PMID- 30735047
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 35
IP  - 9
DP  - 2019 Mar 5
TI  - Tunable Phospholipid Nanopatterns Mediated by Cholesterol with Sub-3 nm Domain 
      Size.
PG  - 3383-3390
LID - 10.1021/acs.langmuir.8b03075 [doi]
AB  - The interactions between phospholipids and cholesterol have been extensively 
      studied in the aqueous systems because of their vital functionalities in the cell 
      membrane. In this study, instead of the self-assembly in water, we explored the 
      microphase-separated structures of phospholipids in bulk and thin films in the 
      absence of solvents and created a series of ordered nanostructures by 
      incorporation of cholesterol into phospholipids. Three zwitterionic two-tailed 
      phospholipids, that is, phosphatidylcholines (PCs), with different numbers of 
      double bonds on the hydrocarbon tails were investigated, including egg PC, 
      1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC), and 1,2-dipalmitoyl- 
      sn-glycero-3-phosphocholine (DPPC). We find that the nanostructures are highly 
      dependent on the conformation of the tails on the PCs, which can be tailored by 
      the number of double bonds on tails and the molar ratio of cholesterol to PC. By 
      changing the molar ratio, egg PC with one double bond organizes into rich 
      microdomains, including lamellae, spheres, and cylinders, whereas DOPC with two 
      double bonds form spheres and cylinders and DPPC with no double bond forms 
      lamellae only. The sizes of the microdomains are less than 3 nm, smaller than 
      those of typical block copolymers. The biomolecule-based nanopatterns developed 
      in this work provide a platform toward future applications of nanotechnology and 
      biotechnology.
FAU - Lee, Chia-Chun
AU  - Lee CC
AD  - Institute of Polymer Science and Engineering and Advanced Research Center for 
      Green Materials Science and Technology , National Taiwan University , Taipei 
      10617 , Taiwan.
FAU - Lin, Chen-Shin
AU  - Lin CS
AD  - Institute of Polymer Science and Engineering and Advanced Research Center for 
      Green Materials Science and Technology , National Taiwan University , Taipei 
      10617 , Taiwan.
FAU - Tung, Shih-Huang
AU  - Tung SH
AUID- ORCID: 0000-0002-6787-4955
AD  - Institute of Polymer Science and Engineering and Advanced Research Center for 
      Green Materials Science and Technology , National Taiwan University , Taipei 
      10617 , Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190220
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
SB  - IM
EDAT- 2019/02/09 06:00
MHDA- 2019/02/09 06:01
CRDT- 2019/02/09 06:00
PHST- 2019/02/09 06:00 [pubmed]
PHST- 2019/02/09 06:01 [medline]
PHST- 2019/02/09 06:00 [entrez]
AID - 10.1021/acs.langmuir.8b03075 [doi]
PST - ppublish
SO  - Langmuir. 2019 Mar 5;35(9):3383-3390. doi: 10.1021/acs.langmuir.8b03075. Epub 
      2019 Feb 20.