb 4;9(1):1333. doi: 10.1038/s41598-018-37372-2.