PMID- 30734124
OWN - NLM
STAT- MEDLINE
DCOM- 20190718
LR  - 20190718
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 103
IP  - 7
DP  - 2019 Apr
TI  - Biocontrol of Brettanomyces/Dekkera bruxellensis in alcoholic fermentations using 
      saccharomycin-overproducing Saccharomyces cerevisiae strains.
PG  - 3073-3083
LID - 10.1007/s00253-019-09657-7 [doi]
AB  - Microbial contamination of alcoholic fermentation processes (e.g. winemaking and 
      fuel-ethanol production) is a serious problem for the industry since it may 
      render the product unacceptable and/or reduce its productivity, leading to large 
      economic losses. Brettanomyces/Dekkera bruxellensis is one of the most dangerous 
      microbial contaminant of ethanol industrial fermentations. In the case of wine, 
      this yeast species can produce phenolic compounds that confer off-flavours to the 
      final product. In fuel-ethanol fermentations, D. bruxellensis is a persistent 
      contaminant that affects ethanol yields and productivities. We recently found 
      that Saccharomyces cerevisiae secretes a biocide, which we named saccharomycin, 
      composed of antimicrobial peptides (AMPs) derived from the glycolytic enzyme 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Saccharomycin is active against 
      several wine-related yeast species, namely D. bruxellensis. However, the levels 
      of saccharomycin naturally secreted by S. cerevisiae during alcoholic 
      fermentation are not sufficient to ensure the complete death of D. bruxellensis. 
      Therefore, the aim of the present work was to construct genetically modified S. 
      cerevisiae strains to overproduce these GAPDH-derived AMPs. The expression levels 
      of the nucleotides sequences encoding the AMPs were evaluated in the modified S. 
      cerevisiae strains by RT-qPCR, confirming the success of the recombinant 
      approach. Furthermore, we confirmed by immunological tests that the modified S. 
      cerevisiae strains secreted higher amounts of the AMPs by comparison with the 
      non-modified strain, inducing total death of D. bruxellensis during alcoholic 
      fermentations.
FAU - Branco, Patricia
AU  - Branco P
AD  - Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior 
      de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
AD  - Unit of Bioenergy, Laboratorio Nacional de Energia e Geologia (LNEG), Estrada do 
      Paco do Lumiar 22, 1649-038, Lisbon, Portugal.
FAU - Sabir, Farzana
AU  - Sabir F
AD  - Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior 
      de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
AD  - Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, 
      Universidade de Lisboa, 1649-003, Lisbon, Portugal.
FAU - Diniz, Mario
AU  - Diniz M
AD  - UCIBIO-REQUIMTE, Department of Chemistry, Faculdade de Ciencias e Tecnologia, 
      Universidade NOVA de Lisboa, 2829-516, Caparica, Portugal.
FAU - Carvalho, Luisa
AU  - Carvalho L
AD  - Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior 
      de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
FAU - Albergaria, Helena
AU  - Albergaria H
AUID- ORCID: 0000-0002-9062-9827
AD  - Unit of Bioenergy, Laboratorio Nacional de Energia e Geologia (LNEG), Estrada do 
      Paco do Lumiar 22, 1649-038, Lisbon, Portugal. helena.albergaria@lneg.pt.
FAU - Prista, Catarina
AU  - Prista C
AD  - Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior 
      de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal. 
      cprista@isa.ulisboa.pt.
LA  - eng
GR  - SFRH/ BD/ 89673/ 2012/Funda??o para a Ci?ncia e a Tecnologia (PT)/
GR  - FCOMP-01-0124-FEDER-014055/Funda??o para a Ci?ncia e a Tecnologia/
GR  - PTDC/BII-BIO/31761/2017/Funda??o para a Ci?ncia e a Tecnologia/
GR  - UID/Multi/04378/2013/Funda??o para a Ci?ncia e a Tecnologia/
GR  - UID/AGR/04129/2013/Funda??o para a Ci?ncia e a Tecnologia/
PT  - Journal Article
DEP - 20190208
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Biological Control Agents)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - *Biological Control Agents
MH  - *Brettanomyces
MH  - *Dekkera
MH  - Ethanol/metabolism
MH  - *Fermentation
MH  - *Food Microbiology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Industrial Microbiology
MH  - Microorganisms, Genetically-Modified
MH  - Saccharomyces cerevisiae/*enzymology/genetics
MH  - Saccharomyces cerevisiae Proteins/metabolism
MH  - Wine/microbiology
OTO - NOTNLM
OT  - Antimicrobial peptides, glyceraldehyde 3-phosphate dehydrogenase
OT  - Bioethanol
OT  - Fuel-ethanol
OT  - Genetically-modified yeasts
OT  - Microbial contamination
OT  - Preservatives
OT  - Wine
EDAT- 2019/02/09 06:00
MHDA- 2019/07/19 06:00
CRDT- 2019/02/09 06:00
PHST- 2018/11/16 00:00 [received]
PHST- 2019/01/21 00:00 [accepted]
PHST- 2019/01/18 00:00 [revised]
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2019/07/19 06:00 [medline]
PHST- 2019/02/09 06:00 [entrez]
AID - 10.1007/s00253-019-09657-7 [pii]
AID - 10.1007/s00253-019-09657-7 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2019 Apr;103(7):3073-3083. doi: 
      10.1007/s00253-019-09657-7. Epub 2019 Feb 8.