PMID- 30740929
OWN - NLM
STAT- MEDLINE
DCOM- 20200515
LR  - 20200515
IS  - 2059-2310 (Electronic)
IS  - 2059-2302 (Linking)
VI  - 93
IP  - 2-3
DP  - 2019 Feb
TI  - High-resolution characterization of 12 classical and non-classical HLA loci in 
      Southern Brazilians.
PG  - 80-88
LID - 10.1111/tan.13488 [doi]
AB  - The human leukocyte antigen (HLA) are the most polymorphic genes in the human 
      genome. Because of their importance for antigen recognition, HLA molecules play a 
      central role in host defense and graft rejection upon transplantation. The aim of 
      this study was to characterize allelic diversity of the classical HLA genes 
      HLA-A, -B, -C, -DRA, -DRB1, -DQA1, -DQB1, -DPA1, -DPB1, and the non-classical 
      class I genes HLA-E, -F and -G at high-resolution for a population of 
      predominantly European ancestry from Curitiba, Brazil. Genotyping of 108 
      individuals was performed by next-generation sequencing on the MiSeq platform and 
      also by Sanger sequencing. The genotype distributions of all loci were in 
      accordance with Hardy-Weinberg equilibrium (P > 0.05) and a total of 202 HLA 
      variants at second field resolution were observed for the 12 loci. The strongest 
      linkage disequilibrium (r(2) = 1.0, P < 10(-5) ) was observed for the following 
      pairs of alleles: HLA-B*42:01:01 ~ HLA-DRB1*03:02:01; HLA-B*14:02:01 ~ 
      HLA-C*08:02:01; B*42:01:01 ~ HLA-C*17:01:01; HLA-DRB1*03:01:01 ~ 
      HLA-DQB1*02:01:01 ~ DRB1*03:01:01 ~ HLA-DQB1*02:01:01; DRB1*13:01:01~ 
      HLA-DQB1*06:03:01 and HLA-DRB1*09:01:02 ~ HLA-DQA1*03:02. This is the first study 
      to characterize all 12 HLA genes at high resolution in a single population. On 
      the basis of the allelic frequencies of worldwide populations and principal 
      component analysis, we confirmed the similarity of the study population to 
      European and other Euro-descendant populations.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Castro, Mariana de Sousa
AU  - Castro MS
AD  - Laboratorio de Genetica Molecular Humana, Departamento de Genetica, Universidade 
      Federal do Parana, Curitiba, Brazil.
FAU - Issler, Hellen C
AU  - Issler HC
AD  - Laboratorio de Genetica Molecular Humana, Departamento de Genetica, Universidade 
      Federal do Parana, Curitiba, Brazil.
FAU - Gelmini, Georgia F
AU  - Gelmini GF
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Departamento de Genetica, 
      Universidade Federal do Parana, Curitiba, Brazil.
FAU - de Miranda, Bruna L M
AU  - de Miranda BLM
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Departamento de Genetica, 
      Universidade Federal do Parana, Curitiba, Brazil.
FAU - Calonga-Solis, Veronica
AU  - Calonga-Solis V
AD  - Laboratorio de Genetica Molecular Humana, Departamento de Genetica, Universidade 
      Federal do Parana, Curitiba, Brazil.
FAU - Schmidt, Alexander H
AU  - Schmidt AH
AD  - DKMS Life Science Lab, Dresden, Germany.
AD  - DKMS, Tubingen, Germany.
FAU - Stein, Anke
AU  - Stein A
AD  - DKMS Life Science Lab, Dresden, Germany.
FAU - Bicalho, Maria da Graca
AU  - Bicalho MDG
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Departamento de Genetica, 
      Universidade Federal do Parana, Curitiba, Brazil.
FAU - Petzl-Erler, Maria Luiza
AU  - Petzl-Erler ML
AUID- ORCID: 0000-0002-0345-5276
AD  - Laboratorio de Genetica Molecular Humana, Departamento de Genetica, Universidade 
      Federal do Parana, Curitiba, Brazil.
FAU - Augusto, Danillo G
AU  - Augusto DG
AUID- ORCID: 0000-0001-5665-8547
AD  - Laboratorio de Genetica Molecular Humana, Departamento de Genetica, Universidade 
      Federal do Parana, Curitiba, Brazil.
LA  - eng
GR  - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/International
GR  - Fundacao Araucaria/International
GR  - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - HLA
JT  - HLA
JID - 101675570
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Alleles
MH  - Brazil
MH  - Gene Frequency/genetics
MH  - *Genetic Loci
MH  - Geography
MH  - Haplotypes/genetics
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Linkage Disequilibrium/genetics
MH  - Principal Component Analysis
OTO - NOTNLM
OT  - Curitiba
OT  - NGS
OT  - human leukocyte antigen
OT  - population genetics
EDAT- 2019/02/12 06:00
MHDA- 2020/05/16 06:00
CRDT- 2019/02/12 06:00
PHST- 2018/08/25 00:00 [received]
PHST- 2019/02/05 00:00 [revised]
PHST- 2019/02/08 00:00 [accepted]
PHST- 2019/02/12 06:00 [pubmed]
PHST- 2020/05/16 06:00 [medline]
PHST- 2019/02/12 06:00 [entrez]
AID - 10.1111/tan.13488 [doi]
PST - ppublish
SO  - HLA. 2019 Feb;93(2-3):80-88. doi: 10.1111/tan.13488.