PMID- 30734853
OWN - NLM
STAT- MEDLINE
DCOM- 20190501
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 10
DP  - 2019 Apr
TI  - Enantioseparation of chiral beta-blockers using polynorepinephrine-coated 
      nanoparticles and chiral capillary electrophoresis.
PG  - 2121-2129
LID - 10.1007/s00216-019-01641-4 [doi]
AB  - A method of combining magnetic solid-phase separation (MSPE) and chiral capillary 
      electrophoresis (CE) is developed for enantioseparation of trace amounts of 
      beta-blockers. Polynorepinephrine-functionalized magnetic nanoparticles 
      (polyNE-MNPs) are synthesized and applied to simultaneously extract three 
      beta-blockers (carteolol, metoprolol, and betaxolol). The prepared polyNE-MNPs are 
      spherical with a diameter of 198 +/- 17 nm and the thickness of the polyNE coating 
      is about 14 nm. PolyNE possesses abundant catechol hydroxyl and secondary amine 
      groups, endowing the MNPs with excellent hydrophilicity. Under the optimum 
      conditions, the extraction efficiencies of polyNE-MNPs for beta-blockers are in the 
      range of 89.6 to 100%, with relative standard deviations (RSDs) below 3.5%. The 
      extraction process can be finished in 4 min. Field-enhanced sample injection 
      (FESI) in chiral CE is constructed to further enhance the sensitivities of 
      beta-blocker enantiomers. The limits of detection for beta-blocker enantiomers by the 
      FESI-CE with polyNE-MNPs are in the range of 0.401 to 1.59 ng mL(-1). The 
      practicability of this method in real samples is evaluated by analysis of human 
      urine samples. The recoveries for each enantiomer of beta-blockers in the real 
      samples range from 89.5 to 92.8%, with RSDs ranging from 0.37 to 5.9%. The whole 
      detection process can be finished in less than 0.5 h. The method demonstrates its 
      great potential in the pharmacokinetic and pharmacodynamic studies of chiral 
      drugs in humans. Graphical abstract ᅟ.
FAU - Wu, Jia
AU  - Wu J
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, Guangdong, China.
FAU - Xiao, Xue
AU  - Xiao X
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, Guangdong, China.
FAU - Li, Zhenqun
AU  - Li Z
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, Guangdong, China.
FAU - Jia, Li
AU  - Jia L
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, Guangdong, China. jiali@scnu.edu.cn.
LA  - eng
GR  - 201805010002/the Scientific and Technological Planning Project of Guangzhou City/
GR  - 21675056/National Natural Science Foundation of China/
PT  - Journal Article
PT  - Validation Study
DEP - 20190208
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (Magnetite Nanoparticles)
RN  - 8NF31401XG (Carteolol)
RN  - GEB06NHM23 (Metoprolol)
RN  - O0ZR1R6RZ2 (Betaxolol)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Adrenergic beta-Antagonists/*isolation & purification/*urine
MH  - Betaxolol/isolation & purification/urine
MH  - Carteolol/isolation & purification/urine
MH  - Electrophoresis, Capillary/instrumentation/*methods
MH  - Equipment Design
MH  - Humans
MH  - Limit of Detection
MH  - Magnetics/instrumentation/methods
MH  - Magnetite Nanoparticles/*chemistry/ultrastructure
MH  - Metoprolol/isolation & purification/urine
MH  - Norepinephrine/*analogs & derivatives
MH  - Solid Phase Microextraction/instrumentation/methods
MH  - Stereoisomerism
OTO - NOTNLM
OT  - Capillary electrophoresis
OT  - Enantioseparation
OT  - Field-enhanced sample injection
OT  - Magnetic separation
OT  - Polynorepinephrine
OT  - beta-Blocker
EDAT- 2019/02/09 06:00
MHDA- 2019/05/02 06:00
CRDT- 2019/02/09 06:00
PHST- 2018/11/01 00:00 [received]
PHST- 2019/01/24 00:00 [accepted]
PHST- 2019/01/11 00:00 [revised]
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2019/05/02 06:00 [medline]
PHST- 2019/02/09 06:00 [entrez]
AID - 10.1007/s00216-019-01641-4 [pii]
AID - 10.1007/s00216-019-01641-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Apr;411(10):2121-2129. doi: 10.1007/s00216-019-01641-4. 
      Epub 2019 Feb 8.