PMID- 30719459
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2356-6752 (Print)
IS  - 2314-758X (Electronic)
IS  - 2314-758X (Linking)
VI  - 2019
DP  - 2019
TI  - Age-Dependent Protein Expression of Serine/Threonine Phosphatases and Their 
      Inhibitors in the Human Cardiac Atrium.
PG  - 2675972
LID - 10.1155/2019/2675972 [doi]
LID - 2675972
AB  - Heart failure and aging of the heart show many similarities regarding hemodynamic 
      and biochemical parameters. There is evidence that heart failure in experimental 
      animals and humans is accompanied and possibly exacerbated by increased activity 
      of protein phosphatase (PP) 1 and/or 2A. Here, we wanted to study the 
      age-dependent protein expression of major members of the protein phosphatase 
      family in human hearts. Right atrial samples were obtained during bypass surgery. 
      Patients (n=60) were suffering from chronic coronary artery disease (CCS 2-3; New 
      York Heart Association (NYHA) stage 1-3). Age ranged from 48 to 84 years (median 
      69). All patients included in the study were given beta-adrenoceptor blockers. Other 
      medications included angiotensin-converting enzyme (ACE) or 
      angiotensin-receptor-1 (AT(1)) inhibitors, statins, nitrates, and acetylsalicylic 
      acid (ASS). 100 microg of right atrial homogenates was used for western blotting. 
      Antibodies against catalytic subunits (and their major regulatory proteins) of 
      all presently known cardiac serine/threonine phosphatases were used for antigen 
      detection. In detail, we studied the expression of the catalytic subunit of PP1 
      (PP1c); I(1) (PP1) and I(2) (PP1), proteins that can inhibit the activity of 
      PP1c; the catalytic subunit of PP2A (PP2Ac); regulatory A-subunit of PP2A 
      (PP2A(A)); regulatory B56alpha-subunit of PP2A (PP2A(B)); I(1) (PP2A) and I(2) 
      (PP2A), inhibitory subunits of PP2A; catalytic and regulatory subunits of 
      calcineurin: PP2B(A) and PP2B(B); PP2C; PP5; and PP6. All data were obtained 
      within the linear range of the assay. There was a significant decline in PP2Ac 
      and I(2) (PP2A) expression in older patients, whereas all other parameters 
      remained unchanged with age. It remains to be elucidated whether the decrease in 
      the protein expression of I(2) (PP2A) might elevate cardiac PP2A activity in a 
      detrimental way or is overcome by a reduced protein expression and thus a reduced 
      activity of PP2Ac.
FAU - Gergs, Ulrich
AU  - Gergs U
AUID- ORCID: 0000-0001-6986-485X
AD  - Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06112 Halle (Saale), Germany.
FAU - Trapp, Theresa
AU  - Trapp T
AD  - Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06112 Halle (Saale), Germany.
FAU - Bushnaq, Hasan
AU  - Bushnaq H
AD  - Klinik fur Herz- und Thoraxchirurgie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06097 Halle, Germany.
FAU - Simm, Andreas
AU  - Simm A
AD  - Klinik fur Herz- und Thoraxchirurgie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06097 Halle, Germany.
FAU - Silber, Rolf-Edgar
AU  - Silber RE
AD  - Klinik fur Herz- und Thoraxchirurgie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06097 Halle, Germany.
FAU - Neumann, Joachim
AU  - Neumann J
AUID- ORCID: 0000-0001-5018-3851
AD  - Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, 
      Martin-Luther-Universitat Halle-Wittenberg, 06112 Halle (Saale), Germany.
LA  - eng
PT  - Journal Article
DEP - 20190102
PL  - United States
TA  - Adv Med
JT  - Advances in medicine
JID - 101651448
PMC - PMC6334353
EDAT- 2019/02/06 06:00
MHDA- 2019/02/06 06:01
PMCR- 2019/01/02
CRDT- 2019/02/06 06:00
PHST- 2018/02/21 00:00 [received]
PHST- 2018/11/22 00:00 [revised]
PHST- 2018/11/29 00:00 [accepted]
PHST- 2019/02/06 06:00 [entrez]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2019/02/06 06:01 [medline]
PHST- 2019/01/02 00:00 [pmc-release]
AID - 10.1155/2019/2675972 [doi]
PST - epublish
SO  - Adv Med. 2019 Jan 2;2019:2675972. doi: 10.1155/2019/2675972. eCollection 2019.