PMID- 30733838
OWN - NLM
STAT- MEDLINE
DCOM- 20190529
LR  - 20200225
IS  - 1875-8630 (Electronic)
IS  - 0278-0240 (Print)
IS  - 0278-0240 (Linking)
VI  - 2019
DP  - 2019
TI  - DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 
      Signaling Pathway in Laryngeal Squamous Cell Carcinoma.
PG  - 6716472
LID - 10.1155/2019/6716472 [doi]
LID - 6716472
AB  - Cancer bioinformatics has been used to screen possible key cancer genes and 
      pathways. Here, through bioinformatics analysis, we found that high expression of 
      diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in 
      head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma 
      (LSCC). The effect of DIAPH1 in LSCC has not been previously investigated. 
      Therefore, we evaluated the expression, function, and molecular mechanisms of 
      DIAPH1 in LSCC. Immunohistochemistry and western blot analysis confirmed the 
      significant upregulation of DIAPH1 in LSCC. We used DIAPH1 RNA interference to 
      construct two DIAPH1-knockdown LSCC cell lines, AMC-HN-8 and FD-LSC-1, and 
      validated the knockdown efficiency. Flow cytometry data showed that DIAPH1 
      inhibited apoptosis. Further, western blot analysis revealed that DIAPH1 
      knockdown increased the protein levels of ATR, p-p53, Bax, and cleaved caspase-3, 
      -8, and -9. Thus, DIAPH1 is upregulated in LSCC and may act as an oncogene by 
      inhibiting apoptosis through the ATR/p53/caspase-3 pathway in LSCC cells.
FAU - Yang, Jiechao
AU  - Yang J
AUID- ORCID: 0000-0002-5426-383X
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Third People's 
      Hospital, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Zhou, Liang
AU  - Zhou L
AUID- ORCID: 0000-0002-8588-2959
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Zhang, Yanping
AU  - Zhang Y
AD  - Laboratory of Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, 
      China.
FAU - Zheng, Juan
AU  - Zheng J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Zhou, Jian
AU  - Zhou J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Wei, Zheqiang
AU  - Wei Z
AD  - Department of Pathology, The Third People's Hospital, Affiliated Hospital of 
      Jiangnan University, Wuxi, Jiangsu, China.
FAU - Zou, Jiaping
AU  - Zou J
AUID- ORCID: 0000-0001-9864-6464
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Third People's 
      Hospital, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20190114
PL  - United States
TA  - Dis Markers
JT  - Disease markers
JID - 8604127
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DIAPH1 protein, human)
RN  - 0 (Formins)
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.1 (ATR protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics/metabolism
MH  - *Apoptosis
MH  - Ataxia Telangiectasia Mutated Proteins/metabolism
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Formins
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Middle Aged
MH  - *Signal Transduction
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Up-Regulation
PMC - PMC6348834
EDAT- 2019/02/09 06:00
MHDA- 2019/05/30 06:00
PMCR- 2019/01/14
CRDT- 2019/02/09 06:00
PHST- 2018/07/29 00:00 [received]
PHST- 2018/10/07 00:00 [revised]
PHST- 2018/11/06 00:00 [accepted]
PHST- 2019/02/09 06:00 [entrez]
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2019/05/30 06:00 [medline]
PHST- 2019/01/14 00:00 [pmc-release]
AID - 10.1155/2019/6716472 [doi]
PST - epublish
SO  - Dis Markers. 2019 Jan 14;2019:6716472. doi: 10.1155/2019/6716472. eCollection 
      2019.