PMID- 30720190
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20200915
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 2
DP  - 2019 Jan
TI  - Influence of MiR-154 on myocardial apoptosis in rats with acute myocardial 
      infarction through Wnt/beta-catenin signaling pathway.
PG  - 818-825
LID - 16896 [pii]
LID - 10.26355/eurrev_201901_16896 [doi]
AB  - OBJECTIVE: To explore the influence of micro ribonucleic acid (miR)-154 on 
      myocardial apoptosis in rats with acute myocardial infarction (AMI), and to 
      analyze whether Wnt/beta-catenin signaling pathway was involved in the regulation. 
      MATERIALS AND METHODS: The Sprague-Dawley (SD) rat model of AMI was established 
      via ligation of left anterior descending artery. Rats were randomly divided into 
      model group (M group, n=12) and ICG-001 intervention group (I group, n=12). At 
      the same time, sham operation group (S group, n=12) was established. In I group, 
      ICG-001 (5 mg/kg) was intraperitoneally injected every day after operation. 
      Meanwhile, an equal amount of normal saline was injected in rats of S group and M 
      group. 21 d after operation, the cardiac function of rats in each group was 
      detected via echocardiography. After that, the rats were immediately executed. MI 
      area in each group was detected via 2,3,5-triphenyltetrazolium chloride (TTC) 
      staining. Myocardial apoptosis level in each group was detected via terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. 
      Moreover, the changes of apoptotic proteins in rat myocardial cells were detected 
      via Western blotting. Moreover, the expression level of miR-154 in myocardial 
      cells of rats was detected via quantitative polymerase chain reaction (qPCR). 
      Furthermore, the influence of miR-154 on Wnt/beta-catenin signaling pathway was 
      detected via Western blotting. RESULTS: Compared with S group, left ventricular 
      ejection fraction (LVEF, %) and left ventricular fractional shortening (LVFS, %) 
      were significantly decreased in M group (p<0.01). However, left ventricular 
      internal diameter at end-diastole (LVIDd) and left ventricular internal diameter 
      at end-systole (LVIDs) were significantly increased (p<0.01). In I group, LVEF 
      (%) and LVFS (%) were significantly higher than those of M group (p<0.05), 
      whereas LVIDs and LVIDd were significantly lower (p<0.05). MI area in M group was 
      remarkably larger than that of S group (p<0.01). Meanwhile, MI area in I group 
      was significantly smaller than that of M group (p<0.01). Compared with S group, 
      the number of apoptotic myocardial cells and the protein expression level of 
      cleaved caspase-3 were significantly increased in M group (p<0.01). However, the 
      expression level of B-cell lymphoma-2/Bcl-2 associated X protein (Bcl-2/Bax) was 
      significantly decreased (p<0.01). The number of apoptotic myocardial cells and 
      the protein expression level of cleaved caspase-3 were significantly declined in 
      I group when compared with those of M group (p<0.01). However, the expression 
      level of Bcl-2/Bax was significantly increased in I group (p<0.01). The 
      expression level of miR-154 in myocardial cells of M group and I group was 
      remarkably increased when compared with that of S group (p<0.01). Furthermore, 
      the expression levels of beta-catenin and Cyclin D1 in myocardial cells of M group 
      were remarkably higher than those of S group and I group (p<0.01). CONCLUSIONS: 
      AMI significantly increases the expression level of miR-154. Moreover, miR-154 
      can activate Wnt/beta-catenin signaling pathway, eventually promoting myocardial 
      apoptosis.
FAU - Sun, H-Y
AU  - Sun HY
AD  - Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical 
      University, Xinxiang, China. guoanzhao@xxmu.edu.cn.
FAU - Wang, X-L
AU  - Wang XL
FAU - Ma, L-C
AU  - Ma LC
FAU - Yang, M
AU  - Yang M
FAU - Yang, H-J
AU  - Yang HJ
FAU - Huang, H-W
AU  - Huang HW
FAU - Zhao, G-A
AU  - Zhao GA
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Ctnnb1 protein, rat)
RN  - 0 (ICG 001)
RN  - 0 (MIRN154 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Pyrimidinones)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Bridged Bicyclo Compounds, Heterocyclic/administration & dosage
MH  - Disease Models, Animal
MH  - MicroRNAs/*metabolism
MH  - Myocardial Infarction/diagnosis/drug therapy/*genetics/pathology
MH  - Myocardium/cytology/*pathology
MH  - Myocytes, Cardiac/*pathology
MH  - Pyrimidinones/administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stroke Volume/drug effects/genetics
MH  - Ventricular Function, Left/drug effects/genetics
MH  - Wnt Proteins/antagonists & inhibitors/metabolism
MH  - Wnt Signaling Pathway/drug effects/*genetics
MH  - beta Catenin/metabolism
EDAT- 2019/02/06 06:00
MHDA- 2020/07/21 06:00
CRDT- 2019/02/06 06:00
PHST- 2019/02/06 06:00 [entrez]
PHST- 2019/02/06 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
AID - 16896 [pii]
AID - 10.26355/eurrev_201901_16896 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):818-825. doi: 
      10.26355/eurrev_201901_16896.