PMID- 30733642
OWN - NLM
STAT- MEDLINE
DCOM- 20190808
LR  - 20200225
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2019
DP  - 2019
TI  - Chemokines and Growth Factors Produced by Lymphocytes in the Incompetent Great 
      Saphenous Vein.
PG  - 7057303
LID - 10.1155/2019/7057303 [doi]
LID - 7057303
AB  - The role of cytokines in the pathogenesis of chronic venous disease (CVD) remains 
      obscure. It has been postulated that oscillatory flow present in incompetent 
      veins causes proinflammatory changes. Our earlier study confirmed this 
      hypothesis. This study is aimed at assessing chemokines and growth factors (GFs) 
      released by lymphocytes in patients with great saphenous vein (GSV) incompetence. 
      In 34 patients exhibiting reflux in GSV, blood was derived from the cubital vein 
      and from the incompetent saphenofemoral junction. In 12 healthy controls, blood 
      was derived from the cubital vein. Lymphocyte culture with and without 
      stimulation by phytohemagglutinin (PHA) was performed. Eotaxin, interleukin 8 
      (IL-8), macrophage inflammatory protein 1 A and 1B (MIP-1A and MIP-1B), 
      interferon gamma-induced protein (IP-10), monocyte chemoattractant protein-1 
      (MCP-1), interleukin 5 (IL-5), fibroblast growth factor (FGF), granulocyte 
      colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating 
      factor (GM-CSF), platelet-derived growth factor-BB (PDGF-BB), and vascular 
      endothelial growth factor (VEGF) were assessed in culture supernatants by a 
      Bio-Plex assay. Higher concentrations of eotaxin and G-CSF were revealed in the 
      incompetent GSV, compared with the concentrations in the patients' upper limbs. 
      The concentrations of MIP-1A and MIP-1B were higher in the CVD group while the 
      concentration of VEGF was lower. In the stimulated cultures, the concentration of 
      G-CSF proved higher in the incompetent GSV, as compared with the patients' upper 
      limbs. Between the groups, the concentration of eotaxin was higher in the CVD 
      group, while the IL-5 and MCP-1 concentrations were lower. IL-8, IP-10, FGF, 
      GM-CSF, and PDGF-BB did not reveal any significant differences in concentrations 
      between the samples. These observations suggest that the concentrations of 
      chemokines and GFs are different in the blood of CVD patients. The oscillatory 
      flow present in incompetent veins may play a role in these changes. However, the 
      role of cytokines in CVD requires further study.
FAU - Grudzinska, Ewa
AU  - Grudzinska E
AUID- ORCID: 0000-0003-2283-0286
AD  - School of Medicine with the Division of Dentistry in Zabrze, Medical University 
      of Silesia in Katowice, Department of Microbiology and Immunology, Jordana 19, 
      41-808 Zabrze, Poland.
FAU - Grzegorczyn, Slawomir
AU  - Grzegorczyn S
AD  - School of Medicine with the Division of Dentistry in Zabrze, Medical University 
      of Silesia in Katowice, Department of Biophysics, Jordana 19, 41-808 Zabrze, 
      Poland.
FAU - Czuba, Zenon P
AU  - Czuba ZP
AUID- ORCID: 0000-0001-8216-4495
AD  - School of Medicine with the Division of Dentistry in Zabrze, Medical University 
      of Silesia in Katowice, Department of Microbiology and Immunology, Jordana 19, 
      41-808 Zabrze, Poland.
LA  - eng
PT  - Journal Article
DEP - 20190110
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Chemokines)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
MH  - Adult
MH  - Aged
MH  - Cardiovascular Diseases/metabolism
MH  - Chemokines/*blood
MH  - Female
MH  - Humans
MH  - Immune System
MH  - Inflammation
MH  - Intercellular Signaling Peptides and Proteins/*blood
MH  - Lymphocytes/*cytology
MH  - Male
MH  - Middle Aged
MH  - Neovascularization, Physiologic
MH  - Oscillometry
MH  - Saphenous Vein/*metabolism
MH  - Vascular Diseases/genetics/pathology
MH  - Young Adult
PMC - PMC6348837
EDAT- 2019/02/09 06:00
MHDA- 2019/08/09 06:00
PMCR- 2019/01/10
CRDT- 2019/02/09 06:00
PHST- 2018/10/04 00:00 [received]
PHST- 2018/11/20 00:00 [revised]
PHST- 2018/11/28 00:00 [accepted]
PHST- 2019/02/09 06:00 [entrez]
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2019/08/09 06:00 [medline]
PHST- 2019/01/10 00:00 [pmc-release]
AID - 10.1155/2019/7057303 [doi]
PST - epublish
SO  - Mediators Inflamm. 2019 Jan 10;2019:7057303. doi: 10.1155/2019/7057303. 
      eCollection 2019.