PMID- 30720112
OWN - NLM
STAT- MEDLINE
DCOM- 20190816
LR  - 20211209
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 54
IP  - 4
DP  - 2019 Apr
TI  - Upregulation of microRNA‑194‑5p inhibits hypopharyngeal carcinoma cell 
      proliferation, migration and invasion by targeting SMURF1 via the mTOR signaling 
      pathway.
PG  - 1245-1255
LID - 10.3892/ijo.2019.4711 [doi]
AB  - Hypopharyngeal carcinoma (HPC) is an aggressive malignancy with the worst 
      prognosis among all head and neck cancers. MicroRNAs (miRNAs) are involved in the 
      development of many human cancers, and may function as oncogenes or tumor 
      suppressors. The present study aimed to evaluate the effects of miRNA 
      (miR)‑194‑5p on the proliferation and invasion of HPC cells and to identify the 
      potential regulatory mechanism. First, miR‑194‑5p and Smad ubiquitin regulatory 
      factor 1 (SMURF1) expression levels were examined in HPC tissues. Subsequently, 
      to explore the effects of miR‑194‑5p on SMURF1, a dual‑luciferase reporter gene 
      assay was performed to verify the target relationship. To define the role of 
      miR‑194‑5p in HPC progression, miR‑194‑5p upregulation and depletion were used to 
      evaluate its effects on cell viability, invasion and migration. SMURF1 silencing 
      and rapamycin [an inhibitor of the mammalian target of rapamycin (mTOR) signaling 
      pathway] treatment were also used to analyze the regulatory mechanism in HPC. 
      Finally, tumor growth was assessed in xenografted tumors in nude mice. SMURF1 was 
      demonstrated to be highly expressed, whereas miR‑194‑5p was poorly expressed in 
      HPC tissues; SMURF1 was identified as a target gene of miR‑194‑5p. FaDu 
      hypopharyngeal squamous cell carcinoma cells treated with miR‑194‑5p mimics 
      exhibited decreased viability, invasion and migration. The results indicated that 
      miR‑194‑5p may inactivate the mTOR signaling pathway by targeting SMURF1. In 
      addition, the in vivo experiments further verified these regulatory effects. 
      These data suggested that miR‑194‑5p‑targeted SMURF1 inhibition may be involved 
      in the disruption of HPC progression through the repression of the mTOR signaling 
      pathway.
FAU - Xu, Shan
AU  - Xu S
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Hui, Lian
AU  - Hui L
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Yang, Ning
AU  - Yang N
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Zhao, Ning
AU  - Zhao N
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Jiang, Xue-Jun
AU  - Jiang XJ
AD  - Department of Ear, Nose and Throat, The First Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190204
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN194 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.3.2.26 (SMURF1 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
MH  - 3' Untranslated Regions
MH  - Aged
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Hypopharyngeal Neoplasms/genetics/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Transplantation
MH  - Signal Transduction
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Ubiquitin-Protein Ligases/*genetics
MH  - *Up-Regulation
PMC - PMC6411369
OTO - NOTNLM
OT  - microRNA-194-5p
OT  - hypopharyngeal carcinoma
OT  - Smad ubiquitin regulatory factor 1
OT  - mammalian target of rapamycin signaling pathway
OT  - proliferation
OT  - metastasis
EDAT- 2019/02/06 06:00
MHDA- 2019/08/17 06:00
PMCR- 2019/02/04
CRDT- 2019/02/06 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/11/23 00:00 [accepted]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2019/08/17 06:00 [medline]
PHST- 2019/02/06 06:00 [entrez]
PHST- 2019/02/04 00:00 [pmc-release]
AID - ijo-54-04-1245 [pii]
AID - 10.3892/ijo.2019.4711 [doi]
PST - ppublish
SO  - Int J Oncol. 2019 Apr;54(4):1245-1255. doi: 10.3892/ijo.2019.4711. Epub 2019 Feb 
      4.