PMID- 30738855
OWN - NLM
STAT- MEDLINE
DCOM- 20191224
LR  - 20191224
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 404
DP  - 2019 Apr 15
TI  - Reorganization of Septins Modulates Synaptic Transmission at Neuromuscular 
      Junctions.
PG  - 91-101
LID - S0306-4522(19)30087-9 [pii]
LID - 10.1016/j.neuroscience.2019.01.060 [doi]
AB  - Septins (Sept) are highly conserved Guanosine-5'-triphosphate (GTP)-binding 
      cytoskeletal proteins involved in neuronal signaling in the central nervous 
      system but their involvement in signal transmission in peripheral synapses 
      remains unclear. Sept5 and Sept9 proteins were detected in mouse peripheral 
      neuromuscular junctions by immunofluorescence with a greater degree of 
      co-localization with presynaptic than postsynaptic membranes. Preincubation of 
      neuromuscular junction preparations with the inhibitor of Sept dynamics, 
      forchlorfenuron (FCF), decreased co-localization of Sept with presynaptic 
      membranes. FCF introduced ex vivo or in vivo had no effect on the amplitude of 
      the spontaneous endplate currents (EPCs), indicating the absence of postsynaptic 
      effects of FCF. However, FCF decreased acetylcholine (ACh) quantal release in 
      response to nerve stimulation, reduced the amplitude of evoked quantal currents 
      and decreased the number of quanta with long synaptic delays, demonstrating the 
      presynaptic action of FCF. Nevertheless, FCF had no effect on the amplitude of 
      calcium transient in nerve terminals, as detected by calcium-sensitive dye, and 
      slightly decreased the ratio of the second response amplitude to the first one in 
      paired-pulse experiments. These results suggest that FCF-induced decrease in ACh 
      quantal secretion is not due to a decrease in Ca(2+) influx but is likely related 
      to the impairment of later stages occurring after Ca(2+) entry, such as 
      trafficking, docking or membrane fusion of synaptic vesicles. Therefore, Sept9 
      and Sept5 are abundantly expressed in presynaptic membranes, and disruption of 
      Sept dynamics suppresses the evoked synchronous and delayed asynchronous quantal 
      release of ACh, strongly suggesting an important role of Sept in the regulation 
      of neurotransmission in peripheral synapses.
CI  - Copyright (c) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Nurullin, Leniz F
AU  - Nurullin LF
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia; Kazan State 
      Medical University, Kazan, Russia; Open Laboratory of Neuropharmacology, Kazan 
      Federal University, Kazan, Russia. Electronic address: rus_science@yahoo.com.
FAU - Khuzakhmetova, Venera F
AU  - Khuzakhmetova VF
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia; Open 
      Laboratory of Neuropharmacology, Kazan Federal University, Kazan, Russia.
FAU - Khaziev, Eduard F
AU  - Khaziev EF
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia; Open 
      Laboratory of Neuropharmacology, Kazan Federal University, Kazan, Russia; A.N. 
      Tupolev Kazan National Research Technical University, Kazan, Russia.
FAU - Samigullin, Dmitry V
AU  - Samigullin DV
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia; Open 
      Laboratory of Neuropharmacology, Kazan Federal University, Kazan, Russia; A.N. 
      Tupolev Kazan National Research Technical University, Kazan, Russia.
FAU - Tsentsevitsky, Andrei N
AU  - Tsentsevitsky AN
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia.
FAU - Skorinkin, Andrei I
AU  - Skorinkin AI
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia.
FAU - Bukharaeva, Ellya A
AU  - Bukharaeva EA
AD  - Laboratory of Biophysics Of Synaptic Processes, Kazan Institute of Biochemistry 
      and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia; Open 
      Laboratory of Neuropharmacology, Kazan Federal University, Kazan, Russia.
FAU - Vagin, Olga
AU  - Vagin O
AD  - Department of Physiology, David Geffen School of Medicine at UCLA, Los Angeles, 
      CA, USA; Veterans Administration Greater Los Angeles Healthcare System, Los 
      Angeles, CA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190208
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - EC 3.6.1.- (Septins)
SB  - IM
MH  - Animals
MH  - Diaphragm/innervation/physiology
MH  - Evoked Potentials, Motor/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neuromuscular Junction/*pathology
MH  - Phrenic Nerve/physiology
MH  - Septins/*physiology
MH  - Synapses/*physiology
MH  - Synaptic Transmission/*physiology
OTO - NOTNLM
OT  - acetylcholine quantal release
OT  - calcium imaging
OT  - neuromuscular junction
OT  - septins
EDAT- 2019/02/11 06:00
MHDA- 2019/12/25 06:00
CRDT- 2019/02/11 06:00
PHST- 2018/07/06 00:00 [received]
PHST- 2019/01/27 00:00 [revised]
PHST- 2019/01/29 00:00 [accepted]
PHST- 2019/02/11 06:00 [pubmed]
PHST- 2019/12/25 06:00 [medline]
PHST- 2019/02/11 06:00 [entrez]
AID - S0306-4522(19)30087-9 [pii]
AID - 10.1016/j.neuroscience.2019.01.060 [doi]
PST - ppublish
SO  - Neuroscience. 2019 Apr 15;404:91-101. doi: 10.1016/j.neuroscience.2019.01.060. 
      Epub 2019 Feb 8.