PMID- 30738249
OWN - NLM
STAT- MEDLINE
DCOM- 20190501
LR  - 20190501
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 107
DP  - 2019 Mar
TI  - CD226 is involved in megakaryocyte activation and early-stage differentiation.
PG  - 123-131
LID - S0161-5890(18)30801-0 [pii]
LID - 10.1016/j.molimm.2019.01.013 [doi]
AB  - This study was conducted to investigate the effect of CD226 on the 
      differentiation, activation, and polyploidization of megakaryocytes (MKs) and 
      explore the potential mechanism. Dami (megakaryocyte line) cell maturation was 
      induced by phorbol 12-myristate 13-acetate. CD226 was silenced by infection with 
      a CD226-specific shRNA lentiviral vector. The mRNA level of CD226 was detected by 
      qRT-PCR. The expressions of Dami cells surface CD226, MK specific markers CD41 
      and CD62P, and DNA ploidy in Dami cells and CD226 knockdown (KD) cells were 
      evaluated by flow cytometry. The effect of CD226 on the expression of 
      megakaryocyte-associated transcription factors was measured by western blot and 
      confocal analysis. Transfection with CD226 shRNA lentivirus dramatically 
      decreased the level of CD226 and expression of CD62 P in Dami cells. Silencing of 
      CD226 caused morphological changes and differentiation retardation in low-ploidy 
      MK. Furthermore, CD226 knockout (KO) mice exhibited increased 2N-4N low-ploidy MK 
      and decreased >/=8N polyploidy. Interestingly, silencing of CD226 in megakaryocytic 
      cells down-regulated the expression of early stage transcription factors includes 
      GATA-binding factor 1 (GATA-1) and friend leukemia integration 1 (FLI-1), but not 
      late-stage nuclear factor, erythroid 2 (NF-E2). CD226 is involved in MKs 
      activation and polyploidy cell cycle control.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Zhang, Jinxue
AU  - Zhang J
AD  - Orthopedic Department of Tangdu Hospital, the Fourth Military Medical University, 
      #1 Xinsi Road, Xi'an, PR China.
FAU - Zhuang, Ran
AU  - Zhuang R
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military 
      Medical University, #1 Changle Xi Road, Xi'an, PR China.
FAU - Zhang, Xuexin
AU  - Zhang X
AD  - Transplant Immunology Laboratory, the Fourth Military Medical University, #1 
      Changle Xi Road, Xi'an, PR China.
FAU - Hu, Wei
AU  - Hu W
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China.
FAU - Cheng, Kun
AU  - Cheng K
AD  - Transplant Immunology Laboratory, the Fourth Military Medical University, #1 
      Changle Xi Road, Xi'an, PR China.
FAU - Jiang, Dongxu
AU  - Jiang D
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military 
      Medical University, #1 Changle Xi Road, Xi'an, PR China.
FAU - Shen, Shen
AU  - Shen S
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military 
      Medical University, #1 Changle Xi Road, Xi'an, PR China.
FAU - Zhang, Yun
AU  - Zhang Y
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China.
FAU - Ding, Yong
AU  - Ding Y
AD  - Orthopedic Department of Tangdu Hospital, the Fourth Military Medical University, 
      #1 Xinsi Road, Xi'an, PR China. Electronic address: dinyonza@fmmu.edu.cn.
FAU - Zhang, Yuan
AU  - Zhang Y
AD  - Department of Immunology, the fourth Military Medical University, #1 Changle Xi 
      Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military 
      Medical University, #1 Changle Xi Road, Xi'an, PR China. Electronic address: 
      zhangyuan@fmmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190206
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD226 antigen)
RN  - 0 (FLI1 protein, human)
RN  - 0 (Fli1 protein, mouse)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (GATA1 protein, human)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (NF-E2 Transcription Factor, p45 Subunit)
RN  - 0 (NFE2 protein, human)
RN  - 0 (Nfe2 protein, mouse)
RN  - 0 (P-Selectin)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (SELP protein, human)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, T-Lymphocyte/genetics/*immunology
MH  - Cell Differentiation/drug effects/genetics/*immunology
MH  - Cell Line
MH  - GATA1 Transcription Factor/genetics/immunology
MH  - Gene Expression Regulation/drug effects/*immunology
MH  - Humans
MH  - Male
MH  - Megakaryocytes/cytology/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - NF-E2 Transcription Factor, p45 Subunit/genetics/immunology
MH  - P-Selectin/genetics/immunology
MH  - Ploidies
MH  - Proto-Oncogene Protein c-fli-1/genetics/immunology
MH  - Tetradecanoylphorbol Acetate/pharmacology
OTO - NOTNLM
OT  - CD226
OT  - Differentiation
OT  - FLI-1
OT  - GATA-1
OT  - Megakaryocyte
EDAT- 2019/02/10 06:00
MHDA- 2019/05/02 06:00
CRDT- 2019/02/10 06:00
PHST- 2018/09/22 00:00 [received]
PHST- 2019/01/21 00:00 [accepted]
PHST- 2019/02/10 06:00 [pubmed]
PHST- 2019/05/02 06:00 [medline]
PHST- 2019/02/10 06:00 [entrez]
AID - S0161-5890(18)30801-0 [pii]
AID - 10.1016/j.molimm.2019.01.013 [doi]
PST - ppublish
SO  - Mol Immunol. 2019 Mar;107:123-131. doi: 10.1016/j.molimm.2019.01.013. Epub 2019 
      Feb 6.