PMID- 30730243
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20200518
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 39
IP  - 4
DP  - 2019 Apr
TI  - MCP-1/CCL2 Mediated by Autocrine Loop of PDGF-BB Promotes Invasion of Lung Cancer 
      Cell by Recruitment of Macrophages Via CCL2-CCR2 Axis.
PG  - 224-232
LID - 10.1089/jir.2018.0113 [doi]
AB  - Platelet-derived growth factor-BB (PDGF-BB) is recognized as a potential player 
      in a paracrine manner in tumor stroma development. PDGF-BB has an autocrine 
      growth function in lung cancer cells; however, the mechanism in nonsmall-cell 
      lung cancer is not fully understood. In this study, we report that PDGF-BB 
      increased monocyte chemoattractant protein-1 (MCP-1)-dependent macrophage 
      recruitment and that expression of metastatic genes increased in A549 cells 
      cocultured with RAW 264.7 macrophages. Similar to exogenous PDGF-BB, PDGF-BB 
      might have a self-stimulation in invasion of cancer cells during reciprocal 
      activation of cancer cells and tumor-associated macrophages through secretion of 
      soluble proteins. Also, we found that PDGF-BB upregulates both mRNA and protein 
      level of MCP-1 expression in human A549 cells through mitogen-activated protein 
      kinases and phosphatidylinositol 3-kinase/Akt cell signaling pathways, binding 
      NFkappaB to MCP-1 promoter site and PDGF-Rbeta as critical receptor. These results 
      suggested that MCP-1/chemokine (C-C motif) ligand 2 (CCL2) expression mediated by 
      the autocrine loop of PDGF-BB enhanced recruitment of macrophages through 
      CCL2-CCR2 axis, which could ultimately increase expression of metastatic genes in 
      lung cancer cells, finally promoting invasive potential of cancer cells.
FAU - Ding, Mei
AU  - Ding M
AD  - Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 
      P. R. China.
FAU - He, Shao-Jun
AU  - He SJ
AD  - Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 
      P. R. China.
FAU - Yang, Jiong
AU  - Yang J
AD  - Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 
      P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190207
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 1B56C968OA (Becaplermin)
SB  - IM
MH  - Animals
MH  - *Autocrine Communication
MH  - Becaplermin/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Humans
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Macrophages/*metabolism
MH  - Mice
MH  - RAW 264.7 Cells
MH  - Receptors, CCR2/*metabolism
OTO - NOTNLM
OT  - MCP-1 (CCL2)
OT  - PDGF-BB
OT  - invasion
OT  - lung cancer
OT  - macrophages
EDAT- 2019/02/08 06:00
MHDA- 2020/05/19 06:00
CRDT- 2019/02/08 06:00
PHST- 2019/02/08 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2019/02/08 06:00 [entrez]
AID - 10.1089/jir.2018.0113 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2019 Apr;39(4):224-232. doi: 10.1089/jir.2018.0113. 
      Epub 2019 Feb 7.