PMID- 30719006 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231005 IS - 1664-042X (Print) IS - 1664-042X (Electronic) IS - 1664-042X (Linking) VI - 9 DP - 2018 TI - TREC and KREC Levels as a Predictors of Lymphocyte Subpopulations Measured by Flow Cytometry. PG - 1877 LID - 10.3389/fphys.2018.01877 [doi] LID - 1877 AB - Primary immunodeficiency diseases (PID) is a heterogeneous group of disorders caused by genetic defects of the immune system, which manifests clinically as recurrent infections, autoimmune diseases, or malignancies. Early detection of other PID remains a challenge, particularly in older children due to milder and less specific symptoms, a low level of clinician PID awareness and poor provision of hospital laboratories with appropriate devices. T-cell recombination excision circles (TREC) and kappa-deleting element recombination circle (KREC) in a dried blood spot and in peripheral blood using real-time polymerase chain reaction (PCR) are used as a tool for severe combined immune deficiency but not in PID. They represent an attractive and cheap target for a more extensive use in clinical practice. This study aimed to assess TREC/KREC correspondence with lymphocyte subpopulations, measured by flow cytometry and evaluate correlations between TREC/KREC, lymphocyte subpopulations and immunoglobulins. We carried out analysis of data from children assessed by clinical immunologists at Speransky Children's Hospital, Moscow, Russia with suspected immunodeficiencies between May 2013 and August 2016. Peripheral blood samples were sent for TREC/KREC, flow cytometry (CD3, CD4, CD8, and CD19), IgA, IgM, and IgG analysis. A total of 839 samples were analyzed for using TREC assay and flow cytometry and 931 KREC/flow cytometry. TREC demonstrated an AUC of 0.73 (95% CI 0.70-0.76) for CD3, 0.74 (95% CI 0.71-0.77) for CD4 and 0.67 (95% CI 0.63-0.70) for CD8, respectively, while KREC demonstrated an AUC of 0.72 (95% CI 0.69-0.76) for CD19. Moderate correlation was found between the levels of TREC and CD4 (r = 0.55, p < 0.01) and KREC with CD19 (r = 0.56, p < 0.01). In this study, promising prediction models were tested. We found that TREC and KREC are able to moderately detect abnormal levels of individual lymphocyte subpopulations. Future research should assess associations between TREC/KREC and other lymphocyte subpopulations and approach TREC/KREC use in PID diagnosis. FAU - Korsunskiy, Ilya AU - Korsunskiy I AD - Speransky Children's Hospital, Moscow, Russia. AD - Department of Paediatrics, Sechenov University, Moscow, Russia. AD - Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia. FAU - Blyuss, Oleg AU - Blyuss O AD - Wolfson Institute of Preventive Medicine, Queen Mary University of London, London, United Kingdom. FAU - Gordukova, Maria AU - Gordukova M AD - Speransky Children's Hospital, Moscow, Russia. FAU - Davydova, Nataliia AU - Davydova N AD - Speransky Children's Hospital, Moscow, Russia. AD - Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia. FAU - Gordleeva, Susanna AU - Gordleeva S AD - Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia. FAU - Molchanov, Robert AU - Molchanov R AD - State Institution "Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine", Dnipro, Ukraine. FAU - Asmanov, Alan AU - Asmanov A AD - The Research and Clinical Institute for Pediatrics named after Academician Yuri Veltischev of the Pirogov Russian National Research Medical University, Moscow, Russia. FAU - Peshko, Dmitrii AU - Peshko D AD - Department of Paediatrics, Sechenov University, Moscow, Russia. FAU - Zinovieva, Nataliia AU - Zinovieva N AD - Speransky Children's Hospital, Moscow, Russia. FAU - Zimin, Sergey AU - Zimin S AD - Speransky Children's Hospital, Moscow, Russia. FAU - Lazarev, Vladimir AU - Lazarev V AD - Department of Paediatrics, Sechenov University, Moscow, Russia. FAU - Salpagarova, Aminat AU - Salpagarova A AD - Department of Paediatrics, Sechenov University, Moscow, Russia. FAU - Filipenko, Maxim AU - Filipenko M AD - Pharmacogenomic Laboratory, Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. FAU - Kozlov, Ivan AU - Kozlov I AD - Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia. FAU - Prodeus, Andrey AU - Prodeus A AD - Speransky Children's Hospital, Moscow, Russia. AD - Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia. AD - Immanuel Kant Baltic Federal University, Kaliningrad, Russia. FAU - Korsunskiy, Anatoliy AU - Korsunskiy A AD - Speransky Children's Hospital, Moscow, Russia. AD - Department of Paediatrics, Sechenov University, Moscow, Russia. FAU - Hsu, Peter AU - Hsu P AD - Allergy and Immunology, The Kids Research Institute, The Children's Hospital at Westmead, Sydney, NSW, Australia. AD - The In-VIVO Global Network, An Affiliate of the World Universities Network, New York, NY, United States. FAU - Munblit, Daniel AU - Munblit D AD - Department of Paediatrics, Sechenov University, Moscow, Russia. AD - The In-VIVO Global Network, An Affiliate of the World Universities Network, New York, NY, United States. AD - Department of Paediatrics, Imperial College London, London, United Kingdom. AD - Solov'ev Research and Clinical Center for Neuropsychiatry, Moscow, Russia. LA - eng PT - Journal Article DEP - 20190121 PL - Switzerland TA - Front Physiol JT - Frontiers in physiology JID - 101549006 PMC - PMC6348265 OTO - NOTNLM OT - KREC OT - PID OT - TREC OT - flow cytometry OT - immunoglobulins OT - lymphocyte subpopulations OT - primary immune deficiency EDAT- 2019/02/06 06:00 MHDA- 2019/02/06 06:01 PMCR- 2019/01/21 CRDT- 2019/02/06 06:00 PHST- 2018/09/29 00:00 [received] PHST- 2018/12/11 00:00 [accepted] PHST- 2019/02/06 06:00 [entrez] PHST- 2019/02/06 06:00 [pubmed] PHST- 2019/02/06 06:01 [medline] PHST- 2019/01/21 00:00 [pmc-release] AID - 10.3389/fphys.2018.01877 [doi] PST - epublish SO - Front Physiol. 2019 Jan 21;9:1877. doi: 10.3389/fphys.2018.01877. eCollection 2018.