PMID- 30724629
OWN - NLM
STAT- MEDLINE
DCOM- 20190222
LR  - 20200225
IS  - 1475-6374 (Electronic)
IS  - 1475-6366 (Print)
IS  - 1475-6366 (Linking)
VI  - 34
IP  - 1
DP  - 2019 Dec
TI  - Evaluation of a flavonoids library for inhibition of pancreatic alpha-amylase towards 
      a structure-activity relationship.
PG  - 577-588
LID - 10.1080/14756366.2018.1558221 [doi]
AB  - alpha-Amylase has been considered an important therapeutic target for the management 
      of type 2 diabetes mellitus (T2DM), decreasing postprandial hyperglycaemia 
      (PPHG). In the present work, a panel of 40 structurally related flavonoids was 
      tested, concerning their ability to inhibit alpha-amylase activity, using a 
      microanalysis screening system, an inhibitory kinetic analysis and molecular 
      docking calculations. From the obtained results, it was possible to observe that 
      the flavone with a -Cl ion at 3-position of C-ring, an -OH group at 3'- and 4'- 
      positions of B-ring and at 5- and 7- positions of A-ring and the C2 = C3 double 
      bond, was the most active tested flavonoid, through competitive inhibition. In 
      conclusion, some of the tested flavonoids have shown promising inhibition of 
      alpha-amylase and may be considered as possible alternatives to the modulation of 
      T2DM.
FAU - Proenca, Carina
AU  - Proenca C
AUID- ORCID: 0000-0003-0859-6526
AD  - a LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical 
      Sciences, Faculty of Pharmacy , University of Porto , Porto , Portugal.
FAU - Freitas, Marisa
AU  - Freitas M
AUID- ORCID: 0000-0001-9114-9967
AD  - a LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical 
      Sciences, Faculty of Pharmacy , University of Porto , Porto , Portugal.
FAU - Ribeiro, Daniela
AU  - Ribeiro D
AUID- ORCID: 0000-0001-9733-7934
AD  - a LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical 
      Sciences, Faculty of Pharmacy , University of Porto , Porto , Portugal.
FAU - Tome, Sara M
AU  - Tome SM
AUID- ORCID: 0000-0002-9021-2909
AD  - b Department of Chemistry and QOPNA , University of Aveiro , Aveiro , Portugal.
FAU - Oliveira, Eduardo F T
AU  - Oliveira EFT
AUID- ORCID: 0000-0003-4637-2099
AD  - c UCIBIO, REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences 
      , University of Porto , Porto , Portugal.
FAU - Viegas, Matilde F
AU  - Viegas MF
AUID- ORCID: 0000-0002-4472-9691
AD  - c UCIBIO, REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences 
      , University of Porto , Porto , Portugal.
FAU - Araujo, Alberto N
AU  - Araujo AN
AUID- ORCID: 0000-0001-5348-4924
AD  - a LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical 
      Sciences, Faculty of Pharmacy , University of Porto , Porto , Portugal.
FAU - Ramos, Maria J
AU  - Ramos MJ
AUID- ORCID: 0000-0002-7554-8324
AD  - c UCIBIO, REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences 
      , University of Porto , Porto , Portugal.
FAU - Silva, Artur M S
AU  - Silva AMS
AUID- ORCID: 0000-0003-2861-8286
AD  - b Department of Chemistry and QOPNA , University of Aveiro , Aveiro , Portugal.
FAU - Fernandes, Pedro A
AU  - Fernandes PA
AUID- ORCID: 0000-0003-2748-4722
AD  - c UCIBIO, REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences 
      , University of Porto , Porto , Portugal.
FAU - Fernandes, Eduarda
AU  - Fernandes E
AUID- ORCID: 0000-0001-6424-0976
AD  - a LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical 
      Sciences, Faculty of Pharmacy , University of Porto , Porto , Portugal.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Enzyme Inhib Med Chem
JT  - Journal of enzyme inhibition and medicinal chemistry
JID - 101150203
RN  - 0 (Flavonoids)
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Small Molecule Libraries)
RN  - EC 3.2.1.1 (Pancreatic alpha-Amylases)
SB  - IM
MH  - Dose-Response Relationship, Drug
MH  - Flavonoids/chemical synthesis/chemistry/*pharmacology
MH  - Glycoside Hydrolase Inhibitors/chemical synthesis/chemistry/*pharmacology
MH  - Humans
MH  - Molecular Structure
MH  - Pancreatic alpha-Amylases/*antagonists & inhibitors/metabolism
MH  - Small Molecule Libraries/chemical synthesis/chemistry/*pharmacology
MH  - Structure-Activity Relationship
PMC - PMC6366418
OTO - NOTNLM
OT  - diabetes
OT  - docking
OT  - flavonoids
OT  - alpha-Amylase inhibition
EDAT- 2019/02/07 06:00
MHDA- 2019/02/23 06:00
PMCR- 2019/02/06
CRDT- 2019/02/07 06:00
PHST- 2019/02/07 06:00 [entrez]
PHST- 2019/02/07 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2019/02/06 00:00 [pmc-release]
AID - 1558221 [pii]
AID - 10.1080/14756366.2018.1558221 [doi]
PST - ppublish
SO  - J Enzyme Inhib Med Chem. 2019 Dec;34(1):577-588. doi: 
      10.1080/14756366.2018.1558221.