PMID- 30720173
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20221207
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 2
DP  - 2019 Jan
TI  - Reduced miR-363-3p expression in non-small cell lung cancer is associated with 
      gemcitabine resistance via targeting of CUL4A.
PG  - 649-659
LID - 16879 [pii]
LID - 10.26355/eurrev_201901_16879 [doi]
AB  - OBJECTIVE: Accumulating evidence has suggested that aberrant expression of 
      microRNAs (miRNAs) is associated with non-small cell lung cancer (NSCLC) 
      proliferation, migration, invasion and chemotherapy resistance. Cullin4A (CUL4A) 
      has been previously reported to desensitize NSCLC cells to chemotherapy 
      treatment. However, whether miRNAs regulate CUL4A to promote chemotherapy 
      resistance remains unknown. PATIENTS AND METHODS: Tissues were obtained from 40 
      NSCLC patients who received surgery at the Yancheng City No. 1 People's Hospital. 
      Cell Counting Kit-8 (CCK-8) assays were applied for the detection of cell 
      proliferation; mRNA and protein levels were determined by Real Time-quantitative 
      Polymerase Chain Reaction (RT-qPCR) and Western blot, respectively. The 
      interaction between mRNA 3'UTR and miRNA was predicted by TargetScan and verified 
      by Dual-Luciferase reporter assay. RESULTS: In the present study, miR-363-3p 
      levels were revealed to be significantly decreased in tumor tissues obtained from 
      NSCLC patients compared with adjacent normal tissues. The results of the CCK-8 
      assays showed that the overexpression of miR-363-3p may slightly inhibit the 
      proliferation of A549 and H23 cells. Notably, the transfection with miR-363-3p 
      antagonists reduced the sensitivity of A549 and H23 cells to gemcitabine 
      treatment, whereas the overexpression of miR-363-3p markedly increased the 
      sensitivity of A549 and H23 cells to gemcitabine treatment. Furthermore, CUL4A 
      mRNA and protein levels were revealed to be decreased in A549 cells transfected 
      with miR-363-3p mimics. The Dual-Luciferase reporter assay results further 
      suggested that CUL4A represents a target gene of miR-363-3p. CONCLUSIONS: The 
      results indicated that decreased miR-363-3p expression enhanced gemcitabine 
      resistance in NSCLC cells via regulation of CUL4A.
FAU - Bian, W-G
AU  - Bian WG
AD  - Department of Oncology, Yancheng City No. 1 People's Hospital, Yancheng, China. 
      chenpingycph@yeah.net.
FAU - Zhou, X-N
AU  - Zhou XN
FAU - Song, S
AU  - Song S
FAU - Chen, H-T
AU  - Chen HT
FAU - Shen, Y
AU  - Shen Y
FAU - Chen, P
AU  - Chen P
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (MIRN363 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0W860991D6 (Deoxycytidine)
RN  - 0 (Gemcitabine)
SB  - IM
RIN - Eur Rev Med Pharmacol Sci. 2021 Nov;25(21):6444. PMID: 34787845
MH  - A549 Cells
MH  - Carcinoma, Non-Small-Cell Lung/genetics/pathology/*therapy
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation/genetics
MH  - Chemotherapy, Adjuvant
MH  - Cullin Proteins/*genetics
MH  - Deoxycytidine/*analogs & derivatives/pharmacology/therapeutic use
MH  - Down-Regulation
MH  - Drug Resistance, Neoplasm/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lung/pathology/surgery
MH  - Lung Neoplasms/genetics/pathology/*therapy
MH  - MicroRNAs/antagonists & inhibitors/*metabolism
MH  - Pneumonectomy
MH  - Gemcitabine
EDAT- 2019/02/06 06:00
MHDA- 2020/07/21 06:00
CRDT- 2019/02/06 06:00
PHST- 2019/02/06 06:00 [entrez]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
AID - 16879 [pii]
AID - 10.26355/eurrev_201901_16879 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):649-659. doi: 
      10.26355/eurrev_201901_16879.