PMID- 30734231
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20200422
IS  - 1708-0428 (Electronic)
IS  - 0960-8923 (Linking)
VI  - 29
IP  - 6
DP  - 2019 Jun
TI  - Duodenojejunal Bypass Plus Sleeve Gastrectomy Reduces Infiltration of Macrophages 
      and Secretion of TNF-alpha in the Visceral White Adipose Tissue of Goto-Kakizaki 
      Rats.
PG  - 1742-1750
LID - 10.1007/s11695-019-03755-1 [doi]
AB  - BACKGROUND: Current studies indicate that inflammation of white adipose tissue 
      (WAT) is a pathogenic characteristic of insulin resistance. However, the 
      significance of visceral WAT inflammation after bariatric surgery remains 
      unclear. METHODS: Duodenojejunal bypass plus sleeve gastrectomy (DJB-SG) was 
      performed on Goto-Kakisaki rats. Weight, fasting blood glucose (FBG), and 
      homeostatic model assessment of insulin resistance (HOMA-IR) in the DJB-SG group 
      were compared to those in a sham surgery (SHAM) group every 2 weeks. The results 
      of an oral glucose tolerance test (OGTT) and the volume of visceral adipose 
      tissue (Visc.Fat) were compared before and 8 weeks postsurgery. Eight weeks after 
      surgery, the rats were sacrificed and visceral WAT collected from the greater 
      omentum. Tumor necrosis factor-alpha (TNF-alpha) and cluster of differentiation 68 (CD68) 
      expression in the WAT were evaluated in paraffin-embedded sections by 
      immunohistochemistry. RESULTS: Compared with the SHAM group, the DJB-SG group 
      demonstrated a significant reduction in weight, FBG, and HOMA-IR (P < 0.05), with 
      elevation of insulin levels (P < 0.05) from 4 weeks after surgery. OGTT and the 
      quantity of Visc.Fat were significantly reduced (P < 0.05) 8 weeks after surgery. 
      Moreover, the expression of TNF-alpha and CD68 in the visceral white adipose tissue 
      was significantly lower 8 weeks after surgery (P < 0.05). CONCLUSIONS: The DJB-SG 
      model established in Goto-Kakisaki rats achieved anticipated efficacy. Reduced 
      TNF-alpha-related inflammation in visceral WAT may result in improved insulin 
      resistance.
FAU - Yu, Hao
AU  - Yu H
AD  - Special Medical Service Center, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, 510280, China.
FAU - Song, Zhigao
AU  - Song Z
AD  - Southern Medical University, Guangzhou, 510515, China.
AD  - Department of Metabolic Surgery,, Guangzhou General Hospital of Guangzhou 
      Military Command, Guangzhou, 510515, China.
FAU - Zhang, Hongbin
AU  - Zhang H
AD  - Department of Metabolic Surgery,, Guangzhou General Hospital of Guangzhou 
      Military Command, Guangzhou, 510515, China.
FAU - Zheng, Kehong
AU  - Zheng K
AD  - Special Medical Service Center, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, 510280, China.
FAU - Zhan, Junfang
AU  - Zhan J
AD  - Physical Examination Center, Guangzhou First People's Hospital, Guangzhou, 
      510000, China.
FAU - Luo, Qing
AU  - Luo Q
AD  - Southern Medical University, Guangzhou, 510515, China.
FAU - Sun, Jingbo
AU  - Sun J
AD  - Department of Metabolic Surgery,, Guangzhou General Hospital of Guangzhou 
      Military Command, Guangzhou, 510515, China.
FAU - Liang, Li
AU  - Liang L
AD  - Southern Medical University, Guangzhou, 510515, China.
FAU - Dai, Xiaojiang
AU  - Dai X
AD  - Department of Metabolic Surgery, UDM Medical Group, Guangzhou, 510515, China. 
      drdaixj@163.com.
AD  - Abdominal surgery, Jinshazhou Hospital of Guangzhou University of Traditional 
      Chinese Medicine, Guangzhou, 510515, China. drdaixj@163.com.
FAU - Wu, Liangping
AU  - Wu L
AUID- ORCID: 0000-0001-9457-6921
AD  - Southern Medical University, Guangzhou, 510515, China. drwulp@163.com.
AD  - Department of Metabolic Surgery, UDM Medical Group, Guangzhou, 510515, China. 
      drwulp@163.com.
AD  - Abdominal surgery, Jinshazhou Hospital of Guangzhou University of Traditional 
      Chinese Medicine, Guangzhou, 510515, China. drwulp@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obes Surg
JT  - Obesity surgery
JID - 9106714
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Type 2/*metabolism/surgery
MH  - Disease Models, Animal
MH  - Duodenum/surgery
MH  - *Gastrectomy
MH  - *Gastric Bypass
MH  - Glucose Tolerance Test
MH  - Insulin Resistance
MH  - Intra-Abdominal Fat/*metabolism
MH  - Jejunum/surgery
MH  - Macrophages/*physiology
MH  - Male
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*metabolism
OTO - NOTNLM
OT  - DJB-SG
OT  - Inflammation
OT  - Insulin resistance
OT  - T2D
EDAT- 2019/02/09 06:00
MHDA- 2020/04/23 06:00
CRDT- 2019/02/09 06:00
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2019/02/09 06:00 [entrez]
AID - 10.1007/s11695-019-03755-1 [pii]
AID - 10.1007/s11695-019-03755-1 [doi]
PST - ppublish
SO  - Obes Surg. 2019 Jun;29(6):1742-1750. doi: 10.1007/s11695-019-03755-1.