PMID- 30722983
OWN - NLM
STAT- MEDLINE
DCOM- 20190617
LR  - 20190617
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 45
IP  - 3
DP  - 2019 Mar
TI  - Healing Capacity of Autologous Bone Marrow-derived Mesenchymal Stem Cells on 
      Partially Pulpotomized Dogs' Teeth.
PG  - 287-294
LID - S0099-2399(18)30826-4 [pii]
LID - 10.1016/j.joen.2018.11.013 [doi]
AB  - INTRODUCTION: Partial pulpotomy is a vital treatment for carious and traumatic 
      exposure, especially in young permanent teeth. Cell-based therapy for partially 
      pulpotomized teeth can be considered a promising approach for dentin-pulp complex 
      regeneration. This study evaluated the healing capacity of autologous bone 
      marrow-derived stem cells (BMSCs) on partially pulpotomized teeth in a dog model. 
      METHODS: Twelve mongrel dogs were selected, and a total number of 192 posterior 
      mandibular and maxillary teeth were involved in the study (16 teeth per dog). 
      Partial pulpotomies were performed, and the dogs were assigned into 2 equal 
      groups (groups 1 and 2), 6 dogs in each group. The coronal pulp cavities (n = 96) 
      of group 1 were filled with calcium silicate-based capping material. Group 2 
      coronal pulp cavities (n = 96) were seeded with 1 x 105 cell/mL BMSCs, and then 
      the cavities were filled with calcium silicate-based capping material. After 
      placing the capping materials, the cavities of both groups (1 and 2) were filled 
      with resin-modified glass ionomer restorative material. From each group, 48 teeth 
      from 3 dogs were evaluated histologically after 1 week, and the other 48 teeth 
      from the remaining 3 dogs were evaluated after 9 weeks. Scoring was done for the 
      amount of inflammatory cell infiltrates, tissue necrosis, and thickness of hard 
      tissue bridge formation. RESULTS: The Mann-Whitney U statistical test performed 
      for hard tissue bridge formation revealed significant differences between the 2 
      groups at the 1- (P < .05) and 9-week (P < .05) examination periods. CONCLUSIONS: 
      Autologous BMSCs have significant therapeutic potential because they enhance the 
      healing capacity of partially pulpotomized dogs' teeth.
CI  - Copyright (c) 2018 American Association of Endodontists. Published by Elsevier Inc. 
      All rights reserved.
FAU - El-Zekrid, Mona H
AU  - El-Zekrid MH
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, 
      Egypt. Electronic address: monazekrd@gmail.com.
FAU - Mahmoud, Salah H
AU  - Mahmoud SH
AD  - Department of Conservative Dentistry, Faculty of Dentistry, Mansoura University, 
      Mansoura, Egypt.
FAU - Ali, Fawzy A
AU  - Ali FA
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, 
      Egypt.
FAU - Helal, Mohamed E
AU  - Helal ME
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, 
      Egypt.
FAU - Grawish, Mohammed E
AU  - Grawish ME
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, 
      Egypt; Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta 
      University for Science and Technology, Gamasa, Mansoura, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20190202
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Calcium Compounds)
RN  - 0 (Pulp Capping and Pulpectomy Agents)
RN  - 0 (Silicates)
RN  - S4255P4G5M (calcium silicate)
MH  - Animals
MH  - Autografts
MH  - Bone Marrow Cells/*cytology
MH  - Calcium Compounds/therapeutic use
MH  - *Dental Pulp Capping
MH  - Dogs
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Pulp Capping and Pulpectomy Agents/therapeutic use
MH  - *Pulpotomy
MH  - Silicates/therapeutic use
MH  - Tooth/*physiology/physiopathology/*surgery
MH  - *Wound Healing
OTO - NOTNLM
OT  - Bone marrow-derived mesenchymal stem cells
OT  - dog teeth
OT  - histologic staining
OT  - partial pulpotomy
OT  - regenerative endodontic
EDAT- 2019/02/07 06:00
MHDA- 2019/06/18 06:00
CRDT- 2019/02/07 06:00
PHST- 2018/07/09 00:00 [received]
PHST- 2018/11/03 00:00 [revised]
PHST- 2018/11/25 00:00 [accepted]
PHST- 2019/02/07 06:00 [pubmed]
PHST- 2019/06/18 06:00 [medline]
PHST- 2019/02/07 06:00 [entrez]
AID - S0099-2399(18)30826-4 [pii]
AID - 10.1016/j.joen.2018.11.013 [doi]
PST - ppublish
SO  - J Endod. 2019 Mar;45(3):287-294. doi: 10.1016/j.joen.2018.11.013. Epub 2019 Feb 
      2.