PMID- 30741488
OWN - NLM
STAT- MEDLINE
DCOM- 20190829
LR  - 20190829
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 47
IP  - 6
DP  - 2019 Jun
TI  - Genotyping and cytomorphological subtyping of lung adenocarcinoma based on 
      liquid-based cytology.
PG  - 564-570
LID - 10.1002/dc.24154 [doi]
AB  - BACKGROUND: Liquid-based cytology (LBC) samples allow immunohistochemistry (IHC), 
      fluorescence in situ hybridization (FISH), and molecular testing of nucleic acids 
      to be performed in the remaining fixed cells. The current study aimed to examine 
      the relationship between gene mutational status and cytomorphological features in 
      primary lung adenocarcinoma (ADC) using LBC materials. METHODS: Forty consecutive 
      patients with primary lung ADC underwent surgical resection in our hospital. 
      Cytological material was obtained by scraping the cut-surface of the lesion, and 
      samples were fixed and stored as LBC materials using CytoRich Red. Epidermal 
      growth factor receptor (EGFR) and Kirsten rat sarcoma viral oncogene homolog 
      (KRAS) mutations, anaplastic lymphoma kinase (ALK), and c-ros oncogene 1 (ROS1) 
      gene rearrangements were detected, and cytomorphological studies were performed. 
      RESULTS: Twenty cases (50%) were positive for EGFR mutation and four (10%) were 
      positive for KRAS mutation. ALK gene rearrangement was identified in one case 
      (2.5%) by IHC and FISH, and ROS1 gene rearrangement was identified in one case 
      (2.5%) by IHC and real-time polymerase chain reaction. The KRAS-positive group 
      included higher proportions of cases with an inflammatory background (100%), 
      predominantly papillary architecture (75%), and papillary-type ADC pattern (75%) 
      compared with the EGFR-positive group and the other group, which included ALK and 
      ROS1 gene rearrangements. CONCLUSIONS: LBC material is suitable for use in 
      molecular testing. Differences in major gene aberrations detected by this method 
      might predict specific cytomorphological features.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Tanaka, Ryota
AU  - Tanaka R
AUID- ORCID: 0000-0002-0615-0072
AD  - Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Sakamoto, Norihiko
AU  - Sakamoto N
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Suzuki, Hitomi
AU  - Suzuki H
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Tachibana, Keisei
AU  - Tachibana K
AD  - Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Ohtsuka, Kouki
AU  - Ohtsuka K
AD  - Department of Clinical Laboratory, Kyorin University School of Medicine, Tokyo, 
      Japan.
FAU - Kishimoto, Koji
AU  - Kishimoto K
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Fujiwara, Masachika
AU  - Fujiwara M
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Kamma, Hiroshi
AU  - Kamma H
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Shibahara, Junji
AU  - Shibahara J
AD  - Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Kondo, Haruhiko
AU  - Kondo H
AD  - Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
LA  - eng
GR  - JP16K10690/JSPS KAKENHI/
PT  - Journal Article
DEP - 20190211
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (ROS1 protein, human)
SB  - IM
CIN - Diagn Cytopathol. 2019 Oct;47(10):1119. PMID: 31407524
MH  - Adenocarcinoma of Lung/*diagnosis/*genetics/pathology
MH  - Aged
MH  - Anaplastic Lymphoma Kinase/genetics
MH  - Female
MH  - Gene Rearrangement
MH  - Genotyping Techniques/*methods
MH  - Humans
MH  - Liquid Biopsy
MH  - Lung/*pathology
MH  - Male
MH  - Mutation/genetics
MH  - Protein-Tyrosine Kinases/genetics
MH  - Proto-Oncogene Proteins/genetics
OTO - NOTNLM
OT  - Kirsten rat sarcoma viral oncogene homolog (KRAS)
OT  - cytomorphological subtypes
OT  - epidermal growth factor receptor (EGFR)
OT  - liquid-based cytology
OT  - lung adenocarcinoma
EDAT- 2019/02/12 06:00
MHDA- 2019/08/30 06:00
CRDT- 2019/02/12 06:00
PHST- 2018/09/21 00:00 [received]
PHST- 2019/01/04 00:00 [revised]
PHST- 2019/01/22 00:00 [accepted]
PHST- 2019/02/12 06:00 [pubmed]
PHST- 2019/08/30 06:00 [medline]
PHST- 2019/02/12 06:00 [entrez]
AID - 10.1002/dc.24154 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2019 Jun;47(6):564-570. doi: 10.1002/dc.24154. Epub 2019 Feb 
      11.