PMID- 30720102
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20190624
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 41
IP  - 4
DP  - 2019 Apr
TI  - Autophagy and apoptotic machinery caused by Polygonum cuspidatum extract in 
      cisplatin‑resistant human oral cancer CAR cells.
PG  - 2549-2557
LID - 10.3892/or.2019.6985 [doi]
AB  - Polygonum cuspidatum (Hu Zhang) is a traditional Chinese medicine (TCM) and has 
      been revealed to exert anticancer, anti‑angiogenesis, anti‑human immunodeficiency 
      virus (HIV), anti‑hepatitis B virus, anti‑microbial, anti‑inflammatory, and 
      neuro‑protective bio‑activities. However, the effect of P. cuspidatum extract 
      (PCE) on drug‑resistant human oral cancer cells regarding cell death is not fully 
      understood yet. The present study was undertaken to explore the induction of 
      autophagic and apoptotic cell death and to investigate their underlying molecular 
      mechanisms in PCE‑treated cisplatin‑resistant human oral cancer CAR cells. Our 
      results revealed that PCE was determined via HPLC analytic method, and it was 
      revealed that resveratrol may be a major compound in PCE. The data also 
      demonstrated that PCE reduced CAR cell viability in a concentration‑ and 
      time‑dependent response via an MTT assay. PCE had an extremely low toxicity in 
      human normal gingival fibroblasts (HGF). Autophagic and apoptotic cell death was 
      found after PCE treatment by morphological determination. PCE was revealed to 
      induce autophagy as determined using acridine orange (AO), LC3‑GFP, 
      monodansylcadaverine (MDC) and LysoTracker Red staining in CAR cells. In 
      addition, PCE was revealed to induce apoptosis in CAR cells via 
      4',6‑diamidino‑2‑phenylindole (DAPI)/terminal deoxynucleotidyl transferase dUTP 
      nick‑end labeling (TUNEL) double staining. PCE significantly stimulated caspase‑9 
      and ‑3 activities as revealed using caspase activity assays. PCE markedly 
      increased the protein levels of Atg5, Atg7, Atg12, Beclin‑1, LC3, Bax and cleaved 
      caspase‑3, while it decreased the protein expression of Bcl‑2 in CAR cells as 
      determined by western blotting. In conclusion, our findings are the first to 
      suggest that PCE may be potentially efficacious for the treatment of 
      cisplatin‑resistant human oral cancer.
FAU - Wang, Yin-Lai
AU  - Wang YL
AD  - Department of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung 
      80284, Taiwan, R.O.C.
FAU - Horng, Chi-Ting
AU  - Horng CT
AD  - Department of Ophthalmology, Kaohsiung Armed Forces General Hospital, Kaohsiung 
      80284, Taiwan, R.O.C.
FAU - Hsieh, Min-Tsang
AU  - Hsieh MT
AD  - School of Pharmacy, China Medical University, Taichung 40402, Taiwan, R.O.C.
FAU - Chen, Hung-Che
AU  - Chen HC
AD  - Department of Pharmacy and Master Program, Tajen University, Pingtung 90741, 
      Taiwan, R.O.C.
FAU - Huang, Yu-Syuan
AU  - Huang YS
AD  - Department of Pharmacy and Master Program, Tajen University, Pingtung 90741, 
      Taiwan, R.O.C.
FAU - Yang, Jai-Sing
AU  - Yang JS
AD  - Department of Medical Research, China Medical University Hospital, China Medical 
      University, Taichung 40447, Taiwan, R.O.C.
FAU - Wang, Guo-Kai
AU  - Wang GK
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Anhui Key Lab of Modern 
      Chinese Materia Medica, Hefei, Anhui 230012, P.R. China.
FAU - Chiang, Jo-Hua
AU  - Chiang JH
AD  - Department of Nursing, Chung Jen Catholic Junior College, Chiayi 62241, Taiwan, 
      R.O.C.
FAU - Chen, Hul-Han
AU  - Chen HH
AD  - Division of Practice Advancement and Clinical Education, UNC Eshelman School of 
      Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 275297, 
      USA.
FAU - Lu, Chi-Cheng
AU  - Lu CC
AD  - Department of Sport Performance, National Taiwan University of Sport, Taichung 
      40404, Taiwan, R.O.C.
FAU - Chen, Fu-An
AU  - Chen FA
AD  - Department of Pharmacy and Master Program, Tajen University, Pingtung 90741, 
      Taiwan, R.O.C.
LA  - eng
PT  - Journal Article
DEP - 20190128
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Plant Extracts)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology/therapeutic use
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Cell Line, Tumor
MH  - Cisplatin/*pharmacology/therapeutic use
MH  - Drug Resistance, Neoplasm
MH  - Drug Screening Assays, Antitumor
MH  - Fallopia japonica/*chemistry
MH  - Humans
MH  - Mouth Neoplasms/*drug therapy/pathology
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Signal Transduction/drug effects
EDAT- 2019/02/06 06:00
MHDA- 2019/06/25 06:00
CRDT- 2019/02/06 06:00
PHST- 2018/09/28 00:00 [received]
PHST- 2019/01/04 00:00 [accepted]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2019/02/06 06:00 [entrez]
AID - 10.3892/or.2019.6985 [doi]
PST - ppublish
SO  - Oncol Rep. 2019 Apr;41(4):2549-2557. doi: 10.3892/or.2019.6985. Epub 2019 Jan 28.