PMID- 30738873
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1098-8823 (Print)
IS  - 1098-8823 (Linking)
VI  - 141
DP  - 2019 Apr
TI  - Leukotriene B(4) modulation of murine dendritic cells affects adaptive immunity.
PG  - 34-39
LID - S1098-8823(18)30117-5 [pii]
LID - 10.1016/j.prostaglandins.2019.02.001 [doi]
AB  - Dendritic cells (DCs) link innate and adaptive immunity. The microenvironment 
      generated during the innate immunity affects DCs and the type of adaptive 
      immunity generated. Lipid mediators are released early in inflammation and could 
      modify the functional state of DCs. Leukotriene B(4) (LTB(4)) has a wide range of 
      effects on macrophages and in the present study we investigated if it also 
      affects DCs. Murine bone marrow-derived DCs were employed and it was found that 
      stimulation of DCs with LTB4 (10 nM) increased the gene expression of the high 
      affinity receptor BLT-1 but not of BLT-2. It also increased the co-stimulatory 
      molecule CD86 expression but did not affect CD80 and CD40. LTB(4)-stimulated DCs 
      acquired the capacity to present antigen to T lymphocytes, evidenced by 
      antigen-specific proliferation of CD4(+) lymphocytes in co-cultures of 
      ovalbumin-loaded DCs with DO11.10 splenocytes. LTB(4)-stimulated DCs induced Treg 
      proliferation and increased Th2 cytokine IL-13 in the co-cultures. Expression of 
      transcription factor genes, Gata3 and Foxp3 (Th2 and Treg, respectively) were 
      also found increased. However, the expression of Th1 transcription factor (Tbet) 
      and Th17 (RorgammaT) were not affected. These results indicate that LTB(4) affects 
      DCs and modulates the type of adaptive immune response.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Pires-Lapa, Marco Antonio
AU  - Pires-Lapa MA
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. Electronic address: marcolapabio@gmail.com.
FAU - Koga, Marianna Mainardi
AU  - Koga MM
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. Electronic address: marianna.koga@unil.ch.
FAU - da Silva, Ildefonso Alves Jr
AU  - da Silva IA Jr
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. Electronic address: iasjr@usp.br.
FAU - Filgueiras, Luciano Ribeiro
AU  - Filgueiras LR
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. Electronic address: luciano.ribeiro@usp.br.
FAU - Jancar, Sonia
AU  - Jancar S
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. Electronic address: sojancar@icb.usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190208
PL  - United States
TA  - Prostaglandins Other Lipid Mediat
JT  - Prostaglandins & other lipid mediators
JID - 9808648
RN  - 0 (Immunologic Factors)
RN  - 0 (Lipopolysaccharides)
RN  - 1HGW4DR56D (Leukotriene B4)
SB  - IM
MH  - Adaptive Immunity/*drug effects
MH  - Animals
MH  - Antigen Presentation/drug effects
MH  - Dendritic Cells/*drug effects/*immunology
MH  - Immunologic Factors/*pharmacology
MH  - Leukotriene B4/*pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
OTO - NOTNLM
OT  - Adaptive immunity
OT  - Dendritic cells
OT  - Leukotriene B4
EDAT- 2019/02/11 06:00
MHDA- 2019/04/09 06:00
CRDT- 2019/02/11 06:00
PHST- 2018/08/21 00:00 [received]
PHST- 2019/01/18 00:00 [revised]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2019/02/11 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
PHST- 2019/02/11 06:00 [entrez]
AID - S1098-8823(18)30117-5 [pii]
AID - 10.1016/j.prostaglandins.2019.02.001 [doi]
PST - ppublish
SO  - Prostaglandins Other Lipid Mediat. 2019 Apr;141:34-39. doi: 
      10.1016/j.prostaglandins.2019.02.001. Epub 2019 Feb 8.