PMID- 30741293 OWN - NLM STAT- MEDLINE DCOM- 20190614 LR - 20190614 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 10 IP - 2 DP - 2019 Feb 20 TI - Suppressive effects of the supercritical-carbon dioxide fluid extract of Chrysanthemum indicum on chronic unpredictable mild stress-induced depressive-like behavior in mice. PG - 1212-1224 LID - 10.1039/c8fo02474j [doi] AB - The aim of the present study was to explore whether the supercritical-carbon dioxide fluid extract from flowers and buds of Chrysanthemum indicum (SEC) exhibits antidepressant-like effects in a chronic unpredictable mild stress (CUMS)-induced mice model. Firstly, SEC was found to reverse a CUMS-induced decrease in the body weight gain in mice. Next, SEC was found to alleviate CUMS-induced depressive-like behavior, evidenced by the reversal of the decrease in the sucrose consumption in the sucrose preference test (SPT), the increase in the locomotor activity in the open field test (OPF), and the alleviation of immobility duration in both the forced swimming test (FST), and tail-suspension test (TST). SEC also attenuated CUMS-induced hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis by decreasing the levels of serum corticosterone and (CORT) and adrenocorticotropic hormone (ACTH), and hypothalamus corticotrophin-releasing hormone (CRH). In addition, SEC was found to suppress the expression of pro-inflammatory cytokines, including the tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6 in the hippocampal of CUMS mice. Interestingly, further investigations demonstrated that SEC inhibited CUMS-induced activation of the nuclear factor kappa B (NF-kappaB) and NOD-like receptor protein 3 (NLRP3) inflammasomes pathways but upregulated brain-derived neurotrophic factor (BDNF) expression and promoted phosphorylation of extracellular signal-regulated kinase (ERK) and cAMP-response element-binding protein (CREB) in hippocampal. In summary, SEC was able to alleviate depressive-like behavior in a CUMS-induced mice model, accompanied by inhibitory roles in the hyperactivity of the HPA axis and pro-inflammatory cytokine expression. Modulating the NF-kappaB/NLRP3 and BDNF/CREB/ERK pathways contributed to SEC-mediated antidepressant-like effects. FAU - Wu, Xiaoli AU - Wu X AD - The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. wuxiaolily@126.com. FAU - Tang, Benqin AU - Tang B FAU - Liao, Xian AU - Liao X FAU - Su, Ziren AU - Su Z FAU - Lee, Simon Ming-Yuen AU - Lee SM FAU - Cai, Yefeng AU - Cai Y FAU - Li, Chuwen AU - Li C LA - eng PT - Journal Article PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Antidepressive Agents) RN - 0 (Bdnf protein, mouse) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Plant Extracts) RN - 142M471B3J (Carbon Dioxide) RN - 57-50-1 (Sucrose) SB - IM MH - Animals MH - Antidepressive Agents/chemistry/*pharmacology MH - Body Weight/drug effects MH - Brain-Derived Neurotrophic Factor/genetics/metabolism MH - Carbon Dioxide MH - Chromatography, Supercritical Fluid MH - Chrysanthemum/*chemistry MH - Depression/*drug therapy/etiology MH - Feeding Behavior/drug effects MH - Gene Expression Regulation/drug effects MH - Male MH - Mice MH - Mice, Inbred BALB C MH - Motor Activity MH - Phytotherapy MH - Plant Extracts/chemistry/*pharmacology MH - *Stress, Physiological MH - Sucrose EDAT- 2019/02/12 06:00 MHDA- 2019/06/15 06:00 CRDT- 2019/02/12 06:00 PHST- 2019/02/12 06:00 [pubmed] PHST- 2019/06/15 06:00 [medline] PHST- 2019/02/12 06:00 [entrez] AID - 10.1039/c8fo02474j [doi] PST - ppublish SO - Food Funct. 2019 Feb 20;10(2):1212-1224. doi: 10.1039/c8fo02474j.